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SH3PX1 Antibody (OTI1B5)

Images

 
Western Blot: SH3PX1 Antibody (1B5) [NBP2-02609] Analysis of extracts (35ug) from 9 different cell lines by using anti-SH3PX1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: SH3PX1 Antibody (1B5) [NBP2-02609] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SH3PX1.
Immunohistochemistry-Paraffin: SH3PX1 Antibody (1B5) [NBP2-02609] - Staining of paraffin-embedded Human prostate tissue using anti-SH3PX1 mouse monoclonal antibody.
Flow Cytometry: SH3PX1 Antibody (1B5) [NBP2-02609] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SH3PX1 antibody, and then ...read more
Western Blot: SH3PX1 Antibody (1B5) [NBP2-02609] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH3PX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: SH3PX1 Antibody (1B5) [NBP2-02609] - Immunofluorescent staining of HeLa cells using anti-SH3PX1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SH3PX1 Antibody (1B5) [NBP2-02609] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SH3PX1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SH3PX1 Antibody (1B5) [NBP2-02609] - Staining of paraffin-embedded Human colon tissue using anti-SH3PX1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI1B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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SH3PX1 Antibody (OTI1B5) Summary

Immunogen
Full length human recombinant protein of human SNX9(NP_057308) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SNX9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000-2000
Theoretical MW
66.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SH3PX1 Antibody (OTI1B5)

  • MST155
  • MSTP155
  • Protein SDP1
  • SDP1
  • SH3 and PX domain-containing protein 1
  • SH3PX1WISP
  • SH3PXD3ASH3 and PX domain-containing protein SH3PX1
  • sorting nexin 9
  • sorting nexin-9
  • WiskSH3 and PX domain-containing protein 3A

Background

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like some family members, but does contain a SH3 domain near its N-terminus. This protein interacts with the cytoplasmic domains of the precursor but not the processed forms of a disintegrin and metalloprotease domain 9 and 15. This protein binds the beta-appendage domain of adaptor protein 2 and may function to assist adaptor protein 2 in its role at the plasma membrane. This protein interacts with activated Cdc42-associated kinase-2 to regulate the degradation of epidermal growth factor receptor protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SNX9
Entrez