![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Analysis in human colon and liver tissues. Corresponding Dynamin 2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0009.jpg "Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Analysis in human colon and liver tissues. Corresponding Dynamin 2 RNA-seq data are presented for the same tissues.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: PPQIPSRPVRIPPGIPPGVPSRR |
| Predicted Species | Mouse (96%), Rat (96%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DNM2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Knockout validation (PMID: 31390291). |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
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| Publications using NBP2-47477 | Applications | Species |
|---|---|---|
| Engevik AC, Kaji I, Postema MM et al. Loss of myosin Vb promotes apical bulk endocytosis in neonatal enterocytes J. Cell Biol. 2019-09-27 [PMID: 31562230] (WB) | WB | |
| Postema MM Mechanisms of BAR Protein Function in Brush Border Assembly Thesis 2019-09-30 (ICC/IF) | ICC/IF | |
| Postema MM, Grega-Larson NE, Meenderink LM, Tyska MJ PACSIN2-dependent apical endocytosis regulates the morphology of epithelial microvilli Mol. Biol. Cell 2019-09-01 [PMID: 31390291] (ICC/IF, KO, Human) | ICC/IF, KO | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Dynamin 2 Antibody (NBP2-47477)Find related products by research area.
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EEA1 Antibodies in Endosomal Transport and Signalling Studies EEA1, or Early Endosome Antigen 1, is a phospholipid-binding protein essential for endosomal membrane trafficking and fusion, and is a useful endosomal marker. We at Novus Biologicals have an extensive EEA1 antibody catalog, with antibodies that have ... Read full blog post. |
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| Gene Symbol | DNM2 |
![Western Blot: Dynamin 2 Antibody [NBP2-47477] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Western-Blot-NBP2-47477-img0003.jpg "Western Blot: Dynamin 2 Antibody [NBP2-47477] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.")
![Immunocytochemistry/Immunofluorescence: Dynamin 2 Antibody [NBP2-47477] - Staining of human cell line HeLa shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47477-img0004.jpg "Immunocytochemistry/Immunofluorescence: Dynamin 2 Antibody [NBP2-47477] - Staining of human cell line HeLa shows localization to the Golgi apparatus. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0012.jpg "Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human colon shows high expression.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0006.jpg "Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human colon shows high expression.")
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0010.jpg "Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human skin shows weak to moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0011.jpg "Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human skin shows weak to moderate cytoplasmic positivity in squamous epithelial cells.")
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