eNOS Antibody - BSA Free

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Porcine, Sheep.

We have publications tested in 3 applications: Flow Cytometry, IF/IHC, WB.

Showing Publications 1 - 10 of 28. Show All 28 Publications. Collapse Publications.

Publications using NB300-500	Applications	Species
Han-Ying Wang, Hiroshi Takagi, Patrick N. Stoney, Anai Echeverria, Bernd Kuhn, Kuei-Sen Hsu, Tomoyuki Takahashi Anoxia-induced hippocampal LTP is regeneratively produced by glutamate and nitric oxide from the neuro-glial-endothelial axis iScience 2024-03-16 [PMID: 38591010]
Cosarderelioglu C, Kreimer S, Plaza-Rodriguez AI et al. Decoding Angiotensin Receptors: TOMAHAQ-Based Detection and Quantification of Angiotensin Type-1 and Type-2 Receptors Journal of the American Heart Association 2023-09-19 [PMID: 37681524] (WB)	WB
Lee S, Baek S, Lee Y et al. Ginsenoside Rg3-enriched Korean red ginseng extract attenuates Non-Alcoholic Fatty Liver Disease by way of suppressed VCAM-1 expression in liver sinusoidal endothelium Journal of Ginseng Research 2022-10-01 [PMID: 37252282] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
SuSnjara P, Mihaljevic Z, Stupin A et al. Consumption of nutritionally enriched hen eggs enhances endothelium-dependent vasodilation via cyclooygenases� metabolites in healthy young persons - A randomized study Research Square 2022-11-02 [PMID: 37049437] (WB, Human)	WB	Human
Caglar Cosarderelioglu, Lolita S Nidadavolu, Claudene J George, Ruth Marx-Rattner, Laura Powell, Qian-Li Xue, Jing Tian, Joy Salib, Esther S Oh, Luigi Ferrucci, Pervin Dincer, David A Bennett, Jeremy D Walston, Peter M Abadir Higher Angiotensin II Type 1 Receptor Levels and Activity in the Postmortem Brains of Older Persons with Alzheimer's Dementia. The journals of gerontology. Series A, Biological sciences and medical sciences 2022-04-05 [PMID: 34914835]
Fujita M, Tanaka N, Sakaguchi Y et al. Small caliber heparin loaded ultrafine microfiber woven graft achieved high patency rate in a preliminary study of canine carotid artery implantation JVS Vasc Sci 2021-10-07 [PMID: 34617041]
Patel PD, Chen YL, Kasetti RB et al. Impaired TRPV4-eNOS signaling in trabecular meshwork elevates intraocular pressure in glaucoma Proceedings of the National Academy of Sciences of the United States of America 2021-04-20 [PMID: 33853948]
Ricardo-da-Silva FY, Armstrong R, Vidal-Dos-Santos M Et al. 17 beta-Estradiol Treatment Protects Lungs Against Brain Death Effects in Female Rat Donor Transplantation 2021-04-01 [PMID: 33031230]
X Geng, K Yanagida, RG Akwii, D Choi, L Chen, Y Ho, B Cha, MR Mahamud, K Berman de, H Ichise, H Chen, J Wythe, CM Mikelis, T Hla, RS Srinivasan S1PR1 regulates the quiescence of lymphatic vessels by inhibiting laminar shear stress-dependent VEGF-C signaling JCI Insight, 2020-07-23;0(0):. 2020-07-23 [PMID: 32544090]
Ottolini M, Daneva Z, Chen YL et al. Mechanisms underlying selective coupling of endothelial Ca2+ signals with eNOS versus IK/SK channels in systemic and pulmonary arteries J. Physiol. (Lond.) 2020-05-28 [PMID: 32463112] (WB)	WB
Patel G, Fury W, Yang H et al. Molecular taxonomy of human ocular outflow tissues defined by single cell transcriptomics Proc Natl Acad Sci U S A 2020-05-23 [PMID: 32439707]
Sadhukhan R, Leung J. W. C, et al. Fractionated radiation suppresses Kruppel-like factor 2 pathway to a greater extent than by single exposure to the same total dose. Sci Rep 2020-05-07 [PMID: 32382091] (WB, Human)	WB	Human
Gao XX, Zhang QF, Zhu M et al. Effects of l-arginine on endometrial estrogen receptor alpha/beta and progesterone receptor expression in nutrient-restricted sheep Theriogenology 2019-07-25 [PMID: 31352175] (WB, Sheep)	WB	Sheep
CeYlan B, OzansoY M, kilic u et al. N-acetylcysteine suppresses colistimethate sodium-induced nephrotoxicity via activation of SOD2, eNOS, and MMP3 protein expressions. Ren Fail. 2018-11-01 [PMID: 30035652] (WB, Rat)	WB	Rat
Zhang L, Zheng H, Luo J et al. Inhibition of endothelial nitric oxide synthase reverses the effect of exercise on improving cognitive function in hypertensive rats. Hypertens. Res. 2018-03-22 [PMID: 29568075] (IF/IHC, Rat)	IF/IHC	Rat
Leao VF, Ferreira LLDM, Melo CM, Bonfleur ML. Taurine supplementation prevents endothelial dysfunction and attenuates structural changes in aortas from hypothalamic obese rats. Eur J Nutr. 2018-01-27 [PMID: 29374793] (WB, Rat)	WB	Rat
Nelson RK, Ya-Ping J, Gadbery J et al. Phospholipase D2 loss results in increased blood pressure via inhibition of the endothelial nitric oxide synthase pathway Sci Rep 2017-08-22 [PMID: 28831159] (WB, Mouse)	WB	Mouse
Yuki Hirano, Alex Choi, Masashi Tsuruta, Jen Erh Jaw, Yeni Oh, David Ngan, Konosuke Moritani, Yu-Wei Roy Chen, Sheena Tam, Yuexin Li, Dragos M Vasilescu, James C Hogg, Gordon Francis, Pascal Bernatchez, Shu-Fan Paul Man, Don D Sin Surfactant protein-D deficiency suppresses systemic inflammation and reduces atherosclerosis in ApoE knockout mice. Cardiovascular research 2018-05-21 [PMID: 28472244]
Oczkowicz G, Caban A, Budzinski G et al. Endothelial Nitric Oxide Synthase and Endothelin-1 Expression in the Early Post-Porcine Kidney Autotransplantation Period. Transplant. Proc. 2016-06-01 [PMID: 27496502] (WB, Porcine)	WB	Porcine
Andrukhova Olena, Slavic Svetlana, Odorfer Kathrin I et al. Experimental Myocardial Infarction Upregulates Circulating Fibroblast Growth Factor-23. Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research 2015-01-01 [PMID: 25858796] (WB, Mouse)	WB	Mouse
Oh YK, Bachar AR, Zacharias DG et al. Humanin Preserves Endothelial Function and Prevents Atherosclerotic Plaque Progression in Hypercholesterolemic ApoE Deficient Mice. Atherosclerosis;219(1):65-73. 2011-11-01 [PMID: 21763658]
Zhang H, Zha X, Zhang B et al. Dietary rumen-protected L-arginine or N-carbamylglutamate enhances placental amino acid transport and suppresses angiogenesis and steroid anabolism in underfed pregnant ewes Animal Nutrition 2023-08-01 (WB, Sheep)	WB	Sheep
Xia N, Horke S et al. Uncoupling of Endothelial Nitric Oxide Synthase in Perivascular Adipose Tissue of Diet-Induced Obese Mice. Arterioscler Thromb Vasc Biol 2016-01-01 [PMID: 26586660] (IF/IHC, Mouse)	IF/IHC	Mouse
Kurobe H, Maxfield MW, Tara S et al. Development of small diameter nanofiber tissue engineered arterial grafts PLoS ONE 2015-04-02 [PMID: 25830942] (IF/IHC, Mouse)	IF/IHC	Mouse
HOGBEN L. Risk of jaundice following transfusion with pooled plasma or serum. Br J Soc Med 1947-07-01 [PMID: 20253608]
Shirasuna K, Asahi T, Sasaki M et al. Distribution of arteriolovenous vessels, capillaries and eNOS expression in the bovine corpus luteum during the estrous cycle: a possible implication of different sensitivity by luteal phase to PGF(2alpha) in the increase of luteal blood flow. J Reprod Dev. 2010-02-01 [PMID: 19893278]
Wang M, Lung MA. Acetylcholine induces contractile and relaxant effects in canine nasal venous systems. Eur Respir J. 2006-10-01 [PMID: 16737988]
Guo FH, Comhair SA, Zheng S et al. Molecular mechanisms of increased nitric oxide (NO) in asthma: evidence for transcriptional and post-translational regulation of NO synthesis. J Immunol. 2000-06-01 [PMID: 10820280]
Show All 28 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Verified Customer	WB	Mouse	05/23/2017	View Summary Application Western Blot Sample Tested Mouse brain Species Mouse
	5 reviewed by: Verified Customer	IHC	Mouse	05/23/2017	View Summary Application Immunohistochemistry Sample Tested Mouse brain Species Mouse
Enlarge	5 reviewed by: Verified Customer	IHC-P	Mouse	02/22/2016	View Summary Application Immunohistochemistry-Paraffin Sample Tested Mouse Carotid Artery Species Mouse

Array NB300-500

• eNOS Antibodies
• eNOS ELISA Kits
• eNOS Lysate
• eNOS Protein
• eNOS ELISA