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Brg1 Antibody [Unconjugated]

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 (1 Review)
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line, cytoplasmic and nuclear extracts. PVDF membrane was probed with 2 µg/mL Goat Anti-Human Brg1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5738) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Brg1 was detected at approximately 205 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line, cytoplasmic and nuclear extracts. PVDF membrane was probed with 2 µg/mL Goat ...read more
Brg1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Brg1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5738) at 10 µg/mL for 3 hours at room ...read more
Brg1 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human Brg1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5738) at 10 µg/mL for 3 hours at room temperature. ...read more
Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Brg1 knockout HEK293T cell line (KO). PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Brg1 Antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5738-SP
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Brg1 Antibody [Unconjugated] Summary

Specificity
Detects human Brg1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SMARCA4
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Knockout Validated
  • Western Blot 2 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF5738 in the following applications:
Publications
Read Publications using AF5738.

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Brg1 Antibody [Unconjugated]

  • ATP-dependent helicase SMARCA4
  • BAF190
  • BAF190A
  • brahma protein-like 1
  • Brg1
  • BRG1-associated factor 190A
  • BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4
  • BRM/SWI2-related gene 1
  • EC 3.6.1
  • EC 3.6.4.-
  • FLJ39786
  • global transcription activator homologous sequence
  • homeotic gene regulator
  • hSNF2b
  • Mitotic growth and transcription activator
  • nuclear protein GRB1
  • Protein brahma homolog 1
  • Protein BRG-1
  • RTPS2
  • SMARCA4
  • SNF2
  • SNF2B
  • SNF2-beta
  • SNF2L4
  • SNF2LB
  • SNF2-like 4
  • subfamily a, member 4
  • sucrose nonfermenting-like 4
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin
  • SWI2
  • transcription activator BRG1

Background

Brg1 (Brm/Swi2-related gene 1), also known as SMARCA4 (Swi/SNF-related matrix-associated actin-dependent regulator of chromatin, subfamily A, member 4), SNF2L4 and SNF2 beta , is a 205 kDa member of the SNF2 helicase family of molecules. It is a ubiquitously expressed, nuclear-localized chromatin remodeling ATPase that, depending upon its associated complex, may both facilitate and inhibit gene transcription. Human SMARCA4 is 1647 amino acids (aa) in length, and contains an HSA domain (aa 460‑532), one BRK region (aa 610‑654), six potential phosphoserine sites between aa 610‑662, a DEXDc domain that unwinds RNA and DNA (aa 774‑913), one Bromo domain (aa 1477‑1547) and eight potential phosphoserine sites between aa 1570‑1644.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Brg1 Antibody (AF5738)(3)


Review for Brg1 Antibody (AF5738) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF5738:
Filter by Applications
IF
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence Brg1 AF5738
Enlarge 		5
reviewed by:
Verified Customer
 IF Mouse 10/15/2015
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Summary

ApplicationImmunofluorescence
SpeciesMouse

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FAQs for Brg1 Antibody (AF5738) (0)

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Immunofluorescence Brg1 AF5738
Enlarge
 Verified Customer
10/15/2015
Application: IF
Species: Mouse
read this review

Bioinformatics

Gene Symbol SMARCA4
Uniprot