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CISH/CIS-1 Antibody [Unconjugated]

 (2 Publications)
 (1 Review)
 
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Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CIS-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3194) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CIS-1 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CIS-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3194) ...read more
CIS‑1 was detected in immersion fixed HDLM‑2 human Hodgkin’s lymphoma cell line using Goat Anti-Human CIS‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3194) at 15 µg/mL ...read more
Simple Western lane view shows lysates of NK human natural killer lymphoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for CIS‑1 at approximately 37 and 42 kDa (as indicated) using 10 µg/mL of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF3194-SP
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CISH/CIS-1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CIS-1
Leu11-Leu258
Accession # Q9NSE2
Specificity
Detects human CIS-1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CISH
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 1
using
AF3194 in the following applications:
Publications
Read Publications using
AF3194 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CISH/CIS-1 Antibody [Unconjugated]

  • CIS-1
  • CIS-1cytokine-inducible SH2-containing protein
  • CIScytokine-inducible inhibitor of signaling type 1B
  • CISH
  • cytokine inducible SH2-containing protein
  • G18Protein G18
  • SOCS
  • Suppressor of cytokine signaling

Background

Cytokine Inducible SH2-containing protein (CIS-1) is a 29 kDa protein found in a variety of cell types. Mono or polyubiquitination generally results in a 37 or 45 kDa molecule. CIS-1 binds to phosphorylated cytokine receptors IL-3 R beta and EPO-R and blocks downstream activation of STAT5 via receptor internalization and ubiquitin‑mediated proteosomal degradation. Human CIS-1 is a 258 aa peptide that contains one SH2 domain (aa 82‑163) and one SOCS box (aa 218‑258). There are two known alternatively spliced variants with a 7- or 13-aa substitution for the 7 N-terminal amino acid residues. Over the region used as immunogen, human CIS-1 is 91% identical to the corresponding mouse and canine protein sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CISH/CIS-1 Antibody (AF3194)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Western Blot
(1)
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(1)
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Review for CISH/CIS-1 Antibody (AF3194) (1) 11

Average Rating: 1
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF3194:
Filter by Applications
Simple Western
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  1
reviewed by:
Maud TUSSEAU
 Simple Western Human 08/06/2021
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Summary

ApplicationSimple Western
Sample TestedHek 293T cell lysate
SpeciesHuman
LotWON032106A

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.

Product General Protocols

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FAQs for CISH/CIS-1 Antibody (AF3194) (0)

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Secondary Antibodies

 

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Simple Western CISH/CIS-1 AF3194
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 Maud TUSSEAU
08/06/2021
Application: Simple Western
Species: Human
read this review

Bioinformatics

Gene Symbol CISH
Uniprot