Complement Factor H Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Factor H is a 155 kDa glycoprotein that provides critical negative regulation of the alternative pathway complement cascade. It is secreted by Kupffer cells, hepatocytes, vascular endothelial cells, and platelets and circulates in the serum at high concentration (1). Factor H is composed of 20 SCR (short consensus repeats), each of which consists of approximately 60 amino acids with four invariant Cys residues (2). Factor H interacts with cell surface polyanions including heparin and sialoglycoproteins (3-6), and immobilized Factor H supports the CD11b/CD18 integrin-dependent adhesion of neutrophils (7). It prevents local complement activation by sequestering complement component C3b, accelerating the decay of C3 and C5 convertases, and functioning as a cofactor for the C3b inactivator, Factor I (1, 3, 6, 8). This recombinant protein corresponds to SCR15-20 which encompass the primary binding sites for heparin and C3b as well as for the peptide hormone adrenomedullin (4, 9-11). Within SCR15-20, mouse Factor H shares 60% and 80% amino acid sequence identity with human and rat Factor H, respectively. Dozens of mutations clustered in SCR15-20 are associated with atypical hemolytic uremic syndrome, a disorder characterized by anemia, thrombocytopenia, and renal failure (12). Binding of Factor H to tumor cell-associated dentin matrix protein 1, bone sialoprotein, or osteopontin results in the protection of that cell from complement mediated lysis (13, 14). A variety of pathogenic microbes also express Factor H binding molecules that interfere with immune clearance of the infection (15).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.

Showing Publications 1 - 4 of 4.

Publications using AF4999	Applications	Species
Nicole Schäfer, Anas Rasras, Delia M. Ormenisan, Sabine Amslinger, Volker Enzmann, Herbert Jägle et al. Complement Factor H-Related 3 Enhanced Inflammation and Complement Activation in Human RPE Cells Frontiers in Immunology 2021-11-08 [PMID: 34819935]
Anne Enzbrenner, Rahel Zulliger, Josef Biber, Ana Maria Quintela Pousa, Nicole Schäfer, Corinne Stucki et al. Sodium Iodate-Induced Degeneration Results in Local Complement Changes and Inflammatory Processes in Murine Retina International Journal of Molecular Sciences 2021-08-26 [PMID: 34502128]
Talat M, Daniel G, Deborah L et al. Gain-of-function factor H-related 5 protein impairs glomerular complement regulation resulting in kidney damage. Proc Natl Acad Sci U S A. 2021-03-30 [PMID: 33753502]
LD Halder, EAH Jo, MZ Hasan, M Ferreira-G, T Krüger, M Westermann, DI Palme, G Rambach, N Beyersdorf, C Speth, ID Jacobsen, O Kniemeyer, B Jungnickel, PF Zipfel, C Skerka Immune modulation by complement receptor 3-dependent human monocyte TGF-beta1-transporting vesicles Nat Commun, 2020-05-11;11(1):2331. 2020-05-11 [PMID: 32393780] (Western Blot, Human)	Western Blot	Human

Array AF4999

• Complement Factor H Antibodies
• Complement Factor H Antibody Pair
• Complement Factor H ELISA Kits
• Complement Factor H Lysate
• Complement Factor H Proteins
• Complement Factor H Proteins and Enzymes
• Complement Factor H ELISA