Frizzled-4 Antibody (145901) [DyLight 650] Summary
Immunogen |
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4 Phe37-Glu180 Accession # Q61088 |
Specificity |
Detects human and mouse Frizzled-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) Frizzled-1, -2, -3, -6, -7, -8, or recombinant human Frizzled-5 is observed. Detects Frizzled-4 by flow cytometry and immunohistochemistry on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3.
|
Isotype |
IgG2a |
Clonality |
Monoclonal |
Host |
Rat |
Gene |
FZD4 |
Purity |
Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- CyTOF-ready
- Flow Cytometry
- Immunocytochemistry
- Western Blot
|
Application Notes |
Optimal dilution of this antibody should be experimentally determined. |
Packaging, Storage & Formulations
Storage |
Store at 4C in the dark. |
Buffer |
50mM Sodium Borate |
Preservative |
0.05% Sodium Azide |
Purity |
Protein A or G purified from hybridoma culture supernatant |
Notes
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Alternate Names for Frizzled-4 Antibody (145901) [DyLight 650]
Background
FZD4 is a Frizzled Receptor involved in mediating Wnt signaling. A splicing variant of frizzled-4, FZD4s, encodes a soluble-type positive regulator of the Wnt signaling pathway. Mutations in FZD4 are associated with retinal angiogenesis. FZD4 is widely expressed, particularly in heart, skeletal muscle, ovary, and fetal kidney. Transcripts have also been identified in liver, kidney, pancreas, spleen, and fetal lung, and in smaller amounts in placenta, adult lung, prostate, testis, colon, and fetal brain. FZD4 expression has not been reported in several cancer cell lines studied. ESTs have been isolated from adipose, adrenal, B-cell/lung/testis, bone marrow, brain, breast, colon, embryo, gallbladder, head/neck, heart, heart/melanocyte/uterus, kidney, liver, liver/spleen, lung, placenta, prostate, skeletal muscle, skin, and vessel libraries.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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