Novus Biologicals products are now on bio-techne.com

Donkey anti-Sheep IgG Secondary Antibody [HRP]

Images

 
Western blot shows detection of Sheep Anti-Human/Mouse/Rat PSMA1 Antigen Affinity-purified Polyclonal Antibody (AF7565) using a 1:1000 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). ...read more
Simple Western lane view shows detection of Sheep Anti-Mouse Contactin-6 Polyclonal Antibody (AF5890) using a 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was ...read more
Detection of AIF in human kidney using 3 ug/mL of Sheep anti-human AIF primary antibody (AF5824) followed by 30 min incubation with Donkey anti Sheep HRP conjugated secondary antibodies (Catalog # HAF016) at 1:20 ...read more

Product Details

Summary
Reactivity ShSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Donkey
Conjugate
HRP

Donkey anti-Sheep IgG Secondary Antibody [HRP] Summary

Immunogen
Sheep IgG
Specificity
Detects sheep IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with mouse IgG, rabbit IgG, human IgG, and chicken IgY is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Simple Western
  • Western Blot
Reviewed Applications
Read 2 Reviews rated 5
using
HAF016 in the following applications:

Publications
Read Publications using
HAF016 in the following applications:

Packaging, Storage & Formulations

Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Sodium Phosphate and Proclin 300.
Preservative
Proclin 300

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Sheep IgG Secondary Antibody [HRP]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-62076
Species: Hu
Applications: ELISA, ELISA(Cap), ELISA(Det)
NBP1-74190
Species: Mu
Applications: ChIP, WB
NBP2-79716
Species: Hu
Applications: IHC, IHC-P, PA
NB7276
Species: Ch
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NB200-540
Species: Ec, Mu
Applications: CyTOF-ready, Flow-IC, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-30955
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
H00728695-B01P
Species: Hu
Applications: ICC/IF, IP, WB
NBP1-88927
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-29373
Species: Hu, Mu, Rt
Applications: Flow-CS, Flow, ICC/IF
NBP2-43666
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-87404
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, KD, WB
NBP2-62025
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, ELISA(Cap), ELISA(Det)
DHAPG0
Species: Hu
Applications: ELISA
NBP1-43435
Species: Hu, Mu
Applications: Flow, WB
NBP2-19784
Species: Hu, Mu, Rt, Ye
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, PLA, WB
AF8537
Species: Mu, Rt
Applications: ICC, Simple Western, WB
AF1938
Species: Hu
Applications: ICC, IP, Simple Western, WB

Publications for IgG Antibody (HAF016)(82)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Bacteria - E. Coli, Cercopithecus aethiops, Gerbil, Ovine, Primate - Chlorocebus sabaeus (Green Monkey).

We have publications tested in 6 applications: IHC, IHC-Fr, IHC-P, Immunoprecipitation, Secondary Antibody, Western Blot.


