Home » Lipoprotein Lipase/LPL » Lipoprotein Lipase/LPL Antibodies » Lipoprotein Lipase/LPL Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

Lipoprotein Lipase/LPL Antibody [Unconjugated]

 (11 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Lipoprotein Lipase/LPL at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody (Catalog ...read more
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and NMuMG mouse mammary gland epithelial cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Lipoprotein Lipase/LPL Antigen ...read more
Lipoprotein Lipase/LPL was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Goat Anti-Human Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7197) at 10 µg/mL for ...read more
Lipoprotein Lipase/LPL was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human/Mouse Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7197) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF7197-SP
100% Guarantee on every product

Lipoprotein Lipase/LPL Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Lipoprotein Lipase/LPL
Accession # P06858
Specificity
Detects human and mouse Lipoprotein Lipase/LPL in Western blots. Detects human Lipoprotein Lipase/LPL in direct ELISAs and less than 1% cross-reactivity with recombinant human (rh) LIPG, rhLIPI, and rhPNLIPRP1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LPL
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF7197 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipoprotein Lipase/LPL Antibody [Unconjugated]

  • EC 3.1.1
  • HDLCQ11
  • LIPD
  • LIPDEC 3.1.1.34
  • Lipoprotein Lipase
  • LPL

Background

LPL (LipoProtein Lipase; also LIPD) is a 53-56 kDa glycoprotein member of the Lipase family, AB Hydrolase superfamily of molecules. It is produced by multiple cell types, including adipocytes, skelelal muscle cells and macrophages. Once secreted, the circulating enzyme ultimately becomes immobilized on the surface of endothelium by binding to cell surface heparan sulfate. Here, it hydrolyzes triglycerides embedded in chylomicrons and VLDLs by homodimerizing and interacting with apoC2. Mature human LPL is 448 amino acids (aa) in length. It contains an enzymatic region (aa 37-334) plus one protein-interaction PLAT domain (aa 341-465). Over aa 28-154, human LPL shares 91% aa identity with mouse LPL.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

