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Recombinant Mouse CCL19/MIP-3 beta Protein

 (39 Publications)
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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

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Catalog# & Formulation Size Price
Carrier Free
440-M3-025/CF
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Recombinant Mouse CCL19/MIP-3 beta Protein Summary

Details of Functionality
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.03‑0.1 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR7. The ED50 for this effect is 3-15 ng/mL.
Source
E. coli-derived mouse CCL19/MIP-3 beta protein
Gly26-Val107-Leu-Glu, with a substitution of Ser108LeuGlu
Accession #
N-terminal Sequence
Gly26
Protein/Peptide Type
Recombinant Proteins
Gene
Ccl19
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
9.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
440-M3 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL19/MIP-3 beta Protein

  • beta chemokine exodus-3
  • Beta-chemokine exodus-3
  • CC chemokine ligand 19
  • C-C motif chemokine 19
  • CCL19
  • chemokine (C-C motif) ligand 19
  • CKb11
  • EBI1-ligand chemokine
  • ELC
  • ELCMIP-3-beta
  • Epstein-Barr virus-induced molecule 1 ligand chemokine
  • exodus-3
  • Macrophage inflammatory protein 3 beta
  • macrophage inflammatory protein 3-beta
  • MGC34433
  • MIP3 beta
  • MIP-3 beta
  • MIP-3b
  • MIP3BCK beta-11
  • SCYA19EBI1 ligand chemokine
  • small inducible cytokine subfamily A (Cys-Cys), member 19
  • Small-inducible cytokine A19

Background

CCL19/MIP-3 beta , also known as ELC (EBI1-Ligand Chemokine), is a reported beta  chemokine that binds specifically to the chemokine receptor CCR‑7/EBI‑1/BLR‑2. Mouse (human) MIP-3 beta cDNA encodes a 108 (98) amino acid residue precursor protein with a predicted 25 (21) aa residue signal peptide that is cleaved to form the 83 (77) aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20 - 30% aa sequence identity). Mouse MIP-3 beta shares 83% aa sequence homology with human MIP-3 beta . MIP-3 beta  has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T-cell zone. The expression of MIP-3 beta is down‑regulated by the anti‑inflammatory cytokine IL‑10. Recombinant MIP-3 beta has been shown to be chemotactic for T cells and B cells. The MIP-3 beta receptor (CCR-7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR-7 is also strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7.

  1. Kim, C.H. et al. (1998) J. Immunol. 160:2418.
  2. Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
  3. Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
  4. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.

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Publications for CCL19/MIP-3 beta (440-M3)(39)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 2 applications: Bioassay, In Vivo.


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Bioassay
(36)
In Vivo
(3)
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Human
(1)
Mouse
(38)
Transgenic Mouse
(1)
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Showing Publications 1 - 10 of 39. Show All 39 Publications.
Publications using 440-M3 Applications Species
Xia, L;Komissarova, A;Jacover, A;Shovman, Y;Arcila-Barrera, S;Tornovsky-Babeay, S;Jaya Prakashan, MM;Nasereddin, A;Plaschkes, I;Nevo, Y;Shiff, I;Yosefov-Levi, O;Izhiman, T;Medvedev, E;Eilon, E;Wilensky, A;Yona, S;Parnas, O; Systematic identification of gene combinations to target in innate immune cells to enhance T cell activation Nature communications 2023-10-09 [PMID: 37813864] (Bioassay, Mouse, Transgenic Mouse) Bioassay Mouse, Transgenic Mouse
R Storjohann, B Gericke, J Reifenrath, T Herrmann, P Behrens, H Oltmanns, J Mei beta ner Influence of PEG Chain Length of Functionalized Magnetic Nanoparticles on the Cytocompatibility and Immune Competence of Primary Murine Macrophages and Dendritic Cells International Journal of Molecular Sciences, 2023-01-29;24(3):. 2023-01-29 [PMID: 36768890] (Bioassay, Mouse) Bioassay Mouse
R Storjohann, B Gericke, J Reifenrath, T Herrmann, P Behrens, H Oltmanns, J Mei beta ner Influence of PEG Chain Length of Functionalized Magnetic Nanoparticles on the Cytocompatibility and Immune Competence of Primary Murine Macrophages and Dendritic Cells International Journal of Molecular Sciences, 2023;24(3):. 2023 [PMID: 36768890] (Bioassay, Mouse) Bioassay Mouse
Z Su, J Brooks, J Pelker, T Andreyeva, H Sobon, R Gifford, M Powers, J Wang, C Dower, M Hegen, D Messing, AD Sheehan, JJ Brennan Studies with neutralizing antibodies suggest CXCL8-mediated neutrophil activation is independent of C-C motif chemokine receptor-like 2 (CCRL2) ligand binding function PLoS ONE, 2023-01-20;18(1):e0280590. 2023-01-20 [PMID: 36662882] (Bioassay, Human) Bioassay Human
J Liu, Y Cheng, X Zhang, Y Chen, H Zhu, K Chen, S Liu, Z Li, X Cao Glycosyltransferase Extl1 promotes CCR7-mediated dendritic cell migration to restrain infection and autoimmunity Cell Reports, 2023-01-18;42(1):111991. 2023-01-18 [PMID: 36656709] (Bioassay, Mouse) Bioassay Mouse
EW Wolf, ZP Howard, L Duan, H Tam, Y Xu, JG Cyster GPR174 signals via Galphas to control a CD86-containing gene expression program in B cells Proceedings of the National Academy of Sciences of the United States of America, 2022-05-31;119(23):e2201794119. 2022-05-31 [PMID: 35639700] (Bioassay, Mouse) Bioassay Mouse
W Piao, L Li, V Saxena, J Iyyathurai, R Lakhan, Y Zhang, IT Lape, C Paluskievi, KL Hippen, Y Lee, E Silverman, MW Shirkey, LV Riella, BR Blazar, JS Bromberg PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration Nature Communications, 2022-04-21;13(1):2176. 2022-04-21 [PMID: 35449134] (Bioassay, Mouse) Bioassay Mouse
V Saxena, W Piao, L Li, C Paluskievi, Y Xiong, T Simon, R Lakhan, CC Brinkman, S Walden, KL Hippen, M WillsonShi, YS Lee, C Wagner, BR Blazar, JS Bromberg Treg tissue stability depends on lymphotoxin beta-receptor- and adenosine-receptor-driven lymphatic endothelial cell responses Cell Reports, 2022-04-19;39(3):110727. 2022-04-19 [PMID: 35443187] (Bioassay, Mouse) Bioassay Mouse
T Nakatani, K Tsujimoto, J Park, T Jo, T Kimura, Y Hayama, H Konaka, T Morita, Y Kato, M Nishide, S Koyama, S Nada, M Okada, H Takamatsu, A Kumanogoh The lysosomal Ragulator complex plays an essential role in leukocyte trafficking by activating myosin II Nature Communications, 2021-06-07;12(1):3333. 2021-06-07 [PMID: 34099704] (Bioassay, Mouse) Bioassay Mouse
X Niu, H Sang, J Wang Naringenin Attenuates Experimental Autoimmune Encephalomyelitis by Protecting the Intact of Blood-Brain Barrier and Controlling Inflammatory Cell Migration J Nutr Biochem, 2020-11-26;0(0):108560. 2020-11-26 [PMID: 33249188] (Bioassay, Mouse) Bioassay Mouse
Show All 39 Publications.

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Bioinformatics

Gene Symbol Ccl19
Uniprot