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RSK2 Antibody

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Western Blot: RSK2 Antibody [NBP2-20237] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membranes were blotted with RSK2 antibody and competitor's antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Independent Antibodies: Western Blot: RSK2 Antibody [NBP2-20237] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membranes were blotted with RSK2 antibody and competitor's antibody. ...read more
Immunohistochemistry-Paraffin: RSK2 Antibody [NBP2-20237] - Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.
Western Blot: RSK2 Antibody [NBP2-20237] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7. 5% SDS PAGE gel, diluted at 1:2000.
Western Blot: RSK2 Antibody [NBP2-20237] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: RSK2 Antibody [NBP2-20237] - Sample (30 ug of whole cell lysate) A: PC-12 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunohistochemistry-Paraffin: RSK2 Antibody [NBP2-20237] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: RSK2 Antibody [NBP2-20237] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

        

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RSK2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human RSK2. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPS6KA3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:20000
Theoretical MW
84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for RSK2 Antibody

  • 90 kDa ribosomal protein S6 kinase 3
  • CLS
  • Coffin-Lowry syndrome
  • EC 2.7.11
  • EC 2.7.11.1
  • HU-3
  • Insulin-stimulated protein kinase 1
  • ISPK1
  • ISPK-1
  • MAP kinase-activated protein kinase 1b
  • MAPK-activated protein kinase 1b
  • MAPKAP kinase 1b
  • MAPKAPK1B
  • MAPKAPK-1b
  • mental retardation, X-linked 19
  • MRX19
  • p90-RSK2
  • p90RSK3
  • pp90RSK2
  • ribosomal protein S6 kinase alpha-3
  • ribosomal protein S6 kinase, 90kD, polypeptide 3
  • ribosomal protein S6 kinase, 90kDa, polypeptide 3
  • Ