![Western Blot: Rabex5 Antibody [NBP1-49938] - Detection of Human and Mouse RABGEF1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RABGEF1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RABGEF1 was also immunoprecipitated by rabbit anti-RABGEF1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated RABGEF1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/Rabex5-Antibody-Western-Blot-NBP1-49938-img0001.jpg "Western Blot: Rabex5 Antibody [NBP1-49938] - Detection of Human and Mouse RABGEF1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RABGEF1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RABGEF1 was also immunoprecipitated by rabbit anti-RABGEF1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated RABGEF1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
| Reactivity | Hu, Mu, BvSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen for this product maps to a region between residue 175 and 225 of human RAB Guanine Nucleotide Exchange Factor (GEF) 1 using the numbering given in entry BAC87138.1 (GeneID 27342). |
| Predicted Species | Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RABGEF1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Rabex5 Antibody (NBP1-49938)Find related products by research area.
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| Gene Symbol | RABGEF1 |
![Western Blot: Rabex5 Antibody [NBP1-49938] - RABGEF1 is important for mitochondrial clearance. YFP-Parkin stably expressing WT and RABGEF1-mAID HCT116 cells pre-treated with IAA were treated with valinomycin for the indicated times. Total cell lysates were analyzed by immunoblotting. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/31326), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rabex5-Antibody-Western-Blot-NBP1-49938-img0005.jpg "Western Blot: Rabex5 Antibody [NBP1-49938] - RABGEF1 is important for mitochondrial clearance. YFP-Parkin stably expressing WT and RABGEF1-mAID HCT116 cells pre-treated with IAA were treated with valinomycin for the indicated times. Total cell lysates were analyzed by immunoblotting. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/31326), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Rabex5 Antibody [NBP1-49938] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- RABGEF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Rabex5-Antibody-Immunohistochemistry-Paraffin-NBP1-49938-img0004.jpg "Immunohistochemistry-Paraffin: Rabex5 Antibody [NBP1-49938] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- RABGEF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Western Blot: Rabex5 Antibody [NBP1-49938] - RABGEF1 recruitment to mitochondria during mitophagy.(A) The indicated cells were treated with DMSO or valinomycin for 3 hr followed by immunostaining. Bars, 10 μm. Graphs for quantification of RABGEF1 recruitment to mitochondria were shown below the images. None, partial & complete denote that GFP-mRABGEF1 signals were overlapped with no, some of, & all mitochondria, respectively. The error bars represent mean ±SE & over 100 cells were counted in each of three separate wells. (B) WT & TBC1D15/17 DKO HCT116 cells stably expressing mCherry-Parkin & GFP-mRABGEF1 were treated with DMSO or valinomycin for 3 hr. GFP-mRABGEF1 signals were enhanced by immunostaining with anti-GFP antibody. Bars, 10 μm. (C) Total cell lysates in (B) were analyzed by immunoblotting. * & # denote ubiquitinated forms & truncated forms, respectively.10.7554/eLife.31326.031Figure 8—figure supplement 1—source data 1.This excel file contains quantification of RABGEF1 (WT & Y26A/A58D mutant) recruitment to mitochondria in HCT116 (WT & TBC1D15/17 DKO) cells.This excel file contains quantification of RABGEF1 (WT & Y26A/A58D mutant) recruitment to mitochondria in HCT116 (WT & TBC1D15/17 DKO) cells. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/31326), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-49938_rabbit-polyclonal-rabex5-antibody-310202416182628.jpg "Western Blot: Rabex5 Antibody [NBP1-49938] - RABGEF1 recruitment to mitochondria during mitophagy.