![Immunohistochemistry: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 followed by the Histar detection system. High power.](http://images.novusbio.com/fullsize/LRP-1-Antibody-A2Mr-alpha-2-Immunohistochemistry-NB100-64808-img0004.jpg "Immunohistochemistry: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 followed by the Histar detection system. High power.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, IP |
| Clone | A2Mr alpha-2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Additional Information | Clone A2Mr alpha-2 was used by HLDA to establish CD designation. |
| Immunogen | Purified alpha2 macroglobulin receptor |
| Specificity | Recognises an epitope in the extracellular region of the 500kD alpha chain. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | LRP1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Use non-reducing conditions for western blot. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for LRP-1 Antibody (NB100-64808)Find related products by research area.
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![Immunohistochemistry: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 followed by the Histar detection system. Low power.](http://images.novusbio.com/fullsize/LRP-1-Antibody-A2Mr-alpha-2-Immunohistochemistry-NB100-64808-img0001.jpg "Immunohistochemistry: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 followed by the Histar detection system. Low power.")
![Immunohistochemistry: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 followed by the Histar detection system. Medium power.](http://images.novusbio.com/fullsize/LRP-1-Antibody-A2Mr-alpha-2-Immunohistochemistry-NB100-64808-img0002.jpg "Immunohistochemistry: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 followed by the Histar detection system. Medium power.")
![Immunohistochemistry: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 followed by the Histar detection system. Medium power.](http://images.novusbio.com/fullsize/LRP-1-Antibody-A2Mr-alpha-2-Immunohistochemistry-NB100-64808-img0003.jpg "Immunohistochemistry: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 followed by the Histar detection system. Medium power.")
![Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2, red in A and Mouse anti Human CD21, clone LB21, green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power.](http://images.novusbio.com/fullsize/LRP-1-Antibody-A2Mr-alpha-2-Immunofluorescence-NB100-64808-img0005.jpg "Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2, red in A and Mouse anti Human CD21, clone LB21, green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power.")
![Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2, red in A and Mouse anti Human CD21, clone LB21, green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power.](http://images.novusbio.com/fullsize/LRP-1-Antibody-A2Mr-alpha-2-Immunofluorescence-NB100-64808-img0006.jpg "Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2, red in A and Mouse anti Human CD21, clone LB21, green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power.")
![Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2, red in A and Mouse anti Human CD21, clone LB21, green in B. C is the merged image with nuclei counterstained blue using DAPI. High power.](http://images.novusbio.com/fullsize/LRP-1-Antibody-A2Mr-alpha-2-Immunofluorescence-NB100-64808-img0007.jpg "Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2, red in A and Mouse anti Human CD21, clone LB21, green in B. C is the merged image with nuclei counterstained blue using DAPI. High power.")
![Immunocytochemistry/ Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Reduced EHD1 recruitment to endosomes upon EHD4 knock-down.A-F, Representative micrographs & insets depicting EHD1-GFP recruitment to endosomes in untreated (A & inset in D), mock-treated (B & inset in E), & EHD4 knock-down (C & inset in F) cells. CRISPR/Cas9 gene-edited NIH3T3 cells expressing endogenous levels of EHD1 with GFP fused to the C-terminus (EHD1-GFP) were either untreated, mock-treated with transfection reagent, or transfected with EHD4 siRNA for 72 h. Cells were then incubated with anti-LRP1 antibody (30 min on ice, 30 min at 37°C), fixed, & imaged via confocal microscopy. G, Immunoblot showing reduced EHD4 (but not EHD1-GFP) expression in EHD1-GFP cells, with actin used as a loading control. The nitrocellulose filter paper was then stripped & immunoblotted with anti-GFP to show EHD1-GFP expression upon EHD4 loss. H, Graph depicting the mean count of EHD1-labeled endosomes in untreated, mock-treated & EHD4-depleted cells. Individual experiments were performed 3 times. Error bars denote standard deviation & p-values were determined by one-way ANOVA for individual experiments using a post-hoc Tukey HSD calculator to determine significance. A consensus p-value was then derived as described in the Materials & methods to assess significant