RAP1GAP Antibody

Images

 
Immunohistochemistry: RAP1GAP Antibody [NBP1-83002] - Staining of mouse hippocampus shows cytoplasmic positivity in a subset of interneurons in the CA1 area.
Immunohistochemistry-Paraffin: RAP1GAP Antibody [NBP1-83002] - Staining of human kidney shows strong positivity in distal tubules and Bowman's capsule.
Immunohistochemistry-Paraffin: RAP1GAP Antibody [NBP1-83002] - Staining of human hippocampus shows cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: RAP1GAP Antibody [NBP1-83002] - Staining of human cerebral cortex shows cytoplasmic immunoreactivity in neuronal cell bodies.
Immunohistochemistry-Paraffin: RAP1GAP Antibody [NBP1-83002] - Staining of human cerebellum shows strong cytoplasmic immunoreactivity in Purkinje cells and neurons in the molecular layer.
Immunohistochemistry: RAP1GAP Antibody [NBP1-83002] - Staining of mouse midbrain shows strong immunoreactivity in locus coeruleus neurons.
Immunohistochemistry: RAP1GAP Antibody [NBP1-83002] - Staining of mouse cingulate cortex shows strong cytoplasmic positivity in neuronal cell bodies and proximal dendrites.
Immunohistochemistry: RAP1GAP Antibody [NBP1-83002] - Staining of mouse hypothalamus shows positivity in neuronal cell bodies and processes in the lateral preoptic area.
Immunohistochemistry: RAP1GAP Antibody [NBP1-83002] - Staining of mouse globus pallidus shows strong cytoplasmic immunoreactivity in neurons.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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RAP1GAP Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: IGIENIQEVQEKRESPPAGQKTPDSGHVSQEPKSENSSTQSSPEMPTTKNRAETAAQRAEALKDFSRSSSSASSFASVVEETEGVDGEDTGLESVSSSGTP
Predicted Species
Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAP1GAP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RAP1GAP Protein (NBP1-83002PEP)
Publications
Read Publication using NBP1-83002.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RAP1GAP Antibody

  • GTPase activating protein 1
  • RAP1 GTPase activating protein

Background

Rap1GAP, an 85/95kDa protein, is a member of a family of GAP's specific for Rap1 (1). Though to be an important tumor suppressor, Rap1GAP has no sequence homology with other GAPs such as RasGAP, RanGAP and RhoGAP. Purified as a cytosolic and membrane-bound protein, both isoforms consist of two monomers each contain a dimerization and a catalytic domain (2-4). Mutations in the GAP domain of the tumor suppressor tuberin, can cause benign tumor phenotype tuberous sclerosis (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RAP1GAP Antibody (NBP1-83002)(1)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RAP1GAP