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PRICKLE1 Antibody - BSA Free

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Western Blot: PRICKLE1 Antibody - BSA Free [NBP2-81771] - Western blot analysis of PRICKLE1 in human bladder tissue lysate with PRICKLE1 antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: PRICKLE1 Antibody - BSA Free [NBP2-81771] - Immunofluorescence of PRICKLE1 in human bladder cells with PRICKLE1 antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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PRICKLE1 Antibody - BSA Free Summary

Immunogen
PRICKLE1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PRICKLE1. The immunogen is located within the last 50 amino acids of PRICKLE1. Amino Acid Squence: LPTPQFGQRTTKSKKKKGH
Specificity
Multiple isoforms of PRICKLE1 are known to exist. PRICKLE1 antibody is predicted to not cross-react with other PRICKLE protein family members.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRICKLE1
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Western Blot 1 ug/ml
Theoretical MW
52, 90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for PRICKLE1 Antibody - BSA Free

  • EPM1BMGC138902
  • FLJ31627
  • FLJ31937
  • prickle homolog 1 (Drosophila)
  • prickle-like 1 (Drosophila)
  • prickle-like 1
  • prickle-like protein 1
  • REST (RE-1 silencing transcription factor)/NRSF (neuron-restrictive silencerfactor)-interacting LIM domain protein
  • REST/NRSF-interacting LIM domain protein 1
  • RILPMGC138903

Background

PRICKLE1 has 831 amino acids and is approximately 94kDa. PRICKLE1 localizes to the nuclear membrane and is involved in the movement of REST, a transcription repressor. PRICKLE1 is implicated in pathways that regulate neural tube development and gastrulation. PRICKLE1 may also be a nuclear receptor that negatively regulates the Wnt/beta-catenin signaling pathway. Current research on PRICKLE1 is being performed in relation to several diseases and disorders including neural tube defects and progressive myoclonic epilepsy type 1B. PRICKLE1 has also been shown to interact with Dishevelled 1, Dishevelled 2, Dishevelled 3, VIP, and SMURF2 in the Wnt signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRICKLE1