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![Western Blot: BIN1 Antibody [NBP1-89102] - Analysis in human cell lines Caco-2 and MCF-7 using anti-BIN1 antibody. Corresponding BIN1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP1-89102-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: BIN1 Antibody [NBP1-89102] - Analysis in human cell lines Caco-2 and MCF-7 using anti-BIN1 antibody. Corresponding BIN1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BIN1 Antibody [NBP1-89102] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89102-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89102] - Staining in human skeletal muscle and heart muscle tissues using anti-BIN1 antibody. Corresponding BIN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89102-img0012.jpg)
![Western Blot: BIN1 Antibody [NBP1-89102] - Analysis in control (vector only transfected HEK293T lysate) and BIN1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP1-89102-img0015.jpg)
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89102] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89102-img0006.jpg)
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89102] - Staining of human heart muscle shows low expression as expected.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89102-img0011.jpg)
![Western Blot: BIN1 Antibody [NBP2-15587] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with BIN1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP2-15587-img0009.jpg "Western Blot: BIN1 Antibody [NBP2-15587] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with BIN1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: BIN1 Antibody [NBP2-15587] - BIN1 antibody detects BIN1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BIN1 stained by BIN1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15587-img0008.jpg)
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP2-15587] - BIN1 antibody detects BIN1 protein at cytoplasm in human papillary adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human papillary adenocarcinoma. BIN1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP2-15587-img0005.jpg)
![Western Blot: BIN1 Antibody [NBP2-15587] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BIN1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP2-15587-img0006.jpg)
![Western Blot: BIN1 Antibody [NBP2-15587] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BIN1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP2-15587-img0007.jpg)
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP2-15587] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP2-15587-img0003.jpg)
![Western Blot: BIN1 Antibody (3B6F10) [NBP2-61714] - Analysis using BIN1 mAb against HEK293 (1) and BIN1 (AA: 189-398)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BIN1-Antibody-3B6F10-Western-Blot-NBP2-61714-img0005.jpg "Western Blot: BIN1 Antibody (3B6F10) [NBP2-61714] - Analysis using BIN1 mAb against HEK293 (1) and BIN1 (AA: 189-398)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: BIN1 Antibody (3B6F10) [NBP2-61714] - Analysis of paraffin-embedded cervical cancer tissues using BIN1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BIN1-Antibody-3B6F10-Immunohistochemistry-NBP2-61714-img0003.jpg)
![Flow Cytometry: BIN1 Antibody (3B6F10) [NBP2-61714] - Analysis of Hela cells using BIN1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BIN1-Antibody-3B6F10-Flow-Cytometry-NBP2-61714-img0002.jpg)
![Western Blot: BIN1 Antibody (3B6F10) [NBP2-61714] - Analysis using BIN1 mAb against human BIN1 (AA: 189-398) recombinant protein. (Expected MW is 47.1 kDa)](http://images.novusbio.com/fullsize/BIN1-Antibody-3B6F10-Western-Blot-NBP2-61714-img0004.jpg)
![Western Blot: BIN1 Antibody (3B6F10) [NBP2-61714] - Analysis using BIN1 mouse mAb against C2C12 (1), A431 (2), HEK293 (3), and MCF-7 (4) cell lysate.](http://images.novusbio.com/fullsize/BIN1-Antibody-3B6F10-Western-Blot-NBP2-61714-img0006.jpg)
![ELISA: BIN1 Antibody (3B6F10) [NBP2-61714] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/BIN1-Antibody-3B6F10-ELISA-NBP2-61714-img0001.jpg)
![Immunohistochemistry-Paraffin: BIN1 Antibody (2F11) [NBP2-21675] - Mouse Anti-BIN1 antibody. Tissue: skeletal muscle from BIN-1 wild type and null mouse. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. BIN-1 antibody for 1hr at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN1 as precipitated brown signal.](http://images.novusbio.com/fullsize/BIN1-Antibody-2F11-Immunohistochemistry-Paraffin-NBP2-21675-img0001.jpg "Immunohistochemistry-Paraffin: BIN1 Antibody (2F11) [NBP2-21675] - Mouse Anti-BIN1 antibody. Tissue: skeletal muscle from BIN-1 wild type and null mouse. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. BIN-1 antibody for 1hr at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN1 as precipitated brown signal.")
![Western Blot: BIN1 Antibody [NBP1-46170] - Detection of Human Bin1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Bin1 antibody NBP1-46170 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP1-46170-img0007.jpg "Western Blot: BIN1 Antibody [NBP1-46170] - Detection of Human Bin1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Bin1 antibody NBP1-46170 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-46170] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Bin1 used at a dilution of 1:1,000 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP1-46170-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: BIN1 Antibody [NBP1-46170] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Bin1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Bin1 was also immunoprecipitated by rabbit anti-Bin1 antibody NBP1-46169, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP1-46170-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BIN1 Antibody [NBP1-46170] - Detection of human Bin1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Bin1 antibody NBP1-46170 used for IP at 6 ug per reaction. Bin1 was also immunoprecipitated by another rabbit anti-Bin1 antibody. For blotting immunoprecipitated Bin1, NBP1-46170 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunoprecipitation-NBP1-46170-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: BIN1 Antibody (99F) [NBP2-21674] - Cells: C2C12 cells during growth or differentiation. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: BIN-1 (Exon 10 specific, 99F) monoclonal antibody. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN 1 as green fluorescent signal.](http://images.novusbio.com/fullsize/BIN1-Antibody-99F-Immunocytochemistry-Immunofluorescence-NBP2-21674-img0002.jpg "Immunocytochemistry/Immunofluorescence: BIN1 Antibody (99F) [NBP2-21674] - Cells: C2C12 cells during growth or differentiation. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: BIN-1 (Exon 10 specific, 99F) monoclonal antibody. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN 1 as green fluorescent signal.")
![Immunoprecipitation of Mouse anti-BIN1 antibody. Immunoprecipitates were separated by SDS-PAGE and visualized by fluorography. The differentiation-associated (Exon 10+) form of Bin1 is recognized by using Bin1 (Exon 10 specific, 99F) monoclonal antibody. Lane 1: BIN1 [35ug]. Primary antibody: Anti-BIN1 (Exon 10 specific, 99F) monoclonal antibody at 1:400 for overnight at 2-8C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted MW: 64.7 kDa. Observed MW: ~65 kDa for BIN-1.](http://images.novusbio.com/fullsize/nbp2-21674_mouse-monoclonal-bin1-antibody-99f-2352023180522.jpg)
![Western Blot: BIN1 Antibody (99D) [NBP2-21673] - Lane 1: Keratinocyte derived from Bin1 wild type mice. Lane 2: Keratinocyte derived from Bin1 null mice. Load: 35 ug per lane. BIN1 monoclonal Antibody. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBS, 0.4% Tween-20. Other band: non-specific.](http://images.novusbio.com/fullsize/BIN1-Antibody-99D-Western-Blot-NBP2-21673-img0001.jpg "Western Blot: BIN1 Antibody (99D) [NBP2-21673] - Lane 1: Keratinocyte derived from Bin1 wild type mice. Lane 2: Keratinocyte derived from Bin1 null mice. Load: 35 ug per lane. BIN1 monoclonal Antibody. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBS, 0.4% Tween-20. Other band: non-specific.")
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89103] - Staining in human skeletal muscle and skin tissues using anti-BIN1 antibody. Corresponding BIN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89103-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89103] - Staining in human skeletal muscle and skin tissues using anti-BIN1 antibody. Corresponding BIN1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: BIN1 Antibody [NBP1-89103] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells and glial cells.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-NBP1-89103-img0003.jpg)
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89103] - Staining of human skeletal muscle shows high expression.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89103-img0005.jpg)
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89103] - Staining of human skin shows low expression as expected.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89103-img0006.jpg)
![Western Blot: BIN1 Antibody (12A) [NBP2-21689] - Mouse Anti-BIN-1 Antibody. Lane 1: LNCap. Lane 2: WM1341D. Lane 3: WM164. Lane 4: WM983A. Lane 5: WM793. Lane 6: WM239A. Lane 7: WM35. Load: 35 ug per lane. BIN-1 antibody at 1:400 for overnight at 4C. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blocking Buffer overnight at 4C.](http://images.novusbio.com/fullsize/BIN1-Antibody-12A-Western-Blot-NBP2-21689-img0001.jpg "Western Blot: BIN1 Antibody (12A) [NBP2-21689] - Mouse Anti-BIN-1 Antibody. Lane 1: LNCap. Lane 2: WM1341D. Lane 3: WM164. Lane 4: WM983A. Lane 5: WM793. Lane 6: WM239A. Lane 7: WM35. Load: 35 ug per lane. BIN-1 antibody at 1:400 for overnight at 4C. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blocking Buffer overnight at 4C.")
![Western Blot: BIN1 Antibody (2C7) [H00000274-M03] - Analysis of BIN1 expression in transfected 293T cell line by BIN1 monoclonal antibody (M03), clone 2C7. Lane 1: BIN1 transfected lysatE (53 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BIN1-Antibody-2C7-Western-Blot-H00000274-M03-img0003.jpg "Western Blot: BIN1 Antibody (2C7) [H00000274-M03] - Analysis of BIN1 expression in transfected 293T cell line by BIN1 monoclonal antibody (M03), clone 2C7. Lane 1: BIN1 transfected lysatE (53 KDa). Lane 2: Non-transfected lysate.")
![ELISA: BIN1 Antibody (2C7) [H00000274-M03] - Detection limit for recombinant GST tagged BIN1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/BIN1-Antibody-2C7-ELISA-H00000274-M03-img0002.jpg)
![Western Blot: BIN1 Antibody (1H1) [H00000274-M01] - Analysis of BIN1 expression in transfected 293T cell line by BIN1 monoclonal antibody (M01), clone 1H1.Lane 1: BIN1 transfected lysate(53 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BIN1-Antibody-1H1-Western-Blot-H00000274-M01-img0003.jpg "Western Blot: BIN1 Antibody (1H1) [H00000274-M01] - Analysis of BIN1 expression in transfected 293T cell line by BIN1 monoclonal antibody (M01), clone 1H1.Lane 1: BIN1 transfected lysate(53 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: BIN1 Antibody (1H1) [H00000274-M01] - BIN1 monoclonal antibody (M01), clone 1H1 Analysis of BIN1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/BIN1-Antibody-1H1-Western-Blot-H00000274-M01-img0002.jpg)
![Western Blot: BIN1 Overexpression Lysate (Adult Normal) [NBP2-09976] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BIN1.](http://images.novusbio.com/fullsize/BIN1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09976-img0001.jpg "Western Blot: BIN1 Overexpression Lysate (Adult Normal) [NBP2-09976] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BIN1.")