LAMP-2/CD107b Antibody (H4B4) - BSA Free

LAMP-2 (Lysosome-associated membrane protein 2) is a single-pass type I membrane protein that belongs to a family of membrane glycoproteins (~40 KDa). LAMP-2 protein is encoded by nine exons, with the first 8 exons and a portion of exon 9 encoding the highly glycosylated protein domains within the lysosomal lumen. The transmembrane and cytosolic carboxy-terminal domains of LAMP-2 are encoded by the remaining sequence of exon 9 and conform the receptor for targeting proteins to the lysosome. Splicing of exon 9 in the LAMP-2 pre mRNA leads to various splice forms with distinct cytosolic domains. Three splice variants, LAMP-2A, -2B and -2C, have been identified which shuttle between the plasma membrane, endosomal compartment and lysosomes (1). Tissue specific expression has been described for each LAMP-2 splice variant, with LAMP-2A being more ubiquitously expressed (e.g., placenta, lung, liver, pancreas and prostate), LAMP-2B predominantly expressed in skeletal muscle and LAMP-2C in brain tissue (1). All LAMP-2 splice variants participate in lysosomal degradation processes. LAMP-2A is the only variant that serves as a receptor targeting proteins for lysosomal degradation in chaperone-mediated autophagy (2,3). LAMP-2B is essential for macroautophagy in cardiomyocytes, where it facilitates autophagosome-lysosome fusion. LAMP-2B mutations underscore the myopathy and severe hypertrophic cardiomyopathy in Danon disease which results from deficits in autophagy (1, 4). Vasculopathy of coronary and cerebral arteries is a rare phenotype in Danon patients that is also associated with deficient autophagy processing of proteins and cellular organelles (5). LAMP2C serves as a receptor for DNA and RNA, facilitating their lysosomal degradation through DNA-autophagy and RNA-autophagy, respectively (1).

References

1. Rowland, T. J., Sweet, M. E., Mestroni, L., & Taylor, M. R. G. (2016). Danon disease - dysregulation of autophagy in a multisystem disorder with cardiomyopathy. Journal of Cell Science. https://doi.org/10.1242/jcs.184770

2. Alfaro, I. E., Albornoz, A., Molina, A., Moreno, J., Cordero, K., Criollo, A., & Budini, M. (2019). Chaperone mediated autophagy in the crosstalk of neurodegenerative diseases and metabolic disorders. Frontiers in Endocrinology. https://doi.org/10.3389/fendo.2018.00778

3. Schneider, J. L., & Cuervo, A. M. (2014). Autophagy and human disease: Emerging themes. Current Opinion in Genetics and Development. https://doi.org/10.1016/j.gde.2014.04.003

4. Chi, C., Leonard, A., Knight, W. E., Beussman, K. M., Zhao, Y., Cao, Y., Song, K. (2019). LAMP-2B regulates human cardiomyocyte function by mediating autophagosome lysosome fusion. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.1808618116

5. Nguyen, H. T., Noguchi, S., Sugie, K., Matsuo, Y., Nguyen, C. T. H., Koito, H., Tsukaguchi, H. (2018). Small-Vessel Vasculopathy Due to Aberrant Autophagy in LAMP-2 Deficiency. Scientific Reports. https://doi.org/10.1038/s41598-018-21602-8

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 8 applications: EM, IB, ICC/IF, IF, IF/IHC, IHC-Fr, IHC-P, WB.

Showing Publications 1 - 10 of 31. Show All 31 Publications. Collapse Publications.

