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RAPGEF3 Antibody - BSA Free

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Western Blot: RAPGEF3 Antibody [NBP2-81767] - Analysis of RAPGEF3 in rat skeletal muscle tissue lysate with RAPGEF3 antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: RAPGEF3 Antibody [NBP2-81767] - Immunofluorescence of RAPGEF3 in rat brain tissue with RAPGEF3 antibody at 20 ug/mL.
Immunohistochemistry: RAPGEF3 Antibody [NBP2-81767] - Immunohistochemistry of RAPGEF3 in rat brain tissue with RAPGEF3 antibody at 2.5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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RAPGEF3 Antibody - BSA Free Summary

Immunogen
RAPGEF3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human RAPGEF3. The immunogen is located within amino acids 60 - 110 of RAPGEF3. Amino Acid Squence: WTPLTNSEESLDFSESLE
Specificity
At least two isoforms of RAPGEF3 are known to exist; this antibody will detect both isoforms. RAPGEF3 antibody is predicted to not cross-react with EPAC2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAPGEF3
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 1 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for RAPGEF3 Antibody - BSA Free

  • 9330170P05Rik
  • bcm910
  • cAMP-GEFI
  • cAMP-regulated guanine nucleotide exchange factor I
  • CGEF1
  • EPAC 1
  • EPAC
  • EPAC1
  • EPACRAP guanine-nucleotide-exchange factor (GEF) 3
  • Exchange factor directly activated by cAMP 1
  • Exchange protein directly activated by cAMP 1
  • HSU79275
  • MGC21410
  • Rap guanine nucleotide exchange factor (GEF) 3
  • rap guanine nucleotide exchange factor 3
  • Rap1 guanine-nucleotide-exchange factor directly activated by cAMP
  • RAPGEF3

Background

RAPGEF3, also known as Rap guanine nucleotide exchange factor 3, is a 104 kDa 923 amino acid protein with two shorter isoforms produced by alternative splicing. The gene is necessary for rearranging actin at cell to cell junctions. RAPGEF3 is currently being studied for its involvement with leukemia, melanoma, pancreatitis, thyroiditis, carcinoma, prostatitis and neuronitis. RAPGEF3 is linked to the chemotaxis, cell adhesion, insulin secretion, and immune system pathways where it interacts with GNAI2, GNAI1, PDE3B, RAP1A and RAP1B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RAPGEF3