![Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of COS-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-1A2-Antibody-MC1-Immunocytochemistry-Immunofluorescence-NB100-74398-img0003.jpg "Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of COS-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | MC1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | 3-methylcholanthrene induced rat cytochrome P450 protein. |
| Specificity | Detects Cytochrome P450 1A1 (Gene ID: 1543, UniProt P04798) and Cytochrom P450 1A2 (Gene ID: 1544, UniProt: P05177) from human samples. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CYP1A1 |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Detects an approx. 58 kDa protein. |
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| Theoretical MW | 58 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Ascites |
| Preservative | 0.05% Sodium Azide |
| Purity | Unpurified |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
WB | Human | 12/22/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cytochrome P450 1A1/1A2 Antibody (NB100-74398)Find related products by research area.
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| Gene Symbol | CYP1A1 |
![Immunohistochemistry-Paraffin: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis showing positive staining in the cytoplasm and membrane of Mouse liver tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-1A2-Antibody-MC1-Immunohistochemistry-Paraffin-NB100-74398-img0006.jpg "Immunohistochemistry-Paraffin: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis showing positive staining in the cytoplasm and membrane of Mouse liver tissue (right) compared with a negative control in the absence of primary antibody (left).")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescentred phalloidin and nuclei (blue) were stained with Hoechst or DAPI.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-1A2-Antibody-MC1-Immunocytochemistry-Immunofluorescence-NB100-74398-img0001.jpg "Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescentred phalloidin and nuclei (blue) were stained with Hoechst or DAPI.")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-1A2-Antibody-MC1-Immunocytochemistry-Immunofluorescence-NB100-74398-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.")
![Immunohistochemistry-Paraffin: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis showing staining in the cytoplasm and membrane of rat liver tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-1A2-Antibody-MC1-Immunohistochemistry-Paraffin-NB100-74398-img0004.jpg "Immunohistochemistry-Paraffin: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis showing staining in the cytoplasm and membrane of rat liver tissue (right) compared with a negative control without primary antibody (left).")
![Immunohistochemistry-Paraffin: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis showing positive staining in the membrane of Human prostate tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-1A2-Antibody-MC1-Immunohistochemistry-Paraffin-NB100-74398-img0005.jpg "Immunohistochemistry-Paraffin: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis showing positive staining in the membrane of Human prostate tissue (right) compared with a negative control in the absence of primary antibody (left).")
![Immunohistochemistry-Paraffin: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis showing positive staining in the cytoplasm and membrane of Human liver tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-1A2-Antibody-MC1-Immunohistochemistry-Paraffin-NB100-74398-img0007.jpg "Immunohistochemistry-Paraffin: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis showing positive staining in the cytoplasm and membrane of Human liver tissue (right) compared with a negative control in the absence of primary antibody (left).")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")