Name: Neurogenin-3 Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF3444
Price: 489 USD

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, IHC
Clonality	Polyclonal
Host	Sheep
Conjugate	Unconjugated
Concentration	LYOPH

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF3444

Immunogen	E. coli-derived recombinant human Neurogenin-3 Met1-Leu214 Accession # Q9Y4Z2
Specificity	Detects human Neurogenin-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Neurogenin-3 is observed, and less than 1% cross-reactivity with recombinant human Neurogenin-1 is observed.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Sheep
Gene	NEUROG3
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Neurogenin-3 Antibody [Unconjugated]

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Publications for Neurogenin-3 Antibody (AF3444)(19)

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Bioinformatics

Publications using AF3444	Applications	Species
Sara Jiménez, Valérie Schreiber, Reuben Mercier, Gérard Gradwohl, Nacho Molina Characterization of cell-fate decision landscapes by estimating transcription factor dynamics Cell Reports Methods 2023-07-24 [PMID: 37533652]
Miguel-Escalada I, Maestro MA, Balboa D et al. Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster Developmental cell 8/22/2022 [PMID: 35998583] Details: Supplementary figure 5
Hyo Jeong Yong, Gengqiang Xie, Chengyang Liu, Wei Wang, Ali Naji, Jerome Irianto et al. Gene Signatures of NEUROGENIN3+ Endocrine Progenitor Cells in the Human Pancreas Front Endocrinol (Lausanne) 2021-09-08 [PMID: 34566896]
NJ Hogrebe, KG Maxwell, P Augsornwor, JR Millman Generation of insulin-producing pancreatic beta cells from multiple human stem cell lines Nature Protocols, 2021-08-04;0(0):. 2021-08-04 [PMID: 34349281] (Flow Cytometry, ICC, Human)	Flow Cytometry, ICC	Human
L Velazco-Cr, MM Goedegebuu, KG Maxwell, P Augsornwor, NJ Hogrebe, JR Millman SIX2 Regulates Human &beta Cell Differentiation from Stem Cells and Functional Maturation Inï¿½Vitro Cell Rep, 2020-05-26;31(8):107687. 5/26/2020 [PMID: 32460030]
NK Vinckier, NA Patel, RJ Geusz, A Wang, J Wang, I Matta, AR Harrington, M Wortham, N Wetton, J Wang, US Jhala, MG Rosenfeld, CW Benner, HP Shih, M Sander LSD1-mediated enhancer silencing attenuates retinoic acid signalling during pancreatic endocrine cell development Nat Commun, 2020-04-29;11(1):2082. 2020-04-29 [PMID: 32350257] (ICC, Human, Mouse)	ICC	Human, Mouse
NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells Nat. Biotechnol., 2020-02-24;0(0):. 2/24/2020 [PMID: 32094658]
JR Alvarez-Do, J Donaghey, N Rasouli, JHR Kenty, A Helman, J Charlton, JR Straubhaar, A Meissner, DA Melton Circadian Entrainment Triggers Maturation of Human Inï¿½Vitro Islets Cell Stem Cell, 2019-12-12;26(1):108-122.e10. 2019-12-12 [PMID: 31839570] (ICC, Human)	ICC	Human
N Sharon, J Vanderhoof, J Straubhaar, J Mueller, R Chawla, Q Zhou, EN Engquist, C Trapnell, DK Gifford, DA Melton Wnt Signaling Separates the Progenitor and Endocrine Compartments during Pancreas Development Cell Rep, 2019-05-21;27(8):2281-2291.e5. 2019-05-21 [PMID: 31116975] (Flow Cytometry, Human)	Flow Cytometry	Human
EA Rosado-Oli, K Anderson, JH Kenty, DA Melton YAP inhibition enhances the differentiation of functional stem cell-derived insulin-producing ? cells Nat Commun, 2019-04-01;10(1):1464. 2019-04-01 [PMID: 30931946] (Flow Cytometry, ICC, Human)	Flow Cytometry, ICC	Human
Diego Balboa, Jonna Saarimäki-Vire, Daniel Borshagovski, Mantas Survila, Päivi Lindholm, Emilia Galli et al. Insulin mutations impair beta-cell development in a patient-derived iPSC model of neonatal diabetes eLife 2018-11-09 [PMID: 30412052]
Cyrille Ramond, Belin Selcen Beydag-Tasöz, Ajuna Azad, Martijn van de Bunt, Maja Borup Kjær Petersen, Nicola L. Beer et al. Understanding human fetal pancreas development using subpopulation sorting, RNA sequencing and single-cell profiling Development 2018-03-13 [PMID: 30042179]
II Aigha, B Memon, AK Elsayed, EM Abdelalim Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors Stem Cell Res Ther, 2018-04-03;9(1):83. 2018-04-03 [PMID: 29615106] (ICC, Human)	ICC	Human
B Memon, M Karam, S Al-Khawaga, EM Abdelalim Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1 Stem Cell Res Ther, 2018-01-23;9(1):15. 1/23/2018 [PMID: 29361979]
NAJ Krentz, D van Hoof, Z Li, A Watanabe, M Tang, C Nian, MS German, FC Lynn Phosphorylation of NEUROG3 Links Endocrine Differentiation to the Cell Cycle in Pancreatic Progenitors Dev. Cell, 2017-04-24;41(2):129-142.e6. 2017-04-24 [PMID: 28441528] (Western Blot, Human)	Western Blot	Human
J Saarimäki-, D Balboa, MA Russell, J Saarikettu, M Kinnunen, S Keskitalo, A Malhi, C Valensisi, C Andrus, S Eurola, H Grym, J Ustinov, K Wartiovaar, RD Hawkins, O Silvennoin, M Varjosalo, NG Morgan, T Otonkoski An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation Cell Rep, 2017-04-11;19(2):281-294. 2017-04-11 [PMID: 28402852] (ICC, Human)	ICC	Human
Cai , Qing, Bonfanti , Paola, Sambathkumar , Rangaraj, Vanuytsel , Kim, Vanhove , Jolien, Gysemans , Conny, Debiec-Rychter , Maria, Raitano , Susanna, Heimberg , Harry, Ordovas , Laura, Verfaillie , Catherin Prospectively isolated NGN3-expressing progenitors from human embryonic stem cells give rise to pancreatic endocrine cells. Stem Cells Transl Med, 2014-02-03;3(4):489-99. 2014-02-03 [PMID: 24493854] (ICC, Human)	ICC	Human
Kayali AG, Lopez AD, Hao E, Hinton A, Hayek A, King CC The SDF-1ÃŽÂ±/CXCR4 Axis is Required for Proliferation and Maturation of Human Fetal Pancreatic Endocrine Progenitor Cells. PLoS ONE, 2012-06-22;7(6):e38721. 2012-06-22 [PMID: 22761699] (IHC-P, Human)	IHC-P	Human
Lina Sui, Josué K. Mfopou, Bing Chen, Karen Sermon, Luc Bouwens Transplantation of Human Embryonic Stem Cell-Derived Pancreatic Endoderm Reveals a Site-Specific Survival, Growth, and Differentiation Cell Transplantation 2013-05-01 [PMID: 22472700]
Show All 19 Publications. Collapse Publications.

Catalog# & Formulation	Size	Price
Carrier Free AF3444-SP

Gene Symbol	NEUROG3
Uniprot	Q9Y4Z2()