![Western Blot: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis using CYP3A4 mAb against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Cytochrome-P450-3A4-Antibody-3H8-Western-Blot-NBP2-37502-img0003.jpg "Western Blot: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis using CYP3A4 mAb against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 3H8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Cytochrome P450 3A4 expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CYP3A4 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
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| Theoretical MW | 57.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
| Publication using NBP2-37502 | Applications | Species |
|---|---|---|
| Agarwal T, Rustagi A, Das J, Maiti TK. PAMAM dendrimer grafted cellulose paper scaffolds as a novel in vitro 3D liver model for drug screening applications. Colloids Surf B Biointerfaces 2018-08-29 [PMID: 30179804] (ICC, Human) | ICC | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytochrome P450 3A4 Antibody (NBP2-37502)Find related products by research area.
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| Gene Symbol | CYP3A4 |
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis of HepG2 cells using CYP3A4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Cytochrome-P450-3A4-Antibody-3H8-Immunocytochemistry-Immunofluorescence-NBP2-37502-img0004.jpg "Immunocytochemistry/Immunofluorescence: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis of HepG2 cells using CYP3A4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Immunohistochemistry-Paraffin: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis of human brain tissues using CYP3A4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytochrome-P450-3A4-Antibody-3H8-Immunohistochemistry-NBP2-37502-img0007.jpg "Immunohistochemistry-Paraffin: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis of human brain tissues using CYP3A4 mouse mAb with DAB staining.")
![Flow Cytometry: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis of HepG2 cells using CYP3A4 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Cytochrome-P450-3A4-Antibody-3H8-Flow-Cytometry-NBP2-37502-img0005.jpg "Flow Cytometry: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis of HepG2 cells using CYP3A4 mouse mAb (green) and negative control (red).")
![Western Blot: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis using CYP3A4 mAb against human CYP3A4 (AA: 243-430) recombinant protein. (Expected MW is 47.5 kDa)](http://images.novusbio.com/fullsize/Cytochrome-P450-3A4-Antibody-3H8-Western-Blot-NBP2-37502-img0002.jpg "Western Blot: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis using CYP3A4 mAb against human CYP3A4 (AA: 243-430) recombinant protein. (Expected MW is 47.5 kDa)")
![Immunohistochemistry-Paraffin: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis of liver cancer tissues using CYP3A4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytochrome-P450-3A4-Antibody-3H8-Immunohistochemistry-NBP2-37502-img0006.jpg "Immunohistochemistry-Paraffin: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis of liver cancer tissues using CYP3A4 mouse mAb with DAB staining.")
![ELISA: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Cytochrome-P450-3A4-Antibody-3H8-ELISA-NBP2-37502-img0001.jpg "ELISA: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")