Hormone-sensitive Lipase/HSL Antibody

HSL (hormone-sensitive lipase) is a key enzyme in lipid metabolism regulation in several tissues and this intracellular neutral lipase hydrolyzes triacylglycerols (TG), diacylglycerols (DG), and monoacylglycerols, as well as cholesterol and retinyl esters. While ATGL selectively performs the first step in TG hydrolysis, HSL is the most active enzyme against DG, which gets hydrolyzed faster than TG. HSL has been shown to express in several non-adipose tissues, including ovary, adrenal gland, heart, skeletal and smooth muscle, placenta, and pancreatic beta-cells, as well as testes. Under basal conditions, HSL is exclusively found in the cytosol of adipocytes, whereas upon phosphorylation mediated activation via PKA, HSL translocates from cytosol to lipid droplet surfaces for participation in lipolysis. HSL activity is regulated primarily by serine phosphorylation, which is stimulatory at Ser-563/Ser-659 via PKA and inhibitory at Ser-565 via AMPK. Mice lacking HSL have decreased nonesterified fatty acid (NEFA) and increased cholesterol concentrations in plasma and are resistant to diet-induced obesity. HSL has emerged as a potential target for lipid disorders and obesity.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 1 application: WB.

Showing Publications 1 - 5 of 5.

Publications using NB110-37253	Applications	Species
Chen L, You Q, Liu M et al. Remodeling of dermal adipose tissue alleviates cutaneous toxicity induced by anti-EGFR therapy eLife 2022-03-24 [PMID: 35324426]
Zhao F, Ke J, Pan W et al. Synergistic effects of ISL1 and KDM6B on non-alcoholic fatty liver disease through the regulation of SNAI1 Molecular medicine (Cambridge, Mass.) 2022-01-31 [PMID: 35100965] (WB, Mouse)	WB	Mouse
Smagris E, BasuRay S, Li J et al. Pnpla3I148M knockin mice accumulate PNPLA3 on lipid droplets and develop hepatic steatosis. Hepatology. 2014-06-10 [PMID: 24917523] (WB, Mouse) Details: Rabbit anti-mouse HSL (1:1000; Novus Biologicals, Littleton, CO) used in Western blot analysis of hepatic lipid droplet proteins of Pnpla3148M/M and Pnpla347A/A KI mice(Fugure 6A)	WB	Mouse
Hagopian K, Tomilov AA, Tomilova N et al. Shc proteins influence the activities of enzymes involved in fatty acid oxidation and ketogenesis Metabolism 2012-06-07 [PMID: 22683097] (WB, Mouse)	WB	Mouse
Kraemer, FB et al. Detection of hormone-sensitive lipase in various tissues. J Lipid Res. 34(4):663-71. 1993-04-01 [PMID: 8496671] (WB, Rat)	WB	Rat

Array NB110-37253

• Hormone-sensitive Lipase/HSL Antibodies
• Hormone-sensitive Lipase/HSL ELISA Kits
• Hormone-sensitive Lipase/HSL Lysate
• Hormone-sensitive Lipase/HSL Proteins