Novus Biologicals products are now on bio-techne.com

Amphiphysin/AMPH Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining in human cerebral cortex and tonsil tissues using anti-AMPH antibody. Corresponding AMPH RNA-seq data are ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human cerebral cortex, colon, kidney and tonsil using Anti-AMPH antibody NBP1-87561 (A) shows similar ...read more
Western Blot: Amphiphysin/AMPH Antibody [NBP1-87561] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue
Immunocytochemistry/ Immunofluorescence: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human cell line U-251 MG shows localization to plasma membrane and cytosol.
Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human kidney.
Western Blot: Amphiphysin/AMPH Antibody [NBP1-87561] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue
Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human tonsil shows low expression as expected.
Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human colon.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Amphiphysin/AMPH Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EKVIPSVVIEPASNHEEEGENEITIGAEPKETTEDAAPPGPTSETPELATEQKPIQDPQPTPSAPAMGAADQLASAREA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AMPH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Amphiphysin/AMPH Protein (NBP1-87561PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Amphiphysin/AMPH Antibody

  • AMPH
  • AMPH1
  • amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen)
  • amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen)
  • amphiphysin I
  • Amphiphysin

Background

Amphiphysin, a neuronal protein first identified in chicken synaptic membranes, is the autoantigen of Stiff-Man Syndrome (SMS) associated with breast cancer. Patient autoantibodies have a distinct pattern of reactivity with amphiphysin, and the dominant autoepitope is located in its C-terminal region, which contains an SH3 domain (1). Amphiphysin is expressed in many neurons, certain endocrine cell types, and spermatocytes (2). study suggests a link between amphiphysin I expression in cancer and amphiphysin I autoimmunity. The enhanced expression of amphiphysin I in some forms of cancer supports the hypothesis that amphiphysin family members may play a role in the biology of cancer cells (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-22164
Species: Hu(-), Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
NBP2-67187
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC, IHC-P, IP, WB
PP-A9033A-00
Species: Hu
Applications: DirELISA, IHC, IP, WB
NB100-56094
Species: Hu
Applications: IHC, IHC-P, IP, WB
NBP1-00178
Species: Hu, Mu, Sh
Applications: Flow, ICC/IF, IHC, IHC-Fr, PEP-ELISA, WB
NBP1-46535
Species: Hu, Mu, Rb, Rt, Xp
Applications: ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, WB
NBP1-89102
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-88927
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-89396
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-50037
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
NBP3-35300
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
AF2685
Species: Hu
Applications: IHC, Simple Western, WB
NB300-109
Species: Ch, Dr, Hu, I, Ma, Mu, Rt
Applications: Dual ISH-IHC, ICC/IF, IHC-FrFl, IHC-WhMt, IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
2914-HT
Species: Hu
Applications: BA
NB100-1533
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
NBP1-33419
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF2086
Species: Hu, Mu, Rt
Applications: IHC, Simple Western, WB
AF4928
Species: Hu
Applications: CyTOF-ready, Flow, WB

Publications for Amphiphysin/AMPH Antibody (NBP1-87561) (0)

There are no publications for Amphiphysin/AMPH Antibody (NBP1-87561).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Amphiphysin/AMPH Antibody (NBP1-87561) (0)

There are no reviews for Amphiphysin/AMPH Antibody (NBP1-87561). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Amphiphysin/AMPH Antibody (NBP1-87561) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Amphiphysin/AMPH Products

Research Areas for Amphiphysin/AMPH Antibody (NBP1-87561)

Find related products by research area.

Blogs on Amphiphysin/AMPH

There are no specific blogs for Amphiphysin/AMPH, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Amphiphysin/AMPH Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol AMPH