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PGPEP-1 Antibody

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Western blot shows lysates of LNCaP human prostate cancer cell line, JAR human choriocarcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and human brain ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

PGPEP-1 Antibody Summary

Immunogen
E. coli-derived recombinant human PGPEP-1
Met1-His209
Accession # Q9NXJ5
Specificity
Detects human PGPEP-1 in direct ELISAs and, human and mouse PGPEP-1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PGPEP1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PGPEP-1 Antibody

  • EC 3.4.19.3
  • PAP-I
  • Pcp
  • PGPEP1
  • PGPEP-1
  • PGPIFLJ20208
  • PGP-IPAP-I
  • PGPMGC10812
  • Pyroglutamyl aminopeptidase I
  • pyroglutamyl-peptidase 1
  • pyroglutamyl-peptidase I5-oxoprolyl-peptidase
  • Pyrrolidone-carboxylate peptidase

Background

Pyroglutamyl peptidase-1 (PGPEP-1) is an omega peptidase which removes pyroglutamyl residues from the amino termini of peptides and proteins (1). It is a cytosolic cysteine peptidase that is expressed in most cell types (2). The enzyme requires a thiol‑reducing agent for activity (3). PGPEP-1 is potentially involved in the inactivation of biologically active peptides that possess an amino terminal pyroglutamyl group (3). Examples of such peptides include neurotensin, luteinizing hormone releasing hormone, and thyrotropin-releasing hormone. Human and mouse PGPEP-1 share 95% aa sequence identity.
  1. Kilbane Z. et al. (2007) Mol. Cell. Biochem. 297:189.
  2. Cummins P.M. and B. O'Connor (1998) Biochim. Biophys. Acta. 1429:1.
  3. Dando P.M. et al. (2003) Protein Express. Purif. 28:111.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PGPEP1
Uniprot