| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36568SS | |||
| Azide and BSA Free | NBP2-80711 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | IC8047G | |||
| Biotin | BAF8047 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC8047G | |||
| Carrier Free | AF8047-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8047-SP | |||
![Western Blot: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of partial recombinant human EEA1 protein (14kDa) with EEA1 antibody clone 6D4.1D4 at 0.5 ug mL.](http://images.novusbio.com/fullsize/EEA1-Antibody-6D4-1D4-Western-Blot-NBP2-36568-img0006.jpg "Western Blot: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of partial recombinant human EEA1 protein (14kDa) with EEA1 antibody clone 6D4.1D4 at 0.5 ug mL.")
![Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of FFPE tissue section of human testes using mouse monoclonal EEA1 antibody (clone 6D4.1D4) at 10 ug/ml concentration. The interstitial cells depicted a punctate to granular cytoplasmic staining pattern for EEA1 protein.](http://images.novusbio.com/fullsize/EEA1-Antibody-6D4-1D4-Immunohistochemistry-Paraffin-NBP2-36568-img0004.jpg)
![Flow (Intracellular): EEA1 Antibody (6D4.1D4) [NBP2-36568] - Intracellular staining of human EEA1 in Flow cytometry using 2.5 ug of antibody per 1 million cells. Isotype control was mouse IgG1.](http://images.novusbio.com/fullsize/EEA1-Antibody-6D4-1D4-Flow-Intracellular-NBP2-36568-img0002.jpg)
![Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of FFPE tissue section of mouse testes using mouse monoclonal EEA1 antibody (clone 6D4.1D4) at 10 ug/ml concentration. The interstitial cells depicted a punctate to granular cytoplasmic staining pattern for EEA1 protein with no signal in other cell types.](http://images.novusbio.com/fullsize/EEA1-Antibody-6D4-1D4-Immunohistochemistry-Paraffin-NBP2-36568-img0005.jpg)
![Western Blot: EEA1 Antibody [NBP1-30914] - 30 ug each lane of HEK-293 cell extracts. Image from verified customer review.](http://images.novusbio.com/fullsize/EEA1-Antibody-Western-Blot-NBP1-30914-img0023.jpg "Western Blot: EEA1 Antibody [NBP1-30914] - 30 ug each lane of HEK-293 cell extracts. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: EEA1 Antibody [NBP1-30914] - DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: EEA1 protein stained by EEA1 antibody at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody (NBP2-43561) at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/EEA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30914-img0022.jpg)
![Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914] - Paraffin-embedded colon carcinoma. Antibody at 1:500.](http://images.novusbio.com/fullsize/EEA1-Antibody-Immunohistochemistry-Paraffin-NBP1-30914-img0016.jpg)
![Western Blot: EEA1 Antibody [NBP1-30914] - Mouse brain. 5% SDS PAGE. Antibody at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EEA1-Antibody-Western-Blot-NBP1-30914-img0017.jpg)
![Western Blot: EEA1 Antibody [NBP1-30914] - A. 30 ug PC-12 whole cell lysate/extract, B. 30 ug Rat 2 whole cell lysate/extract. 5% SDS-PAGE. Antibody at 1:1000.](http://images.novusbio.com/fullsize/EEA1-Antibody-Western-Blot-NBP1-30914-img0018.jpg)
![Western Blot: EEA1 Antibody [NBP1-30914] - 30 ug whole cell lysate/extracts B. Antibody at 1:5000.](http://images.novusbio.com/fullsize/EEA1-Antibody-Western-Blot-NBP1-30914-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: EEA1 Antibody [NBP1-30914] - Methanol-fixed HeLa cells, using EEA1 (NBP1-30914) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled red.](http://images.novusbio.com/fullsize/EEA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30914-img0014.jpg)
![Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914] - Mouse testis. EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/EEA1-Antibody-Immunohistochemistry-Paraffin-NBP1-30914-img0013.jpg)
![Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914] - Mouse testis. EEA1 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/EEA1-Antibody-Immunohistochemistry-Paraffin-NBP1-30914-img0012.jpg)
![Western Blot: EEA1 Antibody [NBP1-05962] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. EEA1 was also immunoprecipitated by rabbit anti-EEA1 antibodies NBP1-05961 and NBP1-05963.](http://images.novusbio.com/fullsize/EEA1-Antibody-Western-Blot-NBP1-05962-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: EEA1 Antibody [NBP1-05962] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. EEA1 was also immunoprecipitated by rabbit anti-EEA1 antibodies NBP1-05961 and NBP1-05963.")