Publications using NBP2-22217	Applications	Species
Fang R, Ming T, Ng JPL et al. Ciliatoside A, isolated from Peristrophe japonica, inhibits HBsAg expression and cccDNA transcription by inducing autophagy Antiviral research 2022-12-07 [PMID: 36496141]
Lee H, Nguyen Hoang AT, Lee SJ. Ginsenoside protopanaxadiol protects adult retinal pigment epithelial-19 cells from chloroquine by modulating autophagy and apoptosis PLOS ONE 2022-12-01 [PMID: 36454967]
Pillai S, Mahmud I, Mahar R et al. Lipogenesis mediated by OGR1 regulates metabolic adaptation to acid stress in cancer cells via autophagy Cell reports 2022-05-10 [PMID: 35545051] (ICC/IF, Human)	ICC/IF	Human
Fathi A, Mathivanan S, Kong L et al. Chemically induced senescence in human stem cell-derived neurons promotes phenotypic presentation of neurodegeneration Aging Cell 2022-01-01 [PMID: 34953016]
Nanou A, Bourbouli M, Vetrano S et al. Endothelial Tpl2 regulates vascular barrier function via JNK-mediated degradation of claudin-5 promoting neuroinflammation or tumor metastasis Cell reports 2021-05-25 [PMID: 34038728] (IHC-P, Mouse)	IHC-P	Mouse
Hirata H, Xu X, Nishioka K, et al. PMEPA1 and NEDD4 control the proton production of osteoclasts by regulating vesicular trafficking FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-02-01 [PMID: 33484199]
Yuniati L, Lauriola A, Gerritsen M et al. Ubiquitylation of the ER-Shaping Protein Lunapark via the CRL3KLHL12 Ubiquitin Ligase Complex Cell Rep 2020-05-19 [PMID: 32433973]
Price C, Jones S, Mihelcic M et al. Paradoxical Pro-inflammatory Responses by Human Macrophages to an Amoebae Host-Adapted Legionella Effector Cell Host Microbe 2020-03-30 [PMID: 32220647]
Ahmed M, Baumgartner R, Aldi S et al. Human serum albumin-based probes for molecular targeting of macrophage scavenger receptors Int J Nanomedicine 2019-05-21 [PMID: 31190821] (IF, Human)	IF	Human
Chang-Graham AL, Danhof HA, Engevik MA et al. Iron supply via NCOA4-mediated ferritin degradation maintains mitochondrial functions Mol. Cell. Biol. 2019-05-06 [PMID: 31061094]
Wong M, Ganapathy AS, Suchanec E et al. Intestinal Epithelial Tight Junction Barrier regulation by Autophagy related protein ATG6/beclin 1 Am. J. Physiol., Cell Physiol. 2019-03-20 [PMID: 30892937] (ICC/IF, Human)	ICC/IF	Human
Fang EF, Hou Y, Palikaras K, et al Mitophagy inhibits amyloid-beta and tau pathology and reverses cognitive deficits in models of Alzheimer's disease. Nat Neurosci. 2019 Feb 11 [PMID: 30742114] (EM, WB)	EM, WB
Fang EF, Hou Y, Palikaras K, et al Mitophagy inhibits amyloid-b and tau pathology and reverses cognitive deficits in models of Alzheimer's disease Nat. Neurosci. 2019 Feb 11 [PMID: 30742114] (EM, WB)	EM, WB
Masso-Welch P, Girald Berlingeri S, King-Lyons ND et al. LT-IIc, A Bacterial Type II Heat-Labile Enterotoxin, Induces Specific Lethality in Triple Negative Breast Cancer Cells by Modulation of Autophagy and Induction of Apoptosis and Necroptosis. Int J Mol Sci 2018-12-26 [PMID: 30587795] (ICC/IF, Human)	ICC/IF	Human
Jones JR, kong L, Hanna MG et al. Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes. Cell Rep. 2018-10-23 [PMID: 30355500] (ICC/IF, Mouse)	ICC/IF	Mouse
Furuya N, Kakuta S, Sumiyoshi K et al. NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy. EMBO Rep 2018-10-11 [PMID: 30309841] (Human)		Human
Fauzia E, Barbhuyan Tk, Shrivastava Ak et al. Chick Embryo: A Preclinical Model for Understanding Ischemia-Reperfusion Mechanism. Front Pharmacol. 2018-09-21 [PMID: 30298003] (WB, Chicken)	WB	Chicken
