CD35 Antibody (E11)

The clone E11 antibody reacts with CD35, also known as complement receptor 1 (CR1), a 210-220 kDa protein (Hogg et al, 1985). CD35 is present on a subpopulation of T lymphocytes (~5%) which are relatively low in CD3 expression. CD35 is also expressed on CD20 positive B lymphocytes, CD14 positive monocytes, and on poly-morphonuclear leukocytes. The primary function of CD35 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. In Immunohistochemical staining (IHC) the CD35 antibody strongly labels the follicular dendritic cells (FDCs) and follicular dendritic cell sarcoma. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. The antibody is highly specific for CD35 and does not cross react with CD21 (CR2). Furthermore, this CD35 antibody does not block CD35 activity because it recognizes a CD35 epitope distal to the C3b binding site. The antibody has been widely cited in the literature. Examples include its use in antibody panels for immunophenotyping normal bone marrow stem cells by flow cytometry (Laranjeira et al, 2012) and characterizing infections in rheumatoid arthritis patients (Moduko et al, 2012). The CD35 antibody has also been used along with CD23 and CD21 antibodies for identifying FDCs in reactive and neoplastic lymphoid tissue by IHC (Bagdi et al, 2001). Furthermore, the CD35 antibody showed than tonsil-derived mesenchymal progenitor cells acquire an FDC-like phenotype following cytokine stimulation (Cho et al, 2012), and that oncolytic measles viral infection upregulates CD35 (Zhang et al, 2012). Additional publications using the CD35 antibody can be identified through a key word on scholar.google.com.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Monkey.

We have publications tested in 2 applications: IHC-P, ISH.

Showing Publications 1 - 6 of 6.

Publications using NBP2-29460	Applications	Species
Broeckel RM, Feldmann F, McNally KL et al. A pigtailed macaque model of Kyasanur Forest disease virus and Alkhurma hemorrhagic disease virus pathogenesis PLoS pathogens 2021-12-01 [PMID: 34855915] (ISH, Monkey)	ISH	Monkey
Dave Rajnish S, Sharma Ravi K, Muir Roshell R et al FDC:TFH Interactions within Cervical Lymph Nodes of SIV-Infected Rhesus Macaques. J Neuroimmune Pharmacol. 2018-06-01 [PMID: 29288344] (IHC-P, Monkey) Details: Citation using the HRP version of this antibody.	IHC-P	Monkey
Cho KA, Kim JY, Kim HS et al. Tonsil-derived mesenchymal progenitor cells acquire a follicular dendritic cell phenotype under cytokine stimulation. Cytokine. 2012-08-01 [PMID: 22578801]
Mokuda S, Doi O, Takasugi K. Simultaneous quantitative analysis of the expression of CD64 and CD35 on neutrophils as markers to differentiate between bacterial and viral infections in patients with rheumatoid arthritis. Mod Rheumatol. 2012-09-01 [PMID: 22237765]
Zhang Y, Patel B, Dey A et al. Attenuated, oncolytic, but not wild-type measles virus infection has pleiotropic effects on human neutrophil function. J Immunol. 2012-02-01 [PMID: 22180616]
Bagdi E, Krenacs L, Krenacs T et al. Follicular dendritic cells in reactive and neoplastic lymphoid tissues: a reevaluation of staining patterns of CD21, CD23, and CD35 antibodies in paraffin sections after wet heat-induced epitope retrieval. Appl Immunohistochem Mol Morphol. 2001-06-01 [PMID: 11396628]

Array NBP2-29460

• CD35 Antibodies
• CD35 ELISA Kits
• CD35 Proteins
• CD35 Proteins and Enzyme