Filter By Application
IHC
(2)
IHC-Fr
(2)
IHC-P
(1)
Immunoprecipitation
(1)
Secondary Antibody
(1)
Western Blot
(41)
All Applications
Filter By Species
Human
(25)
Mouse
(18)
Rat
(1)
Bacteria - E. Coli
(1)
Cercopithecus aethiops
(1)
Gerbil
(1)
Ovine
(1)
Primate - Chlorocebus sabaeus (Green Monkey)
(1)
All Species
Showing Publications 1 - 10 of 82. Show All 82 Publications.
Publications using HAF016 Applications Species
Suvarna, K;Jayabal, P;Ma, X;Wang, H;Chen, Y;Weintraub, ST;Han, X;Houghton, PJ;Shiio, Y; Ceramide-induced cleavage of GPR64 intracellular domain drives Ewing sarcoma Cell reports 2024-07-17 [PMID: 39024100] (Western Blot, Human) Western Blot Human
Kendsersky, NM;Odrobina, M;Mabe, NW;Farrel, A;Grossmann, L;Tsang, M;Groff, D;Wolpaw, AJ;Zammarchi, F;van Berkel, PH;Dang, CV;Mossé, YP;Stegmaier, K;Maris, JM; Lineage-dependence of the neuroblastoma surfaceome defines tumor cell state-dependent and independent immunotherapeutic targets bioRxiv : the preprint server for biology 2024-07-02 [PMID: 39005383] (Western Blot, Human) Western Blot Human
Simwela, NV;Johnston, L;Pavinski Bitar, P;Jaecklein, E;Altier, C;Sassetti, CM;Russell, DG; Genome-wide screen of Mycobacterium tuberculosis- infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth bioRxiv : the preprint server for biology 2024-05-08 [PMID: 38766174] (Western Blot, Mouse) Western Blot Mouse
Lu, L;Varshney, S;Yuan, Y;Wei, HX;Tanwar, A;Sundaram, S;Nauman, M;Haltiwanger, RS;Stanley, P; In vivo evidence for GDP-fucose transport in the absence of transporter SLC35C1 and putative transporter SLC35C2 The Journal of biological chemistry 2023-12-01 [PMID: 38270391] (Western Blot, Mouse) Western Blot Mouse
Shimizu, M;Shiraishi, N;Tada, S;Sasaki, T;Beck, G;Nagano, S;Kinoshita, M;Sumi, H;Sugimoto, T;Ishida, Y;Koda, T;Ishikura, T;Sugiyama, Y;Kihara, K;Kanakura, M;Nakajima, T;Takeda, S;Takahashi, MP;Yamashita, T;Okuno, T;Mochizuki, H; RGMa collapses the neuronal actin barrier against disease-implicated protein and exacerbates ALS Science advances 2023-11-24 [PMID: 37992159] (Western Blot, Mouse) Western Blot Mouse
VanDyke, D;Xu, L;Sargunas, PR;Gilbreth, RN;Baca, M;Gao, C;Hunt, J;Spangler, JB; Redirecting the specificity of tripartite motif containing-21 scaffolds using a novel discovery and design approach The Journal of biological chemistry 2023-10-20 [PMID: 37866632] (Western Blot, Human) Western Blot Human
Keary KM, Gu QH, Chen J, Li Z Dendritic distribution of autophagosomes underlies pathway-selective induction of LTD Cell reports 7/28/2023 [PMID: 37516958]

Details:
Dilutions: 1:500
X Shu, C Wei, WY Tu, K Zhong, S Qi, A Wang, L Bai, SX Zhang, B Luo, ZZ Xu, K Zhang, C Shen Negative regulation of TREM2-mediated C9orf72 poly-GA clearance by the NLRP3 inflammasome Cell Reports, 2023-02-16;42(2):112133. 2023-02-16 [PMID: 36800288] (Western Blot, Mouse) Western Blot Mouse
S Ganesan, NN Alvarez, S Steiner, KM Fowler, AK Corona, CR Roy Syntaxin 11 Contributes to the Interferon-Inducible Restriction of Coxiella burnetii Intracellular Infection MBio, 2023-02-02;0(0):e0354522. 2/2/2023 [PMID: 36728431]
E Duregotti, CM Reumiller, U Mayr, M Hasman, LE Schmidt, SA Burnap, K Theofilato, J Barallobre, A Beran, M Grandoch, A Viviano, M Jahangiri, M Mayr Reduced secretion of neuronal growth regulator 1 contributes to impaired adipose-neuronal crosstalk in obesity Nature Communications, 2022-11-25;13(1):7269. 2022-11-25 [PMID: 36433953] (Western Blot, Human, Mouse) Western Blot Human, Mouse
Show All 82 Publications.

Reviews for IgG Antibody (HAF016) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using HAF016:
Filter by Applications
ELISA
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
ELISA Human 08/12/2023
View

Summary

ApplicationELISA
Sample TestedHuman serum
SpeciesHuman
LotXDP1522051

Comments

CommentsELISA for human serum
  5
reviewed by:
Raminder Singh
WB Human 08/16/2021
View

Summary

ApplicationWestern Blot
Sample TestedC2BBe1 cell lysate
SpeciesHuman
LotXDP1319111

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IgG Antibody (HAF016) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgG Products

Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Annexin V Apoptosis Kit [FITC]
NBP2-29373-100Tests

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Verified Customer
08/12/2023
Application: ELISA
Species: Human

Raminder Singh
08/16/2021
Application: WB
Species: Human

Bioinformatics

Gene Symbol IGHG1