![Western Blot: EEA1 Antibody (GT10811) [NBP3-13599] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EEA1 antibody [GT10811] (NBP3-13599) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EEA1-Antibody-GT10811-Western-Blot-NBP3-13599-img0001.jpg "Western Blot: EEA1 Antibody (GT10811) [NBP3-13599] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EEA1 antibody [GT10811] (NBP3-13599) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: EEA1 Antibody (GT10811) [NBP3-13599] - EEA1 antibody [GT10811] detects EEA1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EEA1 stained by EEA1 antibody [GT10811] (NBP3-13599) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EEA1-Antibody-GT10811-Immunocytochemistry-Immunofluorescence-NBP3-13599-img0004.jpg)
![Immunohistochemistry-Paraffin: EEA1 Antibody (GT10811) [NBP3-13599] - EEA1 antibody [GT10811] detects EEA1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. EEA1 stained by EEA1 antibody [GT10811] (NBP3-13599) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EEA1-Antibody-GT10811-Immunohistochemistry-Paraffin-NBP3-13599-img0003.jpg)
![Immunohistochemistry-Paraffin: EEA1 Antibody (GT10811) [NBP3-13599] - EEA1 antibody [GT10811] detects EEA1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. EEA1 stained by EEA1 antibody [GT10811] (NBP3-13599) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EEA1-Antibody-GT10811-Immunohistochemistry-Paraffin-NBP3-13599-img0002.jpg)
![Western Blot: EEA1 Antibody (2G2) [H00008411-M03] - Analysis of EEA1 expression in A-431 (Cat # L015V1).](http://images.novusbio.com/fullsize/EEA1-Antibody-2G2-Western-Blot-H00008411-M03-img0004.jpg "Western Blot: EEA1 Antibody (2G2) [H00008411-M03] - Analysis of EEA1 expression in A-431 (Cat # L015V1).")
![Immunocytochemistry/Immunofluorescence: EEA1 Antibody (2G2) [H00008411-M03] - Analysis of monoclonal antibody to EEA1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/EEA1-Antibody-2G2-Immunocytochemistry-Immunofluorescence-H00008411-M03-img0003.jpg)
![ELISA: EEA1 Antibody (2G2) [H00008411-M03] - Detection limit for recombinant GST tagged EEA1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/EEA1-Antibody-2G2-ELISA-H00008411-M03-img0002.jpg)
![Western Blot: EEA1 Antibody (JE59-34) [NBP3-32292] - Western blot analysis of EEA1 on different lysates with Rabbit anti-EEA1 antibody (NBP3-32292) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: JAR cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C6 cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 162 kDa Observed band size: 162 kDa Exposure time: 12 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32292) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32292_rabbit-eea1-mab-je59-34-27820241631610.jpg "Western Blot: EEA1 Antibody (JE59-34) [NBP3-32292] - Western blot analysis of EEA1 on different lysates with Rabbit anti-EEA1 antibody (NBP3-32292) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: JAR cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C6 cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 162 kDa Observed band size: 162 kDa Exposure time: 12 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32292) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: EEA1 Antibody (JE59-34) [NBP3-32292] - Immunocytochemistry analysis of JAR cells labeling EEA1 with Rabbit anti-EEA1 antibody (NBP3-32292) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EEA1 antibody (NBP3-32292) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32292_rabbit-eea1-mab-je59-34-29202410234216.jpg)
![Flow Cytometry: EEA1 Antibody (JE59-34) [NBP3-32292] - Flow cytometric analysis of JAR cells labeling EEA1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32292, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32292_rabbit-eea1-mab-je59-34-2920241645322.jpg)
![Western Blot: EEA1 Antibody (M347) [NBP3-23301] - Western blot of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-EEA1 antibody at 1:500 (lane 1) or 1:1000 (lane 2).](http://images.novusbio.com/fullsize/nbp3-23301_mouse-eea1-mab-m347-19920231856958.jpg "Western Blot: EEA1 Antibody (M347) [NBP3-23301] - Western blot of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-EEA1 antibody at 1:500 (lane 1) or 1:1000 (lane 2).")
![Immunocytochemistry/Immunofluorescence: EEA1 Antibody (M347) [NBP3-23301] - Immunocytochemical labeling of EEA1 in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The cells were labeled with mouse monoclonal EEA1 (NBP3-23301). The antibody was detected using goat anti-mouse DyLight(R) 488.](http://images.novusbio.com/fullsize/nbp3-23301_mouse-eea1-mab-m347-19920231856971.jpg)
![Western Blot: EEA1 Antibody [NBP1-91859] - Analysis in human cell line SiHa.](http://images.novusbio.com/fullsize/EEA1-Antibody-Western-Blot-NBP1-91859-img0006.jpg "Western Blot: EEA1 Antibody [NBP1-91859] - Analysis in human cell line SiHa.")
![Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-91859] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/EEA1-Antibody-Immunohistochemistry-Paraffin-NBP1-91859-img0002.jpg)
![Western Blot: EEA1 Antibody (SR1246) [NBP3-21679] - Western blot analysis using NBP3-21679 in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21679_rabbit-eea1-mab-sr1246-57202315584818.jpg "Western Blot: EEA1 Antibody (SR1246) [NBP3-21679] - Western blot analysis using NBP3-21679 in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.")
![Western Blot: EEA1 Antibody (8S10A7) [NBP3-16537] - Western blot analysis of extracts of various cell lines, using EEA1 Rabbit mAb (NBP3-16537) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/EEA1-Antibody-8S10A7-Western-Blot-NBP3-16537-img0001.jpg "Western Blot: EEA1 Antibody (8S10A7) [NBP3-16537] - Western blot analysis of extracts of various cell lines, using EEA1 Rabbit mAb (NBP3-16537) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")