differences between samples from the 3 experiments. Micrographs are representative orthogonal projects from three independent experiments, with 10 sets of z-stacks collected for each treatment per experiment Bar, 10 μm. n.s., not significant (consensus p > 0.5). Consensus p-values from Tukey HSD: *p < 0.00001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32966336), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-64808_mouse-monoclonal-lrp-1-antibody-a2mr-alpha-2-310202415541917.jpg "Immunocytochemistry/ Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Reduced EHD1 recruitment to endosomes upon EHD4 knock-down.A-F, Representative micrographs & insets depicting EHD1-GFP recruitment to endosomes in untreated (A & inset in D), mock-treated (B & inset in E), & EHD4 knock-down (C & inset in F) cells. CRISPR/Cas9 gene-edited NIH3T3 cells expressing endogenous levels of EHD1 with GFP fused to the C-terminus (EHD1-GFP) were either untreated, mock-treated with transfection reagent, or transfected with EHD4 siRNA for 72 h. Cells were then incubated with anti-LRP1 antibody (30 min on ice, 30 min at 37°C), fixed, & imaged via confocal microscopy. G, Immunoblot showing reduced EHD4 (but not EHD1-GFP) expression in EHD1-GFP cells, with actin used as a loading control. The nitrocellulose filter paper was then stripped & immunoblotted with anti-GFP to show EHD1-GFP expression upon EHD4 loss. H, Graph depicting the mean count of EHD1-labeled endosomes in untreated, mock-treated & EHD4-depleted cells. Individual experiments were performed 3 times. Error bars denote standard deviation & p-values were determined by one-way ANOVA for individual experiments using a post-hoc Tukey HSD calculator to determine significance. A consensus p-value was then derived as described in the Materials & methods to assess significant differences between samples from the 3 experiments. Micrographs are representative orthogonal projects from three independent experiments, with 10 sets of z-stacks collected for each treatment per experiment Bar, 10 μm. n.s., not significant (consensus p > 0.5). Consensus p-values from Tukey HSD: *p < 0.00001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32966336), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Increased sorting endosome size & decreased EHD1 recruitment upon Rabenosyn-5 knock-down.A-L, Representative micrographs & insets depicting EEA1-labeled endosomes & EHD1-GFP in mock-treated & Rabenosyn-5 knock-down cells. CRISPR/Cas9 gene-edited NIH3T3 EHD1-GFP cells were either mock-treated with transfection reagent (A-F) or treated with Rabenosyn-5 siRNA (G-L) for 72 h. Cells were then incubated with anti-LRP1 antibody (30 min on ice, 30 min at 37°C), fixed & immunostained using anti-EEA1, & imaged by confocal microscopy. M, Immunoblot showing reduced Rabenosyn-5 expression in EHD1-GFP NIH3T3 cells. N, Graph depicting mean endosome size of mock-treated & Rabenosyn-5 knock-down cells. O, Graph depicting EHD1 recruitment to endosomes in mock-treated & Rabenosyn-5 knock-down cells. Error bars denote standard deviation & p-values were determined by independent two-tailed t-test, with significance derived from consensus p-values from the 3 experiments. Micrographs are representative orthogonal projections from three independent experiments, with 10 sets of z-stacks collected for each treatment per experiment. Bar, 10 μm. **p < 0.00001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32966336), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-64808_mouse-monoclonal-lrp-1-antibody-a2mr-alpha-2-31020241682149.jpg "Immunocytochemistry/ Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Increased sorting endosome size & decreased EHD1 recruitment upon Rabenosyn-5 knock-down.A-L, Representative micrographs & insets depicting EEA1-labeled endosomes & EHD1-GFP in mock-treated & Rabenosyn-5 knock-down cells. CRISPR/Cas9 gene-edited NIH3T3 EHD1-GFP cells were either mock-treated with transfection reagent (A-F) or treated with Rabenosyn-5 siRNA (G-L) for 72 h. Cells were then incubated with anti-LRP1 antibody (30 min on ice, 30 min at 37°C), fixed & immunostained using anti-EEA1, & imaged by confocal microscopy. M, Immunoblot showing reduced Rabenosyn-5 expression in EHD1-GFP NIH3T3 cells. N, Graph depicting mean endosome size of mock-treated & Rabenosyn-5 knock-down cells. O, Graph depicting EHD1 recruitment to endosomes in mock-treated & Rabenosyn-5 knock-down cells. Error bars denote standard deviation & p-values were determined by independent two-tailed t-test, with significance derived from consensus p-values from the 3 experiments. Micrographs are representative orthogonal projections from three independent experiments, with 10 sets of z-stacks collected for each treatment per experiment. Bar, 10 μm. **p < 0.00001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32966336), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")