(A) The indicated cells were treated with DMSO or valinomycin for 3 hr followed by immunostaining. Bars, 10 μm. Graphs for quantification of RABGEF1 recruitment to mitochondria were shown below the images. None, partial & complete denote that GFP-mRABGEF1 signals were overlapped with no, some of, & all mitochondria, respectively. The error bars represent mean ±SE & over 100 cells were counted in each of three separate wells. (B) WT & TBC1D15/17 DKO HCT116 cells stably expressing mCherry-Parkin & GFP-mRABGEF1 were treated with DMSO or valinomycin for 3 hr. GFP-mRABGEF1 signals were enhanced by immunostaining with anti-GFP antibody. Bars, 10 μm. (C) Total cell lysates in (B) were analyzed by immunoblotting. * & # denote ubiquitinated forms & truncated forms, respectively.10.7554/eLife.31326.031Figure 8—figure supplement 1—source data 1.This excel file contains quantification of RABGEF1 (WT & Y26A/A58D mutant) recruitment to mitochondria in HCT116 (WT & TBC1D15/17 DKO) cells.This excel file contains quantification of RABGEF1 (WT & Y26A/A58D mutant) recruitment to mitochondria in HCT116 (WT & TBC1D15/17 DKO) cells. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/31326), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Rabex5 Antibody [NBP1-49938] - RABGEF1 is important for mitochondrial clearance.(A) WT & RABGEF1-mAID HCT116 cells were treated with or without IAA for 16 hr. Total cell lysates were analyzed by immunoblotting. (B) Quantification of Parkin recruitment to mitochondria in WT & RABGEF1-mAID HCT116 cells after 3 hr of valinomycin treatment. Partial & complete denote that YFP-Parkin signals were overlapped with some of & all mitochondria, respectively. (C) YFP-Parkin stably expressing WT & RABGEF1-mAID HCT116 cells pre-treated with IAA were treated with valinomycin for the indicated times. Total cell lysates were analyzed by immunoblotting. (D) WT & RABGEF1-mAID HCT116 cells stably expressing YFP-Parkin & mt-mKeima were treated with IAA for 16 hr followed by DMSO or OAQ for 6 hr & subjected to FACS analysis. Plots are representative of n = 3 experiments. (E) Quantification of mitophagy in (D). Error bars represent mean ±SE of three independent experiments. Statistical differences were determined by student’s t-test. *p<0.05.10.7554/eLife.31326.035Figure 9—source data 1.Quantification of YFP-Parkin recruitment to mitochondria in RABGEF1-mAID HCT116 & the corresponding WT cells during mitophagy.10.7554/eLife.31326.036Figure 9—source data 2.Quantification of mitophagy using mt-mKeima & FACS analysis.Quantification of YFP-Parkin recruitment to mitochondria in RABGEF1-mAID HCT116 & the corresponding WT cells during mitophagy.Quantification of mitophagy using mt-mKeima & FACS analysis. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/31326), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-49938_rabbit-polyclonal-rabex5-antibody-310202416182635.jpg "Western Blot: Rabex5 Antibody [NBP1-49938] - RABGEF1 is important for mitochondrial clearance.(A) WT & RABGEF1-mAID HCT116 cells were treated with or without IAA for 16 hr. Total cell lysates were analyzed by immunoblotting. (B) Quantification of Parkin recruitment to mitochondria in WT & RABGEF1-mAID HCT116 cells after 3 hr of valinomycin treatment. Partial & complete denote that YFP-Parkin signals were overlapped with some of & all mitochondria, respectively. (C) YFP-Parkin stably expressing WT & RABGEF1-mAID HCT116 cells pre-treated with IAA were treated with valinomycin for the indicated times. Total cell lysates were analyzed by immunoblotting. (D) WT & RABGEF1-mAID HCT116 cells stably expressing YFP-Parkin & mt-mKeima were treated with IAA for 16 hr followed by DMSO or OAQ for 6 hr & subjected to FACS analysis. Plots are representative of n = 3 experiments. (E) Quantification of mitophagy in (D). Error bars represent mean ±SE of three independent experiments. Statistical differences were determined by student’s t-test. *p<0.05.10.7554/eLife.31326.035Figure 9—source data 1.Quantification of YFP-Parkin recruitment to mitochondria in RABGEF1-mAID HCT116 & the corresponding WT cells during mitophagy.10.7554/eLife.31326.036Figure 9—source data 2.Quantification of mitophagy using mt-mKeima & FACS analysis.Quantification of YFP-Parkin recruitment to mitochondria in RABGEF1-mAID HCT116 & the corresponding WT cells during mitophagy.Quantification of mitophagy using mt-mKeima & FACS analysis. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/31326), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")