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Frizzled-4 Antibody (145901) [DyLight 680]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clone
145901
Clonality
Monoclonal
Host
Rat
Conjugate
DyLight 680

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Frizzled-4 Antibody (145901) [DyLight 680] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4
Phe37-Glu180
Accession # Q61088
Specificity
Detects human and mouse Frizzled-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) Frizzled-1, -2, -3, -6, -7, -8, or recombinant human Frizzled-5 is observed. Detects Frizzled-4 by flow cytometry and immunohistochemistry on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
FZD4
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Frizzled-4 Antibody (145901) [DyLight 680]

  • CD344 antigen
  • CD344
  • EVR1
  • exudative vitreoretinopathy 1
  • FEVR
  • frizzled (Drosophila) homolog 4
  • frizzled homolog 4 (Drosophila)
  • Frizzled4
  • Frizzled-4
  • Fz-4
  • FZD4
  • FZD4S
  • FzE4
  • GPCR
  • hFz4
  • MGC34390
  • WNT receptor frizzled-4

Background

FZD4 is a Frizzled Receptor involved in mediating Wnt signaling. A splicing variant of frizzled-4, FZD4s, encodes a soluble-type positive regulator of the Wnt signaling pathway. Mutations in FZD4 are associated with retinal angiogenesis. FZD4 is widely expressed, particularly in heart, skeletal muscle, ovary, and fetal kidney. Transcripts have also been identified in liver, kidney, pancreas, spleen, and fetal lung, and in smaller amounts in placenta, adult lung, prostate, testis, colon, and fetal brain. FZD4 expression has not been reported in several cancer cell lines studied. ESTs have been isolated from adipose, adrenal, B-cell/lung/testis, bone marrow, brain, breast, colon, embryo, gallbladder, head/neck, heart, heart/melanocyte/uterus, kidney, liver, liver/spleen, lung, placenta, prostate, skeletal muscle, skin, and vessel libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FZD4