Home » Apolipoprotein C-II/ApoC2 » ApoC2 Antibodies » Apolipoprotein C-II/ApoC2 Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

Apolipoprotein C-II/ApoC2 Antibody [Unconjugated]

 (1 Publication)
 
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of human small intestine tissue and plasma. PVDF membrane was probed with 0.5 µg/mL of Human Apolipoprotein C-II/ApoC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4497) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for precursor Apolipoprotein C-II/ApoC2 at approximately 12 kDa and processed ApoC2 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of human small intestine tissue and plasma. PVDF membrane was probed with 0.5 µg/mL of Human Apolipoprotein C-II/ApoC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4497) ...read more
Simple Western lane view shows lysates of human plasma, loaded at 0.2 mg/mL. A specific band was detected for Apolipoprotein C‑II/ApoC2 at approximately 9 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF4497
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4497-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Apolipoprotein C-II/ApoC2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human ApoC2
Thr23-Glu101
Accession # P02655
Specificity
Detects human ApoC2 in Western blots. In Western blots, this antibody detects both the precursor and processed forms of ApoC2.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
APOC2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publication using
AF4497 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Apolipoprotein C-II/ApoC2 Antibody [Unconjugated]

  • APC2
  • APOC2
  • Apo-CII
  • apoC-II
  • Apolipoprotein C2
  • Apolipoprotein CII
  • Apolipoprotein C-II
  • MGC75082

