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BAF57 Antibody

Images

 
Western Blot: BAF57 Antibody [NBP1-90012] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: BAF57 Antibody [NBP1-90012] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Orthogonal Strategies: Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012] - Staining in human endometrium and liver tissues using anti-SMARCE1 antibody. Corresponding SMARCE1 RNA-seq data are presented ...read more
Western Blot: BAF57 Antibody [NBP1-90012] - Analysis in human cell line MOLT-4.
Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012] - Staining of human stomach shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012] - Staining of human endometrium shows high expression.
Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012] - Staining of human liver shows low expression as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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BAF57 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EEREKEAAEQAERSQSSIVPEEEQAANKGEEKKDDENIPMETEETHLEETTESQQNGEEGTSTPEDKESGQEGVDSMAEEGTSDSNTGSESNSATVEEPPTDPIPEDEK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMARCE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
BAF57 Protein (NBP1-90012PEP)
Publications
Read Publications using NBP1-90012.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for BAF57 Antibody

  • BAF57BRG1-associated factor 57
  • chromatin remodeling complex BRG1-associated factor 57
  • subfamily e, member 1
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin
  • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1
  • SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily E member 1

Background

BAF57 is encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BAF57 Antibody (NBP1-90012)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BAF57 Antibody (NBP1-90012). (Showing 1 - 1 of 1 FAQs).

  1. Can you tell me how baf 57 stains in normal breast tissue? and provide reference/pictures?
    • You can find images using this antibody to stain normal breast tissue here on Human Protein Atlas as this direct antibody was used for those staining images (https://www.proteinatlas.org/ENSG00000073584-SMARCE1/tissue/breast).

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMARCE1
Entrez
Uniprot