![Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43) [NB500-330] - Staining of human placenta (paraffin sections) using anti-CD59 (MEM-43).](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Immunohistochemistry-Paraffin-NB500-330-img0009.jpg "Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43) [NB500-330] - Staining of human placenta (paraffin sections) using anti-CD59 (MEM-43).")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, IHC, IP, B/N, CyTOF-ready |
| Clone | MEM-43 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Clone MEM-43 was used by HLDA to establish CD designation. |
| Immunogen | Thymocytes and T lymphocytes (NM_000611.5). |
| Specificity | The antibody MEM-43 reacts with well defined epitope (W40, R-53) on CD59 (Protectin), an 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD59 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in blocking / neutralizing reported in scientific literature (PMID 24909955). CD59 antibody validated for IHC-F from a verified customer review. This antibody is CyTOF ready. |
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| Theoretical MW | 20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.9% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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Enlarge |
reviewed by:
Jan Martinek |
Immunofluorescence-Frozen | Human | 11/15/2018 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for CD59 Antibody (NB500-330)Find related products by research area.
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| Jan Martinek 11/15/2018 |
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| Application: | Immunofluorescence-Frozen | |
| Species: | Human |
| Gene Symbol | CD59 |
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) Pacific BlueTM.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Flow-Cytometry-NB500-330-img0007.jpg "Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) Pacific BlueTM.")
![Immunohistochemistry-Frozen: CD59 Antibody (MEM-43) [NB500-330] - Human tonsil frozen tissue section stained for CD59 (red) and counterstained with DAPI (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Immunohistochemistry-Frozen-NB500-330-img0008.jpg "Immunohistochemistry-Frozen: CD59 Antibody (MEM-43) [NB500-330] - Human tonsil frozen tissue section stained for CD59 (red) and counterstained with DAPI (blue). Image from verified customer review.")
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Analysis using the PE conjugate of NB500-330. Surface staining of human peripheral blood cells with anti-human CD59 (MEM-43) PE.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Flow-Cytometry-NB500-330-img0002.jpg "Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Analysis using the PE conjugate of NB500-330. Surface staining of human peripheral blood cells with anti-human CD59 (MEM-43) PE.")
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Using the PE direct conjugate Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) PE.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Flow-Cytometry-NB500-330-img0004.jpg "Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Using the PE direct conjugate Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) PE.")
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Using the Allophycocyanin direct conjugate Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) APC.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Flow-Cytometry-NB500-330-img0003.jpg "Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Using the Allophycocyanin direct conjugate Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) APC.")
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) FITC.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Flow-Cytometry-NB500-330-img0005.jpg "Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) FITC.")
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Flow-Cytometry-NB500-330-img0006.jpg "Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) biotin / streptavidin-APC.")
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