Rhodopsin Antibody (4D2)

Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4).Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Zebrafish.

We have publications tested in 3 applications: ELISA, IF/IHC, IHC-Fr.

Showing Publications 1 - 6 of 6.

Publications using NBP2-59690	Applications	Species
Stemerdink M, Broekman S, Peters T et al. Generation and Characterization of a Zebrafish Model for ADGRV1-Associated Retinal Dysfunction Using CRISPR/Cas9 Genome Editing Technology Cells 2023-06-10 [PMID: 37371069] (Zebrafish)		Zebrafish
Schellens R, Broekman S, Peters T et al. A protein domain-oriented approach to expand the opportunities of therapeutic exon skipping for USH2A-associated retinitis pigmentosa Molecular Therapy - Nucleic Acids 2023-05-01 [PMID: 37313440] (IHC-Fr, Zebrafish)	IHC-Fr	Zebrafish
Attia K Roles of PIKfyve in the Retina: A Zebrafish Study Thesis 2023-01-01
Reurink J, de Vrieze E, Li CHZ et al. Scrutinizing pathogenicity of the USH2A c.2276G > T; p.(Cys759Phe) variant NPJ genomic medicine 2022-06-07 [PMID: 35672333] (IF/IHC, Zebrafish)	IF/IHC	Zebrafish
Kamel, SM;Broekman, S;Tessadori, F;van Wijk, E;Bakkers, J; The zebrafish cohesin protein Sgo1 is required for cardiac function and eye development Developmental dynamics : an official publication of the American Association of Anatomists [PMID: 35275424] (Human)		Human
Petersen ERB, Olsen DA, Christensen H et al. Rhodopsin in plasma from patients with diabetic retinopathy - development and validation of digital ELISA by Single Molecule Array (Simoa) technology. J. Immunol. Methods. 2017-04-05 [PMID: 28390926] (ELISA)	ELISA

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Sanne Broekman	IHC-Fr	Zebrafish	08/29/2019	View Summary Application Immunohistochemistry-Frozen Sample Tested Retina (outer nuclear layer) Species Zebrafish Lot MR187076 Comments Comments o/n PFA and Methanol fixed 7um sections Put slides in 0.1% PBS-Tween 20 1-2 minutes In 10mM sodium citrate pH 8.5 autoclave (1 min at 121°C, door open at 99⁰C ) Put back in 0.1% PBS-Tween20 Wash 3 x 5min 0.1% PBS-Tween20 1 hour block solution (10% NFDM in 0.1% PBS-Tween20) Overnight with Primary antibody 1:500- 1:5000 in block solution Wash 3 x 10 min 0.1% PBS-Tween20 2 hour incubation with Secondary antibody (1:500) + DAPI (1:10000) in block solution Wash 3 X 10 min 0.1% PBS-Tween20 Enclose with prolong gold antifade Store in dark at 4°C. ***Novus Innovators Program - new species or application used on a primary antibody.

Array NBP2-59690

• Rhodopsin Antibodies
• Rhodopsin ELISA Kits
• Rhodopsin Lysate
• Rhodopsin Protein