![Western Blot: CDKL5 Antibody [NBP2-32157] - Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDKL5 antibody NBP2-32157 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CDKL5-Antibody-Western-Blot-NBP2-32157-img0001.jpg "Western Blot: CDKL5 Antibody [NBP2-32157] - Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDKL5 antibody NBP2-32157 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen this antibody was made to, maps to a region between residue 350 to 400 of human Cyclin-Dependent Kinase-Like 5 using the numbering given in entry NP_003150.1 (GeneID 6792). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CDKL5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for CDKL5 Antibody (NBP2-32157)Find related products by research area.
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| Gene Symbol | CDKL5 |
| Uniprot |
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![Immunohistochemistry-Paraffin: CDKL5 Antibody [NBP2-32157] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- CDKL5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/CDKL5-Antibody-Immunohistochemistry-Paraffin-NBP2-32157-img0006.jpg "Immunohistochemistry-Paraffin: CDKL5 Antibody [NBP2-32157] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- CDKL5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).")
![Immunohistochemistry-Paraffin: CDKL5 Antibody [NBP2-32157] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- CDKL5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/CDKL5-Antibody-Immunohistochemistry-Paraffin-NBP2-32157-img0005.jpg "Immunohistochemistry-Paraffin: CDKL5 Antibody [NBP2-32157] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- CDKL5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).")
![Immunoprecipitation: CDKL5 Antibody [NBP2-32157] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDKL5 antibody NBP2-32157 used for IP at 6 ug/mg lysate. CDKL5 was inefficiently immunoprecipitated by rabbit anti-CDKL5 antibody BL15151. For blotting immunoprecipitated CDKL5, NBP2-32157 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CDKL5-Antibody-Immunoprecipitation-NBP2-32157-img0002.jpg "Immunoprecipitation: CDKL5 Antibody [NBP2-32157] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDKL5 antibody NBP2-32157 used for IP at 6 ug/mg lysate. CDKL5 was inefficiently immunoprecipitated by rabbit anti-CDKL5 antibody BL15151. For blotting immunoprecipitated CDKL5, NBP2-32157 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
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![Immunohistochemistry Netrin-G1a Antibody [Unconjugated]](https://images.novusbio.com/images/af1166_mouse-netrin-g1a-affinity-purified-polyclonal-ab-8220249503271.jpg)
![Immunocytochemistry/ Immunofluorescence Netrin-G1a Antibody [Unconjugated]](https://images.novusbio.com/images/af1166_mouse-netrin-g1a-affinity-purified-polyclonal-ab-8220249503240.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")