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ARX Products By Gene Name

8 Results for "ARX"

AF7068

ARX Antibody

(11 Publications)

100 μg

AF7068

Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma cell line, U-118-MG human glioblastoma/astrocytoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ARX at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ARX was detected in immersion fixed U-87 MG human glioblastoma/astrocytoma cell line using Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

ARX was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF7068-SP

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NBP2-80506

ARX Antibody

0.1 ml

NBP2-80506

Western Blot: ARX Antibody [NBP2-80506] - WB on mouse brain lysate. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:1500 dilution incubated overnight at 4C.

Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506] - IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506] - IHC-P on paraffin sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506] - IHC-P on paraffin sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506] - IHC-P on paraffin sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506] - IHC-P on paraffin sections of mouse olfactory bulb. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506] - IHC-P on paraffin sections of mouse olfactory bulb. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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H00170302-M03

ARX Antibody (1G11)

0.1 mg

H00170302-M03

Immunocytochemistry/Immunofluorescence: ARX Antibody (1G11) [H00170302-M03] - Analysis of monoclonal antibody to ARX on HeLa cell. Antibody concentration 10 ug/ml

ELISA: ARX Antibody (1G11) [H00170302-M03] - Detection limit for recombinant GST tagged ARX is approximately 1ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00170302-M04

ARX Antibody (4H8)

0.1 mg

H00170302-M04

Immunohistochemistry-Paraffin: ARX Antibody (4H8) [H00170302-M04] - Analysis of monoclonal antibody to ARX on formalin-fixed paraffin-embedded human skeletal muscle. Antibody concentration 3 ug/ml

ELISA: ARX Antibody (4H8) [H00170302-M04] - Detection limit for recombinant GST tagged ARX is approximately 0.3ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-75093

ARX Antibody

100 μg

NBP2-75093

Western Blot: ARX Antibody [NBP2-75093] - (0.5ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Reactivity:

Hu, Mu, Rt, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Ca

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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H00170302-M08

ARX Antibody (4A8)

0.1 mg

H00170302-M08

Western Blot: ARX Antibody (4A8) [H00170302-M08] - Analysis of ARX expression in SJCRH30 (Cat # L027V1).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00170302-M02

ARX Antibody (4D12)

0.1 mg

H00170302-M02

Sandwich ELISA: ARX Antibody (4D12) [H00170302-M02] - Detection limit for recombinant GST tagged ARX is approximately 0.1ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00170302-M01

ARX Antibody (1G2)

0.1 mg

H00170302-M01

Sandwich ELISA: ARX Antibody (1G2) [H00170302-M01] - Detection limit for recombinant GST tagged ARX is approximately 0.3ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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