I am interested in developing a surrogate ADCC assay (FC gamma III a binding assay) for which, I would require a CD16 Recombinant Protein as an antigen and Anti-Human IgG (Fc specific) as a secondary antibody as I am planning to perform this assay in an ELISA format. With this brief description, could you please suggest me the right CD16 Recombinant Protein among the three listed products in your web site (www.novusbio.com/proteins/cd16). Appreciate if you can share a reference article, where the use of this protein is cited.

Name: Recombinant Human Fc gamma RIIIA/CD16a Protein, CF
Brand: Novus Biologicals
SKU: 4325-FC
Price: 434 USD

The right CD16 protein for you depends on what sort of functionality you require for your assay. H00002214-P01 is made by a Taiwanese company called Abnova and we distribute it for them. They do not test the activity of their recombinant proteins. The proteins are produced in a cell-free wheat germ system with an N-terminal 26kDa GST-tag. Due to this expression system, in our experience, the proteins do not always undergo the same folding and post-transnational modifications they might undergo in an endogenous cell. Because of this, we do not recommend this protein for use in functional assays and do not guarantee them for this use. They are mainly intended for use in Western Blots and ELISA as a positive control with their antibody. NBP1-98908 is produced in E. coli and contains a His-tag and it also not guaranteed for functionality but may be due to its E. coli expression system. H00002214-G01 is also an Abnova and is made in their cell-free wheat germ expression system with proprietary liposome technology. This is a recently developed system by them and is intended to help the proteins fold and maintain functionality. Which protein would be best for you depends on what you require for your assay. Here is a link to our antibodies to the Fc region of human IgG. The best antibody for you will depend on what you would like to use for detection.

Recombinant Human Fc gamma RIIIA/CD16a Protein, CF (4325-FC): Novus Biologicals

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	Binding Activity
Format	Carrier-Free

Additional Information	Analyzed by SEC-MALS, Isoform V176, His-tag
Details of Functionality	Measured by its ability to bind human IgG with an estimated K_d <50 nM.
Source	Mouse myeloma cell line, NS0-derived human Fc gamma RIIIA/CD16a protein Gly17-Gln208, with a C-terminal 6-His tag
Accession #	AAH17865
N-terminal Sequence	Gly17
Protein/Peptide Type	Recombinant Proteins
Gene	FCGR3A
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note	<0.10 EU per 1 μg of the protein by the LAL method.

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS.
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions	Reconstitute at 100 μg/mL in sterile PBS.