Swystun LL, Lai JD, Notley C et al. The endothelial cell receptor stabilin-2 regulates VWF-FVIII complex half-life and immunogenicity J. Clin. Invest. 2018-08-31 [PMID: 30124466] (ICC/IF, Human)	ICC/IF	Human
Pinazza Marica, Ghisi Margherita, Minuzzo Sonia et al. Histone deacetylase 6 controls Notch3 trafficking and degradation in T-cell acute lymphoblastic leukemia cells. Oncogene 2018-04-12 [PMID: 29643474] (IB, IF, Mouse, Human)	IB, IF	Mouse, Human
Nguyen HT, Noguchi S, Sugie K, Matsuo Y. Small-Vessel Vasculopathy Due to Aberrant Autophagy in LAMP-2 Deficiency. Sci Rep. 2018-02-20 [PMID: 29463847] (ICC/IF, Human)	ICC/IF	Human
Lee WH, Higuchi H, Ikeda S et al. Mouse Tmem135 mutation reveals a mechanism involving mitochondrial dynamics that leads to age-dependent retinal pathologies. Elife. 2016-11-15 [PMID: 27863209] (WB, Mouse)	WB	Mouse
Nighot PK, Hu CA, Ma TY. Autophagy enhances intestinal epithelial tight junction barrier function by targeting claudin-2 protein degradation. J Biol Chem 2015-03-13 [PMID: 25616664] (Human)		Human
Choi Yp, Head Mw, Ironside Jw, Priola Sa. Uptake and Degradation of Protease-Sensitive and -Resistant Forms of Abnormal Human Prion Protein Aggregates by Human Astrocytes. Am J Pathol 2014-09-29 [PMID: 25280631] (ICC/IF, Human) Details: Using the Alexa Fluor (R) 488 conjugated version of NBP2-22217, catalog number NBP2-22217AF488.	ICC/IF	Human
Bhandari D, Trejo J, Benovic JL, Marchese A. Arrestin-2 interacts with the ubiquitin-protein isopeptide ligase atrophin-interacting protein 4 and mediates endosomal sorting of the chemokine receptor CXCR4. J Biol Chem. 2007-12-21 [PMID: 17947233] (ICC/IF, Human)	ICC/IF	Human
Iwata A, Riley BE, Johnston JA, Kopito RR. HDAC6 and microtubules are required for autophagic degradation of aggregated huntingtin. J Biol Chem. 2005-12-02 [PMID: 16192271] (ICC/IF, Human)	ICC/IF	Human
Zhang Y, Higashide W, Dai S et al. Recognition and ubiquitination of Salmonella type III effector SopA by a ubiquitin E3 ligase, HsRMA1. J Biol Chem. 2005-11-18 [PMID: 16176924] (WB, Human)	WB	Human
Kunzli BM, Berberat PO, Zhu ZW et al. Influences of the lysosomal associated membrane proteins (Lamp-1, Lamp-2) and Mac-2 binding protein (Mac-2-BP) on the prognosis of pancreatic carcinoma. Cancer. 2002-01-01 [PMID: 11815981] (IF/IHC, Human)	IF/IHC	Human
Nishino I, Fu J, Tanji et al. Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease). Nature. 2000-08-24 [PMID: 10972294] (WB, IF/IHC, Human)	WB, IF/IHC	Human
Warren RA, Green FA, Stenberg PE, Enns CA. Distinct saturable pathways for the endocytosis of different tyrosine motifs. J Biol Chem. 1998-07-03 [PMID: 9642270]
Garcia-del Portillo F, Stein MA, Finlay BB. Release of lipopolysaccharide from intracellular compartments containing Salmonella typhimurium to vesicles of the host epithelial cell. Infect Immun. 1997-01-01 [PMID: 8975888] (ICC/IF, Human)	ICC/IF	Human
Chen C, Hashem S, Sharma J et al. Hematopoietic Stem Cell Gene Therapy Alleviates Disease Phenotypes in a Murine Model of Danon Disease Research Square 2022-08-19 (IHC-Fr, Mouse)	IHC-Fr	Mouse
Show All 31 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Verified Customer	ELISA	Human	06/05/2018	View Summary Application ELISA Sample Tested SVG-A cells Species Human Lot A-6
	5 reviewed by: Verified Customer	WB	Human	06/03/2016	View Summary Application Western Blot Sample Tested human macrophage whole cell lysate Species Human Lot A-3

Array NBP2-22217

• LAMP-2/CD107b Antibodies
• LAMP-2/CD107b ELISA Kits
• LAMP-2/CD107b Lysate
• LAMP-2/CD107b Proteins
• LAMP-2/CD107b Proteins and Enzymes