Background

Apolipoprotein C2 (ApoC2/II) is a 8‑9 kDa, secreted member of the Apolipoprotein C2 family of proteins. It is produced by hepatocytes and represents a major component of VLDL particles. It activates lipoprotein lipase and may self-associate to form amyloid-type fibrils. The human ApoC2 precursor is 101 amino acids (aa) in length. It contains a 22 amino acid (aa) signal sequence, followed by a 79 aa ProApoC2 that contains a lipid-binding region (aa 43‑51) and an enzyme interaction site (aa 55‑78). ProApoC2 represents >90% of circulating ApoC2. In human, limited proteolytic processing occurs with removal of the six aa prosegment (aa 23‑28). This does not affect activity. Human ProApoC2 is 59% and 62% aa identical to mouse and rat ProApoC2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Lipoprotein
Lipase/LPL Antibody [ ...
AF7197
Immunohistochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]
Western Blot Lipoprotein Lipase/LPL Antibody [Unconjugated]
Immunocytochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]
(12 Publications)
Species: Hu, Mu
Applications: ICC, IHC, WB
Apolipoprotein
CIII Antibody
NB600-610
Immunohistochemistry Apolipoprotein CIII Antibody
Immunohistochemistry-Paraffin Apolipoprotein CIII Antibody
(1 Review)
(2 Publications)
Species: Bv, Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
Apolipoprotein
A-I/ApoA1 Antibody - ...
NBP2-52979
Flow Cytometry Apolipoprotein A-I/ApoA1 Antibody - BSA Free
Western Blot Apolipoprotein A-I/ApoA1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Apolipoprotein A-I/ApoA1 Antibody - BSA Free
(1 Review)
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Plastin L Antibody
NBP2-19860
Immunocytochemistry/ Immunofluorescence Plastin L Antibody
Western Blot Plastin L Antibody
Immunocytochemistry/ Immunofluorescence Plastin L Antibody
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Apolipoprotein
A-II/ApoA2 Antibody ( ...
NBP3-16168
Immunohistochemistry Apolipoprotein A-II/ApoA2 Antibody (5F7U1)
Western Blot Apolipoprotein A-II/ApoA2 Antibody (5F7U1)
Immunohistochemistry Apolipoprotein A-II/ApoA2 Antibody (5F7U1)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, WB
Apolipoprotein C1
Antibody (2E2-1A3)
H00000341-M01
Western Blot Apolipoprotein C1 Antibody (2E2-1A3)
Western Blot Apolipoprotein C1 Antibody (2E2-1A3)
(3 Publications)
Species: Hu
Applications: ELISA, WB
Apolipoprotein
E/ApoE Antibody (WUE- ...
NB110-60531
Flow (Intracellular) Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free
Western Blot Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free
Flow Cytometry Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free
(2 Reviews)
(38 Publications)
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB
Thyroglobulin
Antibody (2H11 + 6E1)
NBP2-34294
Immunohistochemistry-Paraffin Thyroglobulin Antibody (2H11 + 6E1)
Immunohistochemistry Thyroglobulin Antibody (2H11 + 6E1)
Immunohistochemistry-Paraffin Thyroglobulin Antibody (2H11 + 6E1)
(3 Reviews)
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P
LIPC Antibody -
BSA Free
NBP3-04922
Immunocytochemistry/ Immunofluorescence LIPC Antibody - BSA Free
Species: Hu
Applications: ICC/IF, WB
Apolipoprotein
A-IV/ApoA4 Antibody ( ...
NBP2-52932
Western Blot Apolipoprotein A-IV/ApoA4 Antibody (CL0468)
Immunohistochemistry-Paraffin Apolipoprotein A-IV/ApoA4 Antibody (CL0468)
Immunohistochemistry-Paraffin Apolipoprotein A-IV/ApoA4 Antibody (CL0468)
(1 Review)
Species: Hu
Applications: IHC, IHC-P, WB
APC Antibody - BSA
Free
NB100-91662
Immunocytochemistry/ Immunofluorescence APC Antibody - BSA Free
Flow Cytometry APC Antibody - BSA Free
Immunohistochemistry-Paraffin APC Antibody - BSA Free
(2 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Coagulation Factor
XIV/Protein C Antibod ...
AF4885
Coagulation Factor XIV/Protein C Antibody
(2 Publications)
Species: Mu
Applications: IP, WB
Apolipoprotein
B/ApoB Antibody (3697 ...
MAB4124
Immunohistochemistry Apolipoprotein B/ApoB Antibody (369717) [Unconjugated]
Western Blot Apolipoprotein B/ApoB Antibody (369717) [Unconjugated]
Simple Western Apolipoprotein B/ApoB Antibody (369717) [Unconjugated]
(7 Publications)
Species: Hu
Applications: IHC, Simple Western, WB
Albumin Antibody
(188835) [Unconjugate ...
MAB1455
Simple Western Albumin Antibody (188835) [Unconjugated] - Serum
Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum
Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum
(3 Reviews)
(54 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
LDLR Antibody
[Unconjugated]
AF2255
Immunohistochemistry LDLR Antibody [Unconjugated]
Western Blot LDLR Antibody [Unconjugated]
Western Blot LDLR Antibody [Unconjugated]
(46 Publications)
Species: Mu
Applications: Block, CyTOF-ready, Flow, IHC, WB
Apolipoprotein A5
Antibody (1G5G9)
NB400-139
Western Blot Apolipoprotein A5 Antibody (1G5G9)
Western Blot Apolipoprotein A5 Antibody (1G5G9)
Flow Cytometry Apolipoprotein A5 Antibody (1G5G9)
(3 Publications)
Species: Ha, Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Complement C3
Antibody (11H9) - BSA ...
NB200-540
Flow Cytometry Complement C3 Antibody (11H9) - BSA Free
Immunocytochemistry/ Immunofluorescence Complement C3 Antibody (11H9) - BSA Free
Immunohistochemistry-Paraffin Complement C3 Antibody (11H9) - BSA Free
(2 Reviews)
(31 Publications)
Species: Ec, Mu
Applications: CyTOF-ready, Flow-IC, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Apolipoprotein
C-II/ApoC2 Antibody [ ...
AF4497
Apolipoprotein C-II/ApoC2 Antibody [Unconjugated]
Species: Hu
Applications: WB, Simple Western

Publications for Apolipoprotein C-II/ApoC2 Antibody (AF4497)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for Apolipoprotein C-II/ApoC2 Antibody (AF4497) (0)

There are no reviews for Apolipoprotein C-II/ApoC2 Antibody (AF4497). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Apolipoprotein C-II/ApoC2 Antibody (AF4497) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Apolipoprotein C-II/ApoC2 Products

Blogs on Apolipoprotein C-II/ApoC2

There are no specific blogs for Apolipoprotein C-II/ApoC2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Apolipoprotein C-II/ApoC2 Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol APOC2
Uniprot