![Western Blot: Rab7a Antibody [NBP1-87174] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RAB7A antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Rab7a-Antibody-Western-Blot-NBP1-87174-img0016.jpg "Western Blot: Rab7a Antibody [NBP1-87174] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RAB7A antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: CVLVFDVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAK |
| Marker | Late Endosome Marker |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RAB7A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100. |
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| Theoretical MW | 23 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
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| Publications using NBP1-87174 | Applications | Species |
|---|---|---|
| Choi GE, Park JY, Park MR et al. Glucocorticoid Enhances Presenilin1-Dependent A? Production at Er's Mitochondrial-Associated Membrane by Downregulating Rer1 in Neuronal Cells Redox biology 2023-06-16 [PMID: 37494768] (Func) | Func | |
| Pera M, Larrea D, Guardia-Laguarta C et al. Increased localization of APP-C99 in mitochondria-associated ER membranes causes mitochondrial dysfunction in Alzheimer disease. EMBO J. 2017-10-10 [PMID: 29018038] (WB, Mouse) | WB | Mouse |
| Peinado JR, Chaplot K, Jarvela TS, Barbieri E Phase separation of proSAAS into spheres results in core sequestration of TDP-43216-414 aggregates bioRxiv 2020-01-01 |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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Enlarge |
reviewed by:
Verified Customer |
IF | Human | 07/10/2018 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for Rab7a Antibody (NBP1-87174)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Rab7a Antibody [NBP1-87174] - Staining of human cell line U-251MG shows positivity in vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Rab7a-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87174-img0003.jpg "Immunocytochemistry/Immunofluorescence: Rab7a Antibody [NBP1-87174] - Staining of human cell line U-251MG shows positivity in vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Rab7a-Antibody-Immunohistochemistry-Paraffin-NBP1-87174-img0015.jpg "Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.")
![Western Blot: Rab7a Antibody [NBP1-87174] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Rab7a-Antibody-Western-Blot-NBP1-87174-img0007.jpg "Western Blot: Rab7a Antibody [NBP1-87174] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Rab7a-Antibody-Immunohistochemistry-Paraffin-NBP1-87174-img0012.jpg "Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells.")
![Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human skeletal muscle shows moderate to strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Rab7a-Antibody-Immunohistochemistry-Paraffin-NBP1-87174-img0013.jpg "Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human skeletal muscle shows moderate to strong cytoplasmic positivity in myocytes.")
![Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Rab7a-Antibody-Immunohistochemistry-Paraffin-NBP1-87174-img0014.jpg "Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
