Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. Gels were loaded with 30 µg of cytoplasmic (Cyto) and 15 µg of nuclear extracts ...read more
Brg1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Rabbit Anti-Human Brg1 Monoclonal Antibody (Catalog # MAB5738) at 1 µg/mL for 1 hour at room temperature ...read more
Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Brg1 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Brg1 ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Brg1 Antibody (2135A) [Unconjugated]
ATP-dependent helicase SMARCA4
BAF190
BAF190A
brahma protein-like 1
Brg1
BRG1-associated factor 190A
BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4
BRM/SWI2-related gene 1
EC 3.6.1
EC 3.6.4.-
FLJ39786
global transcription activator homologous sequence
homeotic gene regulator
hSNF2b
Mitotic growth and transcription activator
nuclear protein GRB1
Protein brahma homolog 1
Protein BRG-1
RTPS2
SMARCA4
SNF2
SNF2B
SNF2-beta
SNF2L4
SNF2LB
SNF2-like 4
subfamily a, member 4
sucrose nonfermenting-like 4
SWI/SNF related, matrix associated, actin dependent regulator of chromatin
SWI2
transcription activator BRG1
Background
Brg1 (Brm/Swi2-related gene 1), also known as SMARCA4 (Swi/SNF-related matrix-associated actin-dependent regulator of chromatin, subfamily A, member 4), SNF2L4 and SNF2 beta , is a 205 kDa member of the SNF2 helicase family of molecules. It is a ubiquitously expressed, nuclear-localized chromatin remodeling ATPase that, depending upon its associated complex, may both facilitate and inhibit gene transcription. Human SMARCA4 is 1647 amino acids (aa) in length, and contains an HSA domain (aa 460‑532), one BRK region (aa 610‑654), six potential phosphoserine sites between aa 610‑662, a DEXDc domain that unwinds RNA and DNA (aa 774‑913), one Bromo domain (aa 1477‑1547) and eight potential phosphoserine sites between aa 1570‑1644.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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