Frizzled-4 Antibody (145901)

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Frizzled-4, designated CD344, is a 7-transmembrane glycoprotein of the Frizzled family within the G-protein coupled receptor superfamily (1, 2). Frizzled proteins function as receptors for Wnt proteins and can activate canonical Wnt/beta-catenin signaling as well as planar cell polarity and calcium flux pathways (1). Frizzled-4 is particularly important in angiogenic Wnt pathway signaling (1, 5). Frizzleds contain a divergent N-terminal signal peptide, a highly conserved ~120  amino acid (aa) cysteine-rich domain (CRD), a variable length linker region, seven transmembrane domains, and a variable-length C-terminal tail (1). The mouse Frizzled-4 cDNA encodes 537 aa with a 36 aa signal sequence and a 186 aa N-terminal extracellular sequence (4). The portion expressed includes aa 37-180, and shares 93%, 94%, 90%, 89% and 88% identity with human, rat, equine, bovine and canine Frizzled-4, respectively. This portion competes for Wnt binding with endogenous receptors. In humans, a 122 aa soluble form that diverges at aa 95 is proposed to be a positive regulator of Wnt signaling pathways (5). Frizzled-4 is unusual in binding a non-wnt ligand, Norrin, in addition to binding Wnt ligands (1, 3, 6). Norrin binds the Frizzled-4 CRD, activates Wnt signaling pathways and uses LRP5/6 as co-receptors (3, 6). Deletion of either Frizzled-4 or Norrin in mice results in a similar phenotype including malformation of vasculature in the retina, cerebellar degeneration, and loss of hair cells in the inner ear (1, 3, 7). In humans, blindness due to familial exudative vitreoretinopathy (FEVR) is associated with mutations producing loss of function of Frizzled-4 or Norrin, designated EVR1 and EVR2, respectively (1, 3, 8). Frizzled-4 expression has been found in many tissues, including mouse ovary, where it influences corpus luteum vasculogenesis and is necessary for fertility (4, 9).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 4 applications: Flow Cytometry, ICC, IHC-P, Western Blot.

Showing Publications 1 - 7 of 7.

Publications using MAB194	Applications	Species
Ando, T;Takazawa, I;Spencer, ZT;Ito, R;Tomimori, Y;Mikulski, Z;Matsumoto, K;Ishitani, T;Denson, LA;Kawakami, Y;Kawakami, Y;Kitaura, J;Ahmed, Y;Kawakami, T; Ileal Crohn's Disease Exhibits Reduced Activity of Phospholipase C-?3-Dependent Wnt/?-Catenin Signaling Pathway Cells 2024-06-05 [PMID: 38891118] (Western Blot, Mouse, Transgenic Mouse)	Western Blot	Mouse, Transgenic Mouse
Shu, Z;Fan, M;Tu, B;Tang, Z;Wang, H;Li, H;Li, H;Yuan, M;Bai, J;Huo, S;Wang, L;Zhu, WG;Wang, W;Liu, X;Shu, S;Zhao, Y; The Lin28b/Wnt5a axis drives pancreas cancer through crosstalk between cancer associated fibroblasts and tumor epithelium Nature communications 2023-10-28 [PMID: 37898598] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
Aries, A;Zanetti, C;Hénon, P;Drénou, B;Lahlil, R; Deciphering the Cardiovascular Potential of Human CD34+ Stem Cells International journal of molecular sciences 2023-05-31 [PMID: 37298503] (ICC, Human)	ICC	Human
W Liu, L Zhang, K Xuan, C Hu, L Li, Y Zhang, F Jin, Y Jin Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3? Complex in Hypophosphatasia Theranostics, 2018-11-09;8(20):5575-5592. 2018-11-09 [PMID: 30555565] (Western Blot, Human)	Western Blot	Human
Felicitas Bucher, Ding Zhang, Edith Aguilar, Susumu Sakimoto, Sophia Diaz-Aguilar, Mauricio Rosenfeld et al. Antibody-Mediated Inhibition of Tspan12 Ameliorates Vasoproliferative Retinopathy Through Suppression of beta -Catenin Signaling Circulation 7/11/2017 [PMID: 28356444]
Baksh D, Boland GM, Tuan RS Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation. J. Cell. Biochem., 2007-08-01;101(5):1109-24. 2007-08-01 [PMID: 17546602] (Flow Cytometry, Human)	Flow Cytometry	Human
Chen J, Park CS, Tang SJ Activity-dependent synaptic Wnt release regulates hippocampal long term potentiation. J. Biol. Chem., 2006-02-24;281(17):11910-6. 2006-02-24 [PMID: 16501258] (IHC-P, Mouse)	IHC-P	Mouse

Images	Ratings	Applications	Species	Date	Details
Enlarge	3 reviewed by: Verified Customer	WB	Mouse	06/28/2015	View Summary Application Western Blot Sample Tested mouse ear mesenchymal stem cell Species Mouse

Array MAB194

• Frizzled-4 Antibodies
• Frizzled-4 Lysate
• Frizzled-4 Protein
• Frizzled-4 Proteins and Enzymes