![Western Blot: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of partial recombinant human EEA1 protein (14kDa) with EEA1 antibody clone 6D4.1D4 at 0.5 ug mL.](http://images.novusbio.com/fullsize/EEA1-Antibody-6D4-1D4-Western-Blot-NBP2-36568-img0006.jpg "Western Blot: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of partial recombinant human EEA1 protein (14kDa) with EEA1 antibody clone 6D4.1D4 at 0.5 ug mL.")
| Reactivity | Hu, Mu, Rt, Po, Bt, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | 6D4.1D4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Partial recombinant human EEA1 protein (between amino acids 50-300). [UniProt Q15075] |
| Localization | Cytoplasm. Early endosome membrane. |
| Marker | Early Endosome Marker |
| Predicted Species | Rat (95%), Porcine (95%), Bat (93%), Primate (99%). Backed by our 100% Guarantee. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | EEA1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
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| Publications using NBP2-36568 | Applications | Species |
|---|---|---|
| Smits DJ, Dekker J, Schot R et al. CLEC16A interacts with retromer and TRIM27, and its loss impairs endosomal trafficking and neurodevelopment Human genetics 2022-12-20 [PMID: 36538041] (ICC/IF, Human) | ICC/IF | Human |
| Lu G, Li S, Zhou C et al. Tenuivirus uses a molecular bridge strategy to overcome insect midgut barriers for virus persistent transmission. bioRxiv 2018-08-31 (ICC/IF, Virus) | ICC/IF | Virus |
| Murakami R, Chiba Y, Nishi N et al. Immunoreactivity of receptor and transporters for lactate located in astrocytes and epithelial cells of choroid plexus of human brain Neurosci Lett 2020-11-16 [PMID: 33212210] (ICC/IF, Human) Details: Immunofluorescence analysis and immunohistochemical analysis of human brain samples. Western blot analysis performed in HeLa and HT-29. |
ICC/IF | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for EEA1 Antibody (NBP2-36568)Find related products by research area.
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Understanding EEA1's Role in Membrane Endosome Fusion EEA1, or Early Endosome Antigen 1 is a Rab5 effector essential for early endocytic membrane fusion. The EEA1 antibody is used in membrane trafficking and chaperone studies, and as an endosome marker. We at Novus Biologicals have a comprehensive antibo... Read full blog post. |
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EEA1 Antibodies in Endosomal Transport and Signalling Studies EEA1, or Early Endosome Antigen 1, is a phospholipid-binding protein essential for endosomal membrane trafficking and fusion, and is a useful endosomal marker. We at Novus Biologicals have an extensive EEA1 antibody catalog, with antibodies that have ... Read full blog post. |
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![Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of FFPE tissue section of human testes using mouse monoclonal EEA1 antibody (clone 6D4.1D4) at 10 ug/ml concentration. The interstitial cells depicted a punctate to granular cytoplasmic staining pattern for EEA1 protein.](http://images.novusbio.com/fullsize/EEA1-Antibody-6D4-1D4-Immunohistochemistry-Paraffin-NBP2-36568-img0004.jpg "Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of FFPE tissue section of human testes using mouse monoclonal EEA1 antibody (clone 6D4.1D4) at 10 ug/ml concentration. The interstitial cells depicted a punctate to granular cytoplasmic staining pattern for EEA1 protein.")
![Flow (Intracellular): EEA1 Antibody (6D4.1D4) [NBP2-36568] - Intracellular staining of human EEA1 in Flow cytometry using 2.5 ug of antibody per 1 million cells. Isotype control was mouse IgG1.](http://images.novusbio.com/fullsize/EEA1-Antibody-6D4-1D4-Flow-Intracellular-NBP2-36568-img0002.jpg "Flow (Intracellular): EEA1 Antibody (6D4.1D4) [NBP2-36568] - Intracellular staining of human EEA1 in Flow cytometry using 2.5 ug of antibody per 1 million cells. Isotype control was mouse IgG1.")
![Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of FFPE tissue section of mouse testes using mouse monoclonal EEA1 antibody (clone 6D4.1D4) at 10 ug/ml concentration. The interstitial cells depicted a punctate to granular cytoplasmic staining pattern for EEA1 protein with no signal in other cell types.](http://images.novusbio.com/fullsize/EEA1-Antibody-6D4-1D4-Immunohistochemistry-Paraffin-NBP2-36568-img0005.jpg "Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of FFPE tissue section of mouse testes using mouse monoclonal EEA1 antibody (clone 6D4.1D4) at 10 ug/ml concentration. The interstitial cells depicted a punctate to granular cytoplasmic staining pattern for EEA1 protein with no signal in other cell types.")
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