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CD46 Antibody [Unconjugated]

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Western blot shows lysate of human skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD46 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2005) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD46 at approximately 50-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysate of human skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD46 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2005) followed by HRP-conjugated Anti-Goat IgG ...read more
CD46 was detected in immersion fixed paraffin-embedded sections of human skin using 1.7 µg/mL Goat Anti-Human CD46 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2005) overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF2005
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2005-SP
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CD46 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD46
Cys35-Asn328
Accession # NP_722548
Specificity
Detects human CD46 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD46
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF2005 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD46 Antibody [Unconjugated]

  • AHUS2
  • CD46 antigen
  • CD46 molecule, complement regulatory protein
  • CD46
  • MCP
  • membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen)
  • MIC10
  • TLXcomplement regulatory protein
  • trophoblast leucocyte common antigen
  • Trophoblast leukocyte common antigen
  • trophoblast-lymphocyte cross-reactive antigen

Background

CD46, also known as MCP (complement membrane cofactor protein), is a type I membrane protein that has cofactor activity for inactivation of complement components C3b and C4b by serum factor I. CD46 is widely distributed in white blood cells, platelets, epithelial cells and fibroblasts. At least fourteen different transcript variants encoding different isoforms have been reported for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD46 Antibody (AF2005)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Flow Cytometry, ICC, Western Blot.


Filter By Application
Flow Cytometry
(2)
ICC
(1)
Western Blot
(1)
All Applications
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Human
(4)
Mouse
(1)
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Showing Publications 1 - 5 of 5.
Publications using AF2005 Applications Species
M Kschonsak, MC Johnson, R Schelling, EM Green, L Rougé, H Ho, N Patel, C Kilic, E Kraft, CP Arthur, AL Rohou, L Comps-Agra, N Martinez-M, L Perez, J Payandeh, C Ciferri Structural basis for HCMV Pentamer receptor recognition and antibody neutralization Science Advances, 2022-03-11;8(10):eabm2536. 2022-03-11 [PMID: 35275719] (Flow Cytometry, Human) Flow Cytometry Human
J Fisher, OE Sørensen, AHA Abu-Humaid Using chemiluminescence imaging of cells (CLIC) for relative protein quantification Sci Rep, 2020-10-26;10(1):18280. 2020-10-26 [PMID: 33106566] (ICC, Human) ICC Human
Martinez-Martin N, Marcandalli J, Huang C, Arthur C, Perotti M, Foglierini M, Ho H, Dosey A, Shriver S, Payandeh J, Leitner A, Lanzavecchia A, Perez L, Ciferri C An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor. Cell, 2018-07-26;174(5):1158-1171.e19. 2018-07-26 [PMID: 30057110] (Flow Cytometry, Human) Flow Cytometry Human
M Richter, R Yumul, K Saydaminov, H Wang, M Gough, A Baldessari, R Cattaneo, F Lee, CH Wang, H Jang, A Astier, A Gopal, D Carter, A Lieber Preclinical safety, pharmacokinetics, pharmacodynamics, and biodistribution studies with Ad35K++ protein: a novel rituximab cotherapeutic Mol Ther Methods Clin Dev, 2016-03-30;5(0):16013. 2016-03-30 [PMID: 27069950] (Mouse) Mouse
Greig JA, Buckley SM, Waddington SN, Parker AL, Bhella D, Pink R, Rahim AA, Morita T, Nicklin SA, McVey JH, Baker AH Influence of coagulation factor x on in vitro and in vivo gene delivery by adenovirus (Ad) 5, Ad35, and chimeric Ad5/Ad35 vectors. Mol. Ther., 2009-07-14;17(10):1683-91. 2009-07-14 [PMID: 19603000] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol CD46
Uniprot