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SH3PX1 Antibody (OTI1B5) - Azide and BSA Free

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Western Blot: SH3PX1 Antibody (OTI1B5) - Azide and BSA Free [NBP2-74158] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH3PX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH3PX1.
Western Blot: SH3PX1 Antibody (OTI1B5) - Azide and BSA Free [NBP2-74158] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH3PX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: SH3PX1 Antibody (OTI1B5) - Azide and BSA Free [NBP2-74158] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SH3PX1.
Immunohistochemistry: SH3PX1 Antibody (OTI1B5) - Azide and BSA Free [NBP2-74158] - Staining of paraffin-embedded Human colon tissue using anti-SH3PX1 mouse monoclonal antibody.
Flow Cytometry: SH3PX1 Antibody (OTI1B5) - Azide and BSA Free [NBP2-74158] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SH3PX1 ...read more
Western Blot: SH3PX1 Antibody (OTI1B5) - Azide and BSA Free [NBP2-74158] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SH3PX1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: SH3PX1 Antibody (OTI1B5) - Azide and BSA Free [NBP2-74158] - Staining of HeLa cells using anti-SH3PX1 mouse monoclonal antibody.
Immunohistochemistry: SH3PX1 Antibody (OTI1B5) - Azide and BSA Free [NBP2-74158] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SH3PX1 mouse monoclonal antibody.
Immunohistochemistry: SH3PX1 Antibody (OTI1B5) - Azide and BSA Free [NBP2-74158] - Staining of paraffin-embedded Human prostate tissue using anti-SH3PX1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI1B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74158AF350
Alexa Fluor 405
NBP2-74158AF405
Alexa Fluor 488
NBP2-74158AF488
Alexa Fluor 532
NBP2-74158AF532
Alexa Fluor 594
NBP2-74158AF594
Alexa Fluor 647
NBP2-74158AF647
Alexa Fluor 700
NBP2-74158AF700
Alexa Fluor 750
NBP2-74158AF750
Allophycocyanin
NBP2-74158APC
Allophycocyanin/Cy7
NBP2-74158APCCY7
Biotin
NBP2-74158B
CoraFluor 1
NBP2-74158CL1
DyLight 350
NBP2-74158UV
DyLight 405
NBP2-74158V
DyLight 488
NBP2-74158G
DyLight 550
NBP2-74158R
DyLight 594
NBP2-74158DL594
DyLight 650
NBP2-74158C
DyLight 680
NBP2-74158FR
DyLight 755
NBP2-74158IR
FITC
NBP2-74158F
HRP
NBP2-74158H
Janelia Fluor 525
NBP2-74158JF525
Janelia Fluor 549
NBP2-74158JF549
Janelia Fluor 646
NBP2-74158JF646
Janelia Fluor 669
NBP2-74158JF669
PE
NBP2-74158PE
PE/Atto594
NBP2-74158PEATT594
PE/Cy5.5
NBP2-74158PECY55
PE/Cy7
NBP2-74158PECY7
PerCP
NBP2-74158PCP
Unconjugated
NBP2-02609
mFluor Violet 450 SE
NBP2-74158MFV450
mFluor Violet 500 SE
NBP2-74158MFV500
mFluor Violet 610 SE
NBP2-74158MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-02609
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SH3PX1 Antibody (OTI1B5) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human SNX9(NP_057308) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SNX9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-2000
Theoretical MW
66.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for SH3PX1 Antibody (OTI1B5) - Azide and BSA Free

  • MST155
  • MSTP155
  • Protein SDP1
  • SDP1
  • SH3 and PX domain-containing protein 1
  • SH3PX1WISP
  • SH3PXD3ASH3 and PX domain-containing protein SH3PX1
  • sorting nexin 9
  • sorting nexin-9
  • WiskSH3 and PX domain-containing protein 3A

Background

The SH3PX1 (SNX1) gene is part of the sorting nexin family, thus plays a role in several states of intracellular trafficking. There is only one isoform at 595 amino acids long and at a mass of around 66 kDA. The SH3PX1 gene has been linked to choanal atresia, alcoholism, asthma, rabies, neuronitis, and wiskitt-aldrich syndrome. It is involved in clathrin derived and trans-Golgi network vesicle budding as well as membrane trafficking through interactions with other genes such as ITCH, DNM2, SYNJ1, ASAP1, and ADAM15.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol SNX9