Rab5a Antibody

Rab5 is a 24 kDa GTP-binding protein that regulates the fusion of plasma membrane -derived clathrin-coated vesicles with early endosomes , and homotypic fusion among early endosomes1. Localized to the cytoplasmic side of the plasma membrane, clathrin -coated vesicles and early endosomes, Rab5 appears to regulate vesicle fusion through a cycle of GDP/GTP e xchange and GTP hydrolysis. The different guanine nuc leotide binding states of Rab5 may affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. The GTP-bound, or active, form of Rab5 associates with membrane s and regulates vesicle docking and fusion. Studies using a Rab5 mutant that hydrolyzed xanthosine 5-triphosphate (XTP) indicated that nucleotide hydrolysis occurs even in the absence of membrane fusion. GTP hydrolysis by Rab5 may determine the frequency of membrane docking and fusion events.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Functional, ICC/IF, IP, WB.

Showing Publications 1 - 10 of 17. Show All 17 Publications. Collapse Publications.

Publications using NB120-13253	Applications	Species
Choi GE, Park JY, Park MR et al. Glucocorticoid Enhances Presenilin1-Dependent A? Production at Er's Mitochondrial-Associated Membrane by Downregulating Rer1 in Neuronal Cells Redox biology 2023-06-16 [PMID: 37494768]
Choi GE, Park JY, Park MR et al. Glucocorticoid Enhances Presenilin1-Dependent A? Production at Er's Mitochondrial-Associated Membrane by Downregulating Rer1 in Neuronal Cells Redox biology 2023-06-16 [PMID: 37494768] (Functional)	Functional
Chae CW, Yoon JH, Lim JR et al. TRIM16-mediated lysophagy suppresses high-gluocse-accumulated neuronal A? Autophagy 2023-06-25 [PMID: 37357416]
Chae, CW;Choi, GE;Jung, YH;Lim, JR;Cho, JH;Yoon, JH;Han, HJ; High glucose-mediated VPS26a downregulation dysregulates neuronal amyloid precursor protein processing and tau phosphorylation British journal of pharmacology [PMID: 35297035]
Zhou B, Zhang F, Zhang W et al. Propofol Improves Survival In A Murine Model of Sepsis Via Inhibiting Rab5a -Mediated Intracellular Trafficking of TLR4 Research Square 2021-05-07
Chae CW, Lee HJ, Choi GE et al. High glucose-mediated PICALM and mTORC1 modulate processing of amyloid precursor protein via endosomal abnormalities Br. J. Pharmacol. 2020-05-20 [PMID: 32436237]
Fred SM, Laukkanen L, Brunello CA et al. Pharmacologically diverse antidepressants facilitate TRKB receptor activation by disrupting its interaction with the endocytic adaptor complex AP-2 J. Biol. Chem. 2019-10-20 [PMID: 31631060] (ICC/IF, Mouse)	ICC/IF	Mouse
Pang J, Peng H, Wang S et al. Mitochondrial ALDH2 protects against lipopolysaccharide-induced myocardial contractile dysfunction by suppression of ER stress and autophagy Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2019-04-01 [PMID: 30946956] (WB, Mouse)	WB	Mouse
Hagiwara M, Komatsu T, Sugiura SS et al. POT1b regulates phagocytosis and NO production by modulating activity of the small GTPase Rab5. Biochem Biophys Res Commun. 2013-08-13 [PMID: 23954637] (WB, IP, Mouse)	WB, IP	Mouse
Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (Human) Details: A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.		Human
Ali BR et al. J Cell Sci 117: 6401-6412. 2004-01-01 [PMID: 15561774]
Stenmark H, Olkkonen VM. Genome Biol. 2: 3007.1-3007.7. [PMID: 11387043]
Zerial M, McBride H. Nat. Rev. Mol. Cell Biol. 2: 107-117. 2001-01-01 [PMID: 11252952] (WB)	WB
Woodman PG. Traffic 1: 695-701. 2000-01-01 [PMID: 11208157]
Takai Y, Sasaki T, Matozaki T. Small GTP-binding proteins. Physiol Rev;81(1):153-208. 2001-01-01 [PMID: 11152757]
Sonnichsen B et al. J. Cell Biol. 149: 901-913. 2000-01-01 [PMID: 10811830] (WB)	WB
Show All 17 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Verified Customer	WB	Human	12/15/2017	View Summary Application Western Blot Sample Tested Human primary Colon Cancer cell line,human primary hepatocytes and Hep G2 Species Human Lot 140819 Comments Comments human primary hepatocytes and Hep G2 cells Name: Anti-Rab5a antibody (NB120-13253) Catalog #: Anti-Rab5a antibody (NB120-13253) Lot Number: Anti-Rab5a antibody (NB120-13253, Lot # 140819) PO/Order Number: Click here to enter text.. WB Image Description (Please provide labels for all lanes): lane 1: human primary hepatocytes; lane 2: Hep G2 Sample Information:      Cell Line or Tissue: human primary hepatocytes, Hep G2      Species: human      Treatment: No treatment Lysate Preparation:      Date of lysate preparation: December 11, 2017      Lysis buffer used: 1X lysis buffer from Cell Signaling by adding PMSF Reducing agent: beta-mercaptoethanol, DTT If boiled (temperature/time): Yes Controls:      Positive Control: No      Negative Control: No      Loading Control (please attach additional images if applicable): No Protein Amount Loaded per lane: 20 ug Antibody Storage Conditions: -20℃ Electrophoresis: Gel Percentage: 15% Electrophoresis Conditions: Tris-Glycine-SDS at room temperature      Voltage: 120V      Time: 2 hours Membrane Transfer:      Method (Submersion/Semi-dry): wet transfer      Membrane Type (PVDF/Nitrocellulose): Nitrocellulose      Time: 2 hours      Voltage: 100V Blocking:      Blocking Solution: 5% milk in 1X TBST      Time: 1 hour at room temperature Primary Antibody:      Dilution: 1/1000      Diluent Buffer: 2.5% BSA      Incubation Time: overnight      Incubation Temperature: 4℃ Washing Conditions:      Wash Solution: 1X TBST      Time and Repetitions: 5 min each for 3 times Secondary Antibody Manufacturer and Catalog #: Promega, W401B, Lot # 0000187662 Secondary description: goat anti-rabbit secondary antibody      Dilution: 1/2000      Diluent Buffer: 3% milk      Incubation Time: 1 hour      Incubation Temperature: room temperature Detection Method: Detection: ECL (GE, cat # RPN2209, lot # 9838243) Procedure: Add equal volume of A and B, mix and apply on the membranes for 3-5 min before exposure Development Time: 20 seconds Molecular weight of band(s): ~ 23 kDa Experimental Concerns and Observations: A specific band around 23 kDa was observed
Enlarge	4 reviewed by: Liyong Zhang	IF	Mouse	08/19/2014	View Summary Application Immunofluorescence Species Mouse Lot 140110

Array NB120-13253

• Rab5a Antibodies
• Rab5a ELISA Kits
• Rab5a Lysate
• Rab5a Proteins