![Western Blot: Myotubularin Antibody [NB300-949] - Staining of Mouse Skeletal Muscle lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Myotubularin-Antibody-Western-Blot-NB300-949-img0008.jpg "Western Blot: Myotubularin Antibody [NB300-949] - Staining of Mouse Skeletal Muscle lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-SSPSQMMPHVQTHF corresponding to C-Terminus according to NP_000243.1. |
| Predicted Species | Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | MTM1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx. 70 kDa band observed in lysates of human skeletal muscle, mouse heart and skeletal muscle and HepG2 (calculated MW of 69.9 kDa band according to NP_000243.1and 69.6 kDa band according to mouse NP_001157662.1). |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NB300-949 | Applications | Species |
|---|---|---|
| Laporte J, Hu LJ, Kretz C et al. A gene mutated in X-linked myotubular myopathy defines a new putative tyrosine phosphatase family conserved in yeast. Nat Genet 1996-06-01 [PMID: 8640223] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Myotubularin Antibody (NB300-949)Find related products by research area.
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| Gene Symbol | MTM1 |
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| Uniprot |
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![Immunohistochemistry-Paraffin: Myotubularin Antibody [NB300-949] - Staining of paraffin embedded Human Uterus. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Myotubularin-Antibody-Immunohistochemistry-Paraffin-NB300-949-img0009.jpg "Immunohistochemistry-Paraffin: Myotubularin Antibody [NB300-949] - Staining of paraffin embedded Human Uterus. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: Myotubularin Antibody [NB300-949] - Staining (0.3ug/ml) of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Myotubularin-Antibody-Western-Blot-NB300-949-img0005.jpg "Western Blot: Myotubularin Antibody [NB300-949] - Staining (0.3ug/ml) of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.")
![Western Blot: Myotubularin Antibody [NB300-949] - Staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Myotubularin-Antibody-Western-Blot-NB300-949-img0006.jpg "Western Blot: Myotubularin Antibody [NB300-949] - Staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Western Blot: Myotubularin Antibody [NB300-949] - Staining of Mouse Heart (A) and Skeletal Muscle (B) lysates (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Myotubularin-Antibody-Western-Blot-NB300-949-img0007.jpg "Western Blot: Myotubularin Antibody [NB300-949] - Staining of Mouse Heart (A) and Skeletal Muscle (B) lysates (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Western Blot: Myotubularin Antibody [NB300-949] - HEK293 overexpressing MTM1 and probed with (mock transfection in first lane).](http://images.novusbio.com/fullsize/Myotubularin-Antibody-Western-Blot-NB300-949-img0003.jpg "Western Blot: Myotubularin Antibody [NB300-949] - HEK293 overexpressing MTM1 and probed with (mock transfection in first lane).")
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