LIPC Antibody -
BSA Free
NBP3-04922
Immunocytochemistry/ Immunofluorescence LIPC Antibody - BSA Free
Species: Hu
Applications: ICC/IF, WB
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(24 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
Thyroglobulin
Antibody (2H11 + 6E1)
NBP2-34294
Immunohistochemistry-Paraffin Thyroglobulin Antibody (2H11 + 6E1)
Immunohistochemistry Thyroglobulin Antibody (2H11 + 6E1)
Immunohistochemistry-Paraffin Thyroglobulin Antibody (2H11 + 6E1)
(3 Reviews)
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(803 Publications)
Species: Hu
Applications: BA
PPAR gamma/NR1C3
Antibody - BSA Free
NBP2-22106
Western Blot PPAR gamma/NR1C3 Antibody - BSA Free
Immunohistochemistry-Paraffin PPAR gamma/NR1C3 Antibody - BSA Free
Western Blot PPAR gamma/NR1C3 Antibody - BSA Free
(2 Reviews)
(9 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
CETP Antibody
(ATM192) - BSA Free
NB300-558
Immunocytochemistry/ Immunofluorescence CETP Antibody (ATM192) - BSA Free
Western Blot CETP Antibody (ATM192) - BSA Free
(1 Publication)
Species: Bv, Hu, Rb
Applications: ELISA, ICC/IF, IP, WB
Apolipoprotein
C-II/ApoC2 Antibody [ ...
AF4497
Western Blot Apolipoprotein C-II/ApoC2 Antibody [Unconjugated]
Simple Western Apolipoprotein C-II/ApoC2 Antibody [Unconjugated]
(1 Publication)
Species: Hu
Applications: Simple Western, WB
HES-1 Antibody
(OTI4H1)
NBP1-47791
Western Blot HES-1 Antibody (OTI4H1)
Immunocytochemistry/ Immunofluorescence HES-1 Antibody (OTI4H1)
Immunocytochemistry/ Immunofluorescence HES-1 Antibody (OTI4H1)
(8 Publications)
Species: Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, WB
HMGCL Antibody
NBP1-32767
Immunocytochemistry/ Immunofluorescence HMGCL Antibody
Western Blot HMGCL Antibody
Immunohistochemistry-Paraffin HMGCL Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Hormone-sensitive
Lipase/HSL Antibody
NB110-37253
Western Blot Hormone-sensitive Lipase/HSL Antibody
(5 Publications)
Species: Hu, Mu, Rt
Applications: WB
Apolipoprotein
E/ApoE Antibody (WUE- ...
NB110-60531
Flow (Intracellular) Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free
Western Blot Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free
Flow Cytometry Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free
(2 Reviews)
(36 Publications)
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB
Apolipoprotein
A-I/ApoA1 Antibody - ...
NBP2-52979
Flow Cytometry Apolipoprotein A-I/ApoA1 Antibody - BSA Free
Western Blot Apolipoprotein A-I/ApoA1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Apolipoprotein A-I/ApoA1 Antibody - BSA Free
(1 Review)
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Apolipoprotein
CIII Antibody
NB600-610
Immunohistochemistry Apolipoprotein CIII Antibody
Immunohistochemistry-Paraffin Apolipoprotein CIII Antibody
(1 Review)
(2 Publications)
Species: Bv, Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
Fatty Acid
Synthase/FASN Antibod
NB400-114
Immunocytochemistry/ Immunofluorescence Fatty Acid Synthase/FASN Antibody
Western Blot Fatty Acid Synthase/FASN Antibody
Immunohistochemistry-Paraffin Fatty Acid Synthase/FASN Antibody
(2 Reviews)
(24 Publications)
Species: Ch, Fe, Ha, Hu, Mu, Pl, Po, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, IP, KD, WB
Leptin/OB [HRP]
DLP00
N/A Leptin/OB [HRP]
N/A Leptin/OB [HRP]
(150 Publications)
Species: Hu
Applications: ELISA
LDLR Antibody
[Unconjugated]
AF2255
Immunohistochemistry LDLR Antibody [Unconjugated]
Western Blot LDLR Antibody [Unconjugated]
Western Blot LDLR Antibody [Unconjugated]
(45 Publications)
Species: Mu
Applications: Block, CyTOF-ready, Flow, IHC, WB
PPAR alpha/NR1C1
Antibody (3B6/PPAR) - ...
NB300-537
Flow Cytometry PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free
Western Blot PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free
Flow Cytometry PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free
(1 Review)
(16 Publications)
Species: Bv, Hu, Mu, Rb, Rt
Applications: ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, KD, WB
Albumin Antibody
(188835) [Unconjugate ...
MAB1455
Simple Western Albumin Antibody (188835) [Unconjugated] - Serum
Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum
Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum
(3 Reviews)
(54 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
Endothelial Lipase
Antibody
NB400-111
Immunocytochemistry/ Immunofluorescence Endothelial Lipase Antibody
Western Blot Endothelial Lipase Antibody
Western Blot Endothelial Lipase Antibody
(6 Publications)
Species: Hu, Mu(-)
Applications: ICC/IF, IHC, IHC-Fr, WB
Lipoprotein
Lipase/LPL Antibody [ ...
AF7197
Lipoprotein Lipase/LPL Antibody [Unconjugated]
Species: Hu, Mu
Applications: WB, IHC, ICC/IF

Publications for Lipoprotein Lipase/LPL Antibody (AF7197)(11)

