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ARX Antibody

 (11 Publications)
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Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma cell line, U-118-MG human glioblastoma/astrocytoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ARX at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma cell line, U-118-MG human glioblastoma/astrocytoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Sheep ...read more
ARX was detected in immersion fixed U-87 MG human glioblastoma/astrocytoma cell line using Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) at 10 µg/mL for 3 hours at room ...read more
ARX was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) at 15 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF7068-SP
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ARX Antibody Summary

Immunogen
E. coli-derived recombinant human ARX
Ser2-Ala100
Accession # Q96QS3
Specificity
Detects human ARX in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ARX
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
AF7068 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ARX Antibody

  • aristaless related homeobox
  • Aristaless-related homeobox
  • aristaless-related homeobox, X-linked
  • ARX
  • cancer/testis antigen 121
  • CT121
  • EIEE1
  • homeobox protein ARX
  • ISSX
  • ISSXmental retardation, X-linked 36
  • mental retardation, X-linked 29
  • mental retardation, X-linked 32
  • mental retardation, X-linked 33
  • mental retardation, X-linked 38
  • mental retardation, X-linked 43
  • mental retardation, X-linked 54
  • mental retardation, X-linked 76
  • mental retardation, X-linked 87
  • MRX29
  • MRX32
  • MRX33
  • MRX36
  • MRX38
  • MRX43
  • MRX54
  • MRX76
  • MRX87
  • MRXS1
  • PRTS

Background

ARX (Aristaless-Related homeoboX protein) is a 56-62 kDa member of the aristaless subfamily, paired homeobox family of proteins. In the nervous system, it is expressed in embryonic telencephalon plus adult cortical GABAergic neurons and differentiating neuronal stem cells; in the pancreas, it promotes the differentiation of alpha -cells at the expense of beta -cells. Human ARX is a 562 amino acid (aa) transcriptional repressor. It contains an octapeptide motif that binds Groucho proteins (aa 27‑34), an NLS (aa 82-89), one Ala-rich region (aa 100-155), an acidic/Glu-rich region (aa 224-253), a DNA-binding homeodomain with an embedded NLS (aa 328‑387), one Pro-rich segment that binds CtBP1 (aa 395-459) and a C-terminal Aristaless domain (aa 530-543). There are mutations that show poly-Ala expansions in the N-terminus (aa 115-155), often associated with developmental abnormalities. Over aa 1-100, human ARX shares 97% aa identity with mouse ARX.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ARX Antibody (AF7068)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC, IHC-P.


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(1)
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(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF7068 Applications Species
Jingcheng Zhang, Rui Jiang, Xiafei Hong, Huanwen Wu, Xianlin Han, Wenming Wu Metastatic insulinoma: exploration from clinicopathological signatures and genetic characteristics Frontiers in Oncology 5/12/2023 [PMID: 37251916]
Sara J. Knowles, April M. Stafford, Tariq Zaman, Kartik Angara, Michael R. Williams, Jason M. Newbern et al. Distinct hyperactive RAS/MAPK alleles converge on common GABAergic interneuron core programs Development 2023-05-15 [PMID: 37166243]
Tincy Simon, Pamela Riemer, Armin Jarosch, Katharina Detjen, Annunziata Di Domenico, Felix Bormann et al. DNA methylation reveals distinct cells of origin for pancreatic neuroendocrine carcinomas and pancreatic neuroendocrine tumors Genome Medicine 2022-03-01 [PMID: 35227293]
Xiao-Qing Dai, Joan Camunas-Soler, Linford J.B. Briant, Theodore dos Santos, Aliya F. Spigelman, Emily M. Walker et al. Heterogenous impairment of alpha cell function in type 2 diabetes is linked to cell maturation state Cell Metabolism 2022-02-01 [PMID: 35108513]
Rachana Haliyur, John T Walker, May Sanyoura, Conrad V Reihsmann, Shristi Shrestha, Radhika Aramandla et al. Integrated Analysis of the Pancreas and Islets Reveals Unexpected Findings in Human Male With Type 1 Diabetes Journal of the Endocrine Society 2021-12-01 [PMID: 34870058]
Annunziata Di Domenico, Christodoulos P. Pipinikas, Renaud S. Maire, Konstantin Bräutigam, Cedric Simillion, Matthias S. Dettmer et al. Epigenetic landscape of pancreatic neuroendocrine tumours reveals distinct cells of origin and means of tumour progression Communications Biology 2020-12-07 [PMID: 33288854]
X Shi, W Lin, X Gao, W Xie, JA Golden, T Tao Identification and validation of the phosphorylation sites on Aristaless-related homeobox protein Biosci. Rep., 2020-07-31;40(7):. 2020-07-31 [PMID: 32608477] (Human) Human
N Moya, A Shahryari, I Burtscher, J Beckenbaue, M Bakhti, H Lickert Generation of a homozygous ARX nuclear CFP (ARXnCFP/nCFP) reporter human iPSC line (HMGUi001-A-4) Stem Cell Res, 2020-06-09;46(0):101874. 2020-06-09 [PMID: 32544857] (ICC, Human) ICC Human
P Seiron, A Wiberg, E Kuric, L Krogvold, FL Jahnsen, K Dahl-Jørge, O Skog, O Korsgren Characterisation of the endocrine pancreas in type 1 diabetes: islet size is maintained but islet number is markedly reduced J Pathol Clin Res, 2019-09-07;5(4):248-255. 2019-09-07 [PMID: 31493350] (IHC-P, Human) IHC-P Human
Lam CJ, Chatterjee A, Shen E et al. Low Level Insulin Content Within Abundant Non-Beta Islet Endocrine Cells in Long-Standing Type 1 Diabetes Diabetes 12/14/2018 [PMID: 30552110]
Show All 11 Publications.

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Array AF7068

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Bioinformatics

Gene Symbol ARX
Uniprot