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CXCL12/SDF-1 Antibody (MM0211-9N26) [DyLight 488]

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Product Details

Summary
Reactivity Hu, Rt, ShSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, B/N, CyTOF-ready
Clone
MM0211-9N26
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-12221AF350
Alexa Fluor 405
NBP2-12221AF405
Alexa Fluor 488
NBP2-12221AF488
Alexa Fluor 532
NBP2-12221AF532
Alexa Fluor 594
NBP2-12221AF594
Alexa Fluor 647
NBP2-12221AF647
Alexa Fluor 700
NBP2-12221AF700
Alexa Fluor 750
NBP2-12221AF750
Allophycocyanin
NBP2-12221APC
Allophycocyanin/Cy7
NBP2-12221APCCY7
Biotin
NBP2-12221B
CoraFluor 1
NBP2-12221CL1
DyLight 350
NBP2-12221UV
DyLight 405
NBP2-12221V
DyLight 550
NBP2-12221R
DyLight 594
NBP2-12221DL594
DyLight 650
NBP2-12221C
DyLight 680
NBP2-12221FR
DyLight 755
NBP2-12221IR
FITC
NBP2-12221F
HRP
NBP2-12221H
Janelia Fluor 525
NBP2-12221JF525
Janelia Fluor 549
NBP2-12221JF549
Janelia Fluor 585
NBP2-12221JF585
Janelia Fluor 635
NBP2-12221JF635
Janelia Fluor 646
NBP2-12221JF646
Janelia Fluor 669
NBP2-12221JF669
PE
NBP2-12221PE
PE/Atto594
NBP2-12221PEATT594
PE/Cy5.5
NBP2-12221PECY55
PE/Cy7
NBP2-12221PECY7
PerCP
NBP2-12221PCP
Unconjugated
NBP2-12221
mFluor Violet 450 SE
NBP2-12221MFV450
mFluor Violet 500 SE
NBP2-12221MFV500
mFluor Violet 610 SE
NBP2-12221MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-12221
Azide and BSA Free
NBP2-12221
Azide and BSA Free
NBP2-12221-0.025mg
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CXCL12/SDF-1 Antibody (MM0211-9N26) [DyLight 488] Summary

Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Immunogen
Human recombinant CXCL12/SDF-1
Specificity
Detects human CXCL12 (both 1-alpha and 1 beta).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CXCL12
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Block/Neutralize
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot

Reactivity Notes

Use in Rat reported in scientific literature (PMID:32197646). Sheep reactivity from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for CXCL12/SDF-1 Antibody (MM0211-9N26) [DyLight 488]

  • chemokine (C-X-C motif) ligand 12
  • C-X-C motif chemokine 12
  • CXCL12
  • hIRH
  • hSDF-1
  • Intercrine reduced in hepatomas
  • IRH
  • PBSF
  • PBSFSDF-1a
  • Pre-B cell growth-stimulating factor
  • SCYB12
  • SDF1
  • SDF-1
  • SDF1A
  • SDF1B
  • SDF1SDF-1b
  • stromal cell-derived factor 1
  • TLSF
  • TLSF-a
  • TLSF-b
  • TPAR1

Background

The C-X-C or alpha chemokine family is characterized by a pair of cysteine residues separated by a single amino acid and primarily functions as chemoattractants for neutrophils. The C-X-C family includes IL-8, NAP-2, MSGA and stromal cell-derived factor-1, or SDF-1. SDF-1 was originally described as a pre-B cell stimulatory factor, but has now been shown to function as a potent chemoattractant for T cells and monocytes, but not neutrophils. Receptors for the C-X-C family are G protein-coupled, seven-pass, transmembrane domain proteins which include IL-8RA, IL-8RB and fusin (also designated LESTR or CXCR-4). Fusin is highly homologous to the IL-8 receptors, sharing 37% sequence identity at the amino acid level. The IL-8 receptors bind to IL-8, NAP-2 and MSGA, while fusin binds to its cognate ligand, SDF-1. Fusin has been identified as the major co-receptor for T-tropic HIV-1, and SDF-1 has been shown to inhibit HIV-1 infection. Six human SDF-1 isoforms exist due to alternative splicing of CXCL12, the gene encoding SDF-1. Three isoforms are known for mouse and rat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CXCL12/SDF-1 Antibody (NBP2-12221G). (Showing 1 - 1 of 1 FAQ).

  1. I'm interested in your SDF1 antibody (MM0211-9N26) with a DyLight 488 conjugate but want to know if you do it in a smaller size than 100 tests as we're not sure that it will answer the question that we want to ask about SDF-1 attachment to HUVEC endothelial cells. Is it possible that you could supply a smaller size so that we could test to see if it is able to pick up the SDF-1 molecule on the surface of HUVEC cells?
    • Thank you also for your interest in our SDF1 antibody NBP2-12221G. Unfortunately due to our conjugation process, we are unable to provide sizes smaller than 100 tests at this time. We also only have one size available for the base unconjugated product (NBP2-12221). I am sorry for any inconvenience.

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Bioinformatics

Gene Symbol CXCL12