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Syntaxin-BP1 Antibody (6D1)

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Western Blot: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - STXBP1 monoclonal antibody (M01), clone 6D1. Analysis of STXBP1 expression in NIH/3T3.
Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - Analysis of monoclonal antibody to STXBP1 on formalin-fixed paraffin-embedded human cerebellum. Antibody concentration 3 ug/ml.
Western Blot: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - STXBP1 monoclonal antibody (M01), clone 6D1 Analysis of STXBP1 expression in HeLa.
Western Blot: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - STXBP1 monoclonal antibody (M01), clone 6D1. Analysis of STXBP1 expression in Raw 264.7.
Western Blot: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - Analysis of STXBP1 expression in transfected 293T cell line by STXBP1 monoclonal antibody (M01), clone 6D1.Lane 1: STXBP1 transfected lysate (Predicted MW: ...read more
Sandwich ELISA: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - Detection limit for recombinant GST tagged STXBP1 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
6D1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Syntaxin-BP1 Antibody (6D1) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
STXBP1 (NP_003156, 74 a.a. ~ 168 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VYLITPSEKSVHSLISDFKDPPTAKYRAAHVFFTDSCPDALFNELVKSRAAKVIKTLTEINIAFLPYESQVYSLDSADSFQSFYSPHKAQMKNPI
Specificity
STXBP1 (6D1)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
STXBP1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Publications
Read Publication using H00006812-M01.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Syntaxin-BP1 Antibody (6D1)

  • EIEE4
  • FLJ37475
  • hUNC18
  • MUNC18-1
  • NSEC1
  • N-Sec1
  • p67
  • Protein unc-18 homolog 1
  • Protein unc-18 homolog A
  • rbSec1
  • STXBP1
  • syntaxin binding protein 1
  • syntaxin-binding protein 1
  • SyntaxinBP1
  • Syntaxin-BP1
  • UNC18
  • unc18-1
  • UNC18A
  • unc-18A
  • UNC18neuronal SEC1

Background

Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part to its ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc-18 is thought to be regulated by PKC phosphorylation of Ser515 on the Munc-18 protein (Sassa et al., 1996).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Syntaxin-BP1 Antibody (H00006812-M01)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol STXBP1
Entrez
OMIM
Uniprot