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 5 applications: ICC, IHC, Immunogold Electron Microscopy, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(1)
IHC
(1)
Immunogold Electron Microscopy
(1)
Immunoprecipitation
(1)
Western Blot
(4)
All Applications
Filter By Species
Mouse
(4)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF7197 Applications Species
Kraaijenhof, JM;Tromp, TR;Nurmohamed, NS;Reeskamp, LF;Langenkamp, M;Levels, JHM;Boekholdt, SM;Wareham, NJ;Hoekstra, M;Stroes, ESG;Hovingh, GK;Grefhorst, A; ANGPTL3 (Angiopoietin-Like 3) Preferentially Resides on High-Density Lipoprotein in the Human Circulation, Affecting Its Activity Journal of the American Heart Association 2023-10-27 [PMID: 37889183] (Western Blot, Mouse) Western Blot Mouse
Wu, SA;Shen, C;Wei, X;Zhang, X;Wang, S;Chen, X;Torres, M;Lu, Y;Lin, LL;Wang, HH;Hunter, AH;Fang, D;Sun, S;Ivanova, MI;Lin, Y;Qi, L; The mechanisms to dispose of misfolded proteins in the endoplasmic reticulum of adipocytes Nature communications 2023-05-30 [PMID: 37253728] (Immunoprecipitation, Western Blot, Immunogold Electron Microscopy, IHC, Transgenic Mouse, Mouse) Immunoprecipitation, Western Blot, Immunogold Electron Microscopy, IHC Transgenic Mouse, Mouse
Franziska Schmalz, Janett Fischer, Hamish Innes, Stephan Buch, Christine Möller, Madlen Matz-Soja et al. High producer variant of lipoprotein lipase may protect from hepatocellular carcinoma in alcohol-associated cirrhosis JHEP Reports 2023-04-01 [PMID: 36879887]
Nathalia Araujo, James Sledziona, Sunil K. Noothi, Ravshan Burikhanov, Nikhil Hebbar, Saptadwipa Ganguly et al. Tumor Suppressor Par-4 Regulates Complement Factor C3 and Obesity Frontiers in Oncology 2022-03-29 [PMID: 35425699]
Verena van der Heide, Sonia Jangra, Phillip Cohen, Raveen Rathnasinghe, Sadaf Aslam, Teresa Aydillo et al. Limited extent and consequences of pancreatic SARS-CoV-2 infection Cell Reports 2022-03-01 [PMID: 35247306]
B Roberts, C Yang, S Neher Characterization of lipoprotein lipase storage vesicles in 3T3-L1 adipocytes Journal of Cell Science, 2021-01-01;0(0):. 2021-01-01 [PMID: 34279659] (ICC, Western Blot, Mouse) ICC, Western Blot Mouse
Fujimoto BA, Young M, Nakamura N et al. Disrupted glucose homeostasis and skeletal muscle-specific glucose uptake in an exocyst knockout mouse model The Journal of biological chemistry 2/26/2021 [PMID: 33647317]
Federico Oldoni, Haili Cheng, Serena Banfi, Viktoria Gusarova, Jonathan C. Cohen, Helen H. Hobbs ANGPTL8 has both endocrine and autocrine effects on substrate utilization JCI Insight 2020-09-03 [PMID: 32730227]
Jonathan L. Warren, Sule Bulur, Fernando Ovalle, Samuel T. Windham, Barbara A. Gower, Gordon Fisher Effects of acute hyperinsulinemia on skeletal muscle mitochondrial function, reactive oxygen species production, and metabolism in premenopausal women Metab. Clin. Exp. 2017-12-01 [PMID: 29132536]
EWY Tung, S Ahmed, V Peshdary, E Atlas Firemaster� 550 and its components isopropylated triphenyl phosphate and triphenyl phosphate enhance adipogenesis and transcriptional activity of peroxisome proliferator activated receptor (Ppar?) on the adipocyte protein 2 (aP2) promoter PLoS ONE, 2017-04-24;12(4):e0175855. 2017-04-24 [PMID: 28437481] (Western Blot, Mouse) Western Blot Mouse
Show All 11 Publications.

Reviews for Lipoprotein Lipase/LPL Antibody (AF7197) (0)

There are no reviews for Lipoprotein Lipase/LPL Antibody (AF7197). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Lipoprotein Lipase/LPL Antibody (AF7197) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Lipoprotein Lipase/LPL Products

Blogs on Lipoprotein Lipase/LPL

There are no specific blogs for Lipoprotein Lipase/LPL, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog #AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog #F0107).
Goat IgG Isotype Control [Unconjugated]
AB-108-C
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Lipoprotein Lipase/LPL Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol LPL
Uniprot