Publications using 4325-FC	Applications	Species
Pascual-Pasto, G;McIntyre, B;Hines, MG;Giudice, AM;Garcia-Gerique, L;Hoffmann, J;Mishra, P;Matlaga, S;Lombardi, S;Shraim, R;Schürch, PM;Yarmarkovich, M;Hofmann, TJ;Alikarami, F;Martinez, D;Tsang, M;Gil-de-Gómez, L;Spear, TT;Bernt, KM;Wolpaw, AJ;Dimitrov, DS;Li, W;Bosse, KR; CAR T-cell-mediated delivery of bispecific innate immune cell engagers for neuroblastoma Nature communications 2024-08-21 [PMID: 39164224] (Bioassay, Human)	Bioassay	Human
Sun, H;Yang, M;Lai, H;Neupane, B;Teh, AY;Jugler, C;Ma, JK;Steinkellner, H;Bai, F;Chen, Q; A Dual-Approach Strategy to Optimize the Safety and Efficacy of Anti-Zika Virus Monoclonal Antibody Therapeutics Viruses 2023-05-11 [PMID: 37243242] (Surface Plasmon Resonance (SPR), N/A)	Surface Plasmon Resonance (SPR)	N/A
JA Torchia, AH Tavares, LS Carstensen, DY Chen, J Huang, T Xiao, S Mukherjee, PM Reeves, H Tu, AE Sluder, B Chen, DN Kotton, RA Bowen, M Saeed, MC Poznansky, GJ Freeman Optimized ACE2 decoys neutralize antibody-resistant SARS-CoV-2 variants through functional receptor mimicry and treat infection in vivo Science Advances, 2022-12-07;8(49):eabq6527. 2022-12-07 [PMID: 36475798] (ELISA Capture, Human)	ELISA Capture	Human
TA Bates, P Lu, YJ Kang, D Schoen, M Thornton, SK McBride, C Park, D Kim, WB Messer, ME Curlin, FG Tafesse, LL Lu BNT162b2-induced neutralizing and non-neutralizing antibody functions against SARS-CoV-2 diminish with age Cell Reports, 2022-10-05;41(4):111544. 2022-10-05 [PMID: 36252569] (Bioassay, Human)	Bioassay	Human
Y Kitanaga, D Yamajuku, S Kubo, K Nakamura, M Maeda, M Seki, Y Kaneko, F Kinugasa, T Morokata, Y Kondo, H Yoshinari, S Nakayamada, T Sumida, Y Tanaka Discovery of a novel Igbeta and FcgammaRIIB cross-linking antibody, ASP2713, and its potential application in the treatment of systemic lupus erythematosus International immunopharmacology, 2021-11-12;101(0):108343. 2021-11-12 [PMID: 34781122] (ELISA Capture, Human)	ELISA Capture	Human
B Vanhove, O Duvaux, J Rousse, PJ Royer, G Evanno, C Ciron, E Lheriteau, L Vacher, N Gervois, R Oger, Y Jacques, S Conchon, A Salama, R Duchi, I Lagutina, A Perota, P Delahaut, M Ledure, M Paulus, RT So, CK Mok, R Bruzzone, M Bouillet, S Brouard, E Cozzi, C Galli, D Blanchard, JM Bach, JP Soulillou High neutralizing potency of swine glyco-humanized polyclonal antibodies against SARS-CoV-2 European Journal of Immunology, 2021-03-02;0(0):. 2021-03-02 [PMID: 33576494] (Bioassay, Porcine)	Bioassay	Porcine
J Domján, P Vass, E Hirsch, E Szabó, E Pantea, SK Andersen, T Vigh, G Verreck, G Marosi, ZK Nagy Monoclonal antibody formulation manufactured by high-speed electrospinning Int J Pharm, 2020-11-04;0(0):120042. 2020-11-04 [PMID: 33157211] (Bioassay, Human)	Bioassay	Human
PA Blundell, D Lu, M Wilkinson, A Dell, S Haslam, RJ Pleass Insertion of N-Terminal Hinge Glycosylation Enhances Interactions of the Fc Region of Human IgG1 Monomers with Glycan-Dependent Receptors and Blocks Hemagglutination by the Influenza Virus J. Immunol., 2019-01-25;0(0):. 2019-01-25 [PMID: 30683699] (ELISA (Capture), Surface Plasmon Resonance, Human)	ELISA (Capture), Surface Plasmon Resonance	Human
G Wozniak-Kn, G Stadlmayr, JW Perthold, K Stadlbauer, M Gotsmy, S Becker, F Rüker An antibody with Fab-constant domains exchanged for a pair of CH3 domains PLoS ONE, 2018-04-09;13(4):e0195442. 2018-04-09 [PMID: 29630643] (Bioassay)	Bioassay
F Arce Varga, AJS Furness, K Litchfield, K Joshi, R Rosenthal, E Ghorani, I Solomon, MH Lesko, N Ruef, C Roddie, JY Henry, L Spain, A Ben Aissa, A Georgiou, YNS Wong, M Smith, D Strauss, A Hayes, D Nicol, T O'Brien, L Mårtensson, A Ljungars, I Teige, B Frendéus, M Pule, T Marafioti, M Gore, J Larkin, S Turajlic, C Swanton, KS Peggs, SA Quezada Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies Cancer Cell, 2018-03-22;0(0):. 2018-03-22 [PMID: 29576375] (Surface Plasmon Resonance, Human)	Surface Plasmon Resonance	Human
C Lee, M Jeong, JJ Lee, S Seo, SC Cho, W Zhang, O Jaquez Glycosylation profile and biological activity of Remicadeï¿½ compared with Flixabiï¿½ and Remsimaï¿½ MAbs, 2017-06-22;0(0):0. 2017-06-22 [PMID: 28640663] (Binding Assay)	Binding Assay
C Le Clorenn, H Bazin, O Dubreuil, C Larbouret, C Ogier, Y Lazrek, V Garambois, MA Poul, P Mondon, JM Barret, G Mathis, JF Prost, A Pèlegrin, T Chardès Neuregulin 1 allosterically enhances the anti-tumor effects of the non-competing anti-HER3 antibody 9F7-F11 by increasing its binding to HER3 Mol. Cancer Ther., 2017-05-15;0(0):. 2017-05-15 [PMID: 28507002] (Bioassay, Human)	Bioassay	Human
Lohse S, Meyer S, Meulenbroek L, Jansen J, Nederend M, Kretschmer A, Klausz K, Moginger U, Derer S, Rosner T, Kellner C, Schewe D, Sondermann P, Tiwari S, Kolarich D, Peipp M, Leusen J, Valerius T An Anti-EGFR IgA That Displays Improved Pharmacokinetics and Myeloid Effector Cell Engagement In Vivo. Cancer Res, 2015-12-03;76(2):403-17. 2015-12-03 [PMID: 26634925] (Surface Plasmon Resonance)	Surface Plasmon Resonance
Yang Z, Ramsey J, Hamza T, Zhang Y, Li S, Yfantis H, Lee D, Hernandez L, Seghezzi W, Furneisen J, Davis N, Therien A, Feng H Mechanisms of protection against Clostridium difficile infection by the monoclonal antitoxin antibodies actoxumab and bezlotoxumab. Infect Immun, 2014-12-08;83(2):822-31. 2014-12-08 [PMID: 25486992] (Bioassay, Human)	Bioassay	Human
Anikeeva N, Steblyanko M, Fayngerts S, Kopylova N, Marshall D, Powers G, Sato T, Campbell K, Sykulev Y Integrin receptors on tumor cells facilitate NK cell-mediated antibody-dependent cytotoxicity. Eur J Immunol, 2014-06-05;44(8):2331-9. 2014-06-05 [PMID: 24810893] (Bioassay, Human)	Bioassay	Human
Xie J, Yamniuk A, Borowski V, Kuhn R, Susulic V, Rex-Rabe S, Yang X, Zhou X, Zhang Y, Gillooly K, Brosius R, Ravishankar R, Waggie K, Mink K, Price L, Rehfuss R, Tamura J, An Y, Cheng L, Abramczyk B, Ignatovich O, Drew P, Grant S, Bryson J, Suchard S, Salter-Cid L, Nadler S, Suri A Engineering of a novel anti-CD40L domain antibody for treatment of autoimmune diseases. J Immunol, 2014-03-26;192(9):4083-92. 2014-03-26 [PMID: 24670803] (Bioassay, Human)	Bioassay	Human
Lajoie L, Congy-Jolivet N, Bolzec A, Gouilleux-Gruart V, Sicard E, Sung H, Peiretti F, Moreau T, Vie H, Clemenceau B, Thibault G ADAM17-mediated shedding of FcgammaRIIIA on human NK cells: identification of the cleavage site and relationship with activation. J Immunol, 2013-12-13;192(2):741-51. 2013-12-13 [PMID: 24337742] (Bioassay, Human)	Bioassay	Human
Franco A, Damdinsuren B, Ise T, Dement-Brown J, Li H, Nagata S, Tolnay M Human Fc receptor-like 5 binds intact IgG via mechanisms distinct from those of Fc receptors. J Immunol, 2013-04-24;190(11):5739-46. 2013-04-24 [PMID: 23616577] (Surface Plasmon Resonance (SPR, Human)	Surface Plasmon Resonance (SPR	Human
Boltz A, Piater B, Toleikis L, Guenther R, Kolmar H, Hock B Bi-specific Aptamers Mediating Tumor Cell Lysis. J. Biol. Chem., 2011-04-29;286(24):21896-905. 2011-04-29 [PMID: 21531729] (EMSA, Human)	EMSA	Human
von Horsten HH, Ogorek C, Blanchard V, Demmler C, Giese C, Winkler K, Kaup M, Berger M, Jordan I, Sandig V Production of non-fucosylated antibodies by co-expression of heterologous GDP-6-deoxy-D-lyxo-4-hexulose reductase. Glycobiology, 2010-07-15;20(12):1607-18. 2010-07-15 [PMID: 20639190] (Bioassay, Human)	Bioassay	Human
Wei Y, Li C, Huang W, Li B, Strome S, Wang LX Glycoengineering of human IgG1-Fc through combined yeast expression and in vitro chemoenzymatic glycosylation. Biochemistry, 2008-09-05;47(39):10294-304. 2008-09-05 [PMID: 18771295] (Surface Plasmon Resonance, Human)	Surface Plasmon Resonance	Human
Show All 21 Publications. Collapse Publications.

Recombinant Human Fc gamma RIIIA/CD16a Protein, CF

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Gene Symbol	FCGR3A
Uniprot	AAH17865()