Immunocytochemistry/ Immunofluorescence: Myelin Protein Zero Antibody [NB100-1607] - Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of ...read more
Immunohistochemistry: Myelin Protein Zero Antibody [NB100-1607] - In the image (a lower power tissue section through an adult sciatic nerve), Myelin Protein Zero (brown staining) can be seen in all of the myelinating ...read more
Chickens were immunized with two synthetic peptide/keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of Myelin Protein Zero, but are shared between the human (NP_000521, NCBI) and mouse (NP_032649, NCBI) sequences.
Marker
Schwann Cell Marker
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
MPZ
Purity
Immunogen affinity purified
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Western Blot reactivity reported in scientific literature (PMID: 22689911). Use in immunoprecipitation reported in scientific literature (PMID:26406915).
Theoretical MW
27 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB100-1607 in the following applications:
Human, Mouse reactivity reported in scientific literature (PMID: 26406915), Rat reactivity reported in scientific literature (PMID: 22511272).
Packaging, Storage & Formulations
Storage
Store at 4C in the dark.
Buffer
10mM PBS (0.9% isotonic, w/v, pH 7.2)
Preservative
0.02% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified
Notes
Chicken products cannot be exported to Canada.
Purification Notes After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 ug/ml. Finally, equal volumes of both of these affinitypurified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Storage Notes Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Alternate Names for Myelin Protein Zero Antibody
Charcot-Marie-Tooth neuropathy 1B
CHM
CMT1
CMT1B
CMT2I
CMT2J
CMT4E
CMTDI3
HMSNIB
MPP
Myelin peripheral protein
myelin protein P0
myelin protein zeroDSS
P0
Background
Myelin Protein Zero encodes a major structural protein of peripheral myelin. Mutations in this gene result in the autosomal dominant form of Charcot-Marie-Tooth disease type 1 as other polyneuropathies. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Su W, He X, Lin Z et al. Activation of P2X7R inhibits proliferation and promotes the migration and differentiation of Schwann cells Research Square 2023-10-26 (Immunocytochemistry/ Immunofluorescence, Mouse)
Malheiro A Investigating the effect of phosphodiesterase-4 (PDE4) inhibitors on Schwann cells myelination in a 3D regeneration model and in a hyperglycemia model Book 2021-06-02
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Product General Protocols
View specific protocols for Myelin Protein Zero Antibody (NB100-1607):
FAQs for Myelin Protein Zero Antibody (NB100-1607). (Showing 1 - 1 of 1 FAQs).
Does this antibody target the extracellular domain of the protein?
Two different peptides were used to raise this antibody: 1) the first peptide sequence is located in the amino-terminal half of the protein and is located within an "Immunoglobulin V-type" domain, which is extracellular. 2) The second peptide sequence is located near the C-terminus of the protein (cytoplasmic). The antibody recognizes MPZ protein, when both antigens are available but we do not know how strong would be the signal if only extracellular antigen is visible.
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![Immunocytochemistry/Immunofluorescence: Myelin Protein Zero Antibody [NB100-1607] - Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of an adult mouse. (Green) P0 concentration was 1:1000 dilution, followed by fluorescein-labeled goat anti-chicken IgY (1:500 dilution). Red is actin. Blue is DAPI nuclear counterstain.](http://images.novusbio.com/fullsize/Myelin-Protein-Zero-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1607-img0001.jpg "Immunocytochemistry/Immunofluorescence: Myelin Protein Zero Antibody [NB100-1607] - Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of an adult mouse. (Green) P0 concentration was 1:1000 dilution, followed by fluorescein-labeled goat anti-chicken IgY (1:500 dilution). Red is actin. Blue is DAPI nuclear counterstain.")
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![Immunohistochemistry: Myelin Protein Zero Antibody [NB100-1607] - In the image (a lower power tissue section through an adult sciatic nerve), Myelin Protein Zero (brown staining) can be seen in all of the myelinating Schwann cells.](http://images.novusbio.com/fullsize/Myelin-Protein-Zero-Antibody-Immunohistochemistry-NB100-1607-img0002.jpg "Immunohistochemistry: Myelin Protein Zero Antibody [NB100-1607] - In the image (a lower power tissue section through an adult sciatic nerve), Myelin Protein Zero (brown staining) can be seen in all of the myelinating Schwann cells.")
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