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BIN1 Antibody (99F) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, DB, ELISA, ICC/IF, IP
Clone
99F
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-21674AF350
Alexa Fluor 405
NBP2-21674AF405
Alexa Fluor 488
NBP2-21674AF488
Alexa Fluor 532
NBP2-21674AF532
Alexa Fluor 594
NBP2-21674AF594
Alexa Fluor 647
NBP2-21674AF647
Alexa Fluor 700
NBP2-21674AF700
Alexa Fluor 750
NBP2-21674AF750
Biotin
NBP2-21674B
DyLight 350
NBP2-21674UV
DyLight 405
NBP2-21674V
DyLight 488
NBP2-21674G
DyLight 550
NBP2-21674R
DyLight 594
NBP2-21674DL594
DyLight 650
NBP2-21674C
DyLight 680
NBP2-21674FR
DyLight 755
NBP2-21674IR
FITC
NBP2-21674F
HRP
NBP2-21674H
Janelia Fluor 525
NBP2-21674JF525
Janelia Fluor 549
NBP2-21674JF549
Janelia Fluor 585
NBP2-21674JF585
Janelia Fluor 635
NBP2-21674JF635
Janelia Fluor 646
NBP2-21674JF646
Janelia Fluor 669
NBP2-21674JF669
Unconjugated
NBP2-21674
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BIN1 Antibody (99F) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
BIN1 Antibody (99F) was produced in mouse by repeated immunizations with BIN1 polypeptide followed by hybridoma development. (Uniprot: O00499)
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
BIN1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Dot Blot
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Cross-reactivity with BIN1 from other sources has not been determined

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for BIN1 Antibody (99F) [CoraFluor™ 1]

  • AMPH2
  • Amphiphysin II
  • Amphiphysin-like protein
  • AMPHLDKFZp547F068
  • box dependant MYC interacting protein 1
  • bridging integrator 1Box-dependent myc-interacting protein 1
  • MGC10367
  • myc box-dependent-interacting protein 1
  • SH3P9

Background

BIN1 encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BIN1