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Frizzled-3 Antibody [Unconjugated]

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Frizzled-3 was detected in immersion fixed frozen sections of mouse embryo (E11) using Mouse Frizzled-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1001) at 15 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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Frizzled-3 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3
His23-Val157
Accession # Q61086
Specificity
Detects mouse Frizzled-3 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Frizzled-1, rmFrizzled-4, rmFrizzled-6, rmFrizzled-7, and rmFrizzled-8 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FZD3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF1001 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Frizzled-3 Antibody [Unconjugated]

  • frizzled (Drosophila) homolog 3
  • frizzled homolog 3 (Drosophila)
  • Frizzled3
  • Frizzled-3
  • Fz-3
  • FZD3
  • hFz3

Background

Frizzled-3 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Frizzled-3 Antibody (AF1001)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IHC, Neutralization, Western Blot.


Filter By Application
IHC
(1)
Neutralization
(2)
Western Blot
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
Rat
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF1001 Applications Species
Jun Yu, Yuanchu She, Lixin Yang, Mengru Zhuang, Peng Han, Jianhui Liu et al. The m6 A Readers YTHDF1 and YTHDF2 Synergistically Control Cerebellar Parallel Fiber Growth by Regulating Local Translation of the Key Wnt5a Signaling Components in Axons Advanced Science 11/1/2021 [PMID: 34643063]
B Feng, AE Freitas, L Gorodetski, J Wang, R Tian, YR Lee, AS Grewal, Y Zou Planar cell polarity signaling components are a direct target of beta-amyloid-associated degeneration of glutamatergic synapses Science Advances, 2021-08-18;7(34):. 2021-08-18 [PMID: 34407949] (IHC) IHC
Koshi Kunimoto, Roy D. Bayly, Eszter K. Vladar, Tyson Vonderfecht, Anna-Rachel Gallagher, Jeffrey D. Axelrod Disruption of Core Planar Cell Polarity Signaling Regulates Renal Tubule Morphogenesis but Is Not Cystogenic Current Biology 10/23/2017 [PMID: 29033332]
Xin Wang SNX27 Deletion Causes Hydrocephalus by Impairing Ependymal Cell Differentiation and Ciliogenesis J. Neurosci, 2016-12-14;36(50):12586-12597. 2016-12-14 [PMID: 27974614] (Western Blot, Mouse) Western Blot Mouse
Shi , Yuqiang, Shu , Jianhong, Gelman , Benjamin, Lisinicchia , Joshua G, Tang , Shao-Jun Wnt signaling in the pathogenesis of human HIV-associated pain syndromes. J Neuroimmune Pharmacol, 2013-06-05;8(4):956-64. 2013-06-05 [PMID: 23737037] (Western Blot, Human) Western Blot Human
Blakely BD, Bye CR, Fernando CV Wnt5a regulates midbrain dopaminergic axon growth and guidance. PLoS ONE, 2011-03-31;6(3):e18373. 2011-03-31 [PMID: 21483795] (Neutralization, Mouse, Rat) Neutralization Mouse, Rat
Annouck Luyten, Xuefeng Su, Sarah Gondela, Ying Chen, Santiago Rompani, Ayumi Takakura et al. Aberrant Regulation of Planar Cell Polarity in Polycystic Kidney Disease Journal of the American Society of Nephrology 9/1/2010 [PMID: 20705705]
Endo Y, Beauchamp E, Woods D, Taylor WG, Toretsky JA, Uren A, Rubin JS Wnt-3a and Dickkopf-1 stimulate neurite outgrowth in Ewing tumor cells via a Frizzled3- and c-Jun N-terminal kinase-dependent mechanism. Mol. Cell. Biol., 2008-01-22;28(7):2368-79. 2008-01-22 [PMID: 18212053] (Neutralization, Human) Neutralization Human

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Bioinformatics

Gene Symbol FZD3
Uniprot