![Western Blot: Mouse Brain Whole Tissue Lysate (Adult Normal) [NB820-59657] - Western blot analysis of GAPDH (NB300-323) using whole normal mouse brain lysate [NB820-59657].](http://images.novusbio.com/fullsize/Mouse-Brain-Whole-Tissue-Lysate-Adult-Normal-Western-Blot-NB820-59657-img0005.jpg "Western Blot: Mouse Brain Whole Tissue Lysate (Adult Normal) [NB820-59657] - Western blot analysis of GAPDH (NB300-323) using whole normal mouse brain lysate [NB820-59657].")
| Reactivity | MuSpecies Glossary |
| Applications | WB, IP, PAGE |
| Concentration | 5 mg/ml |
| Description | We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use. |
| Specificity | Total Protein - Mouse Normal Tissue: Brain |
| Dilutions |
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| Application Notes | Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors |
| Concentration | 5 mg/ml |
| Type | Tissue |
| Life Stage | Adult |
| Tissue | Brain |
| Protein State | Native |
| Tissue Condition | Normal |
| Subcellular Fraction | Whole |
|
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![SDS-Page: Mouse Brain Whole Tissue Lysate (Adult Normal) [NB820-59657] - Ponceau S staining of whole normal mouse brain lysate [NB820-59657].](http://images.novusbio.com/fullsize/Mouse-Brain-Whole-Tissue-Lysate-Adult-Normal-SDS-Page-NB820-59657-img0004.jpg "SDS-Page: Mouse Brain Whole Tissue Lysate (Adult Normal) [NB820-59657] - Ponceau S staining of whole normal mouse brain lysate [NB820-59657].")
![SDS-Page: Mouse Brain Whole Tissue Lysate (Whole Normal) [NB820-59657] - Protein lysate is positively confirmed by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.](http://images.novusbio.com/fullsize/Mouse-Brain-Whole-Tissue-Lysate-Whole-Normal-SDS-Page-NB820-59657-img0006.jpg "SDS-Page: Mouse Brain Whole Tissue Lysate (Whole Normal) [NB820-59657] - Protein lysate is positively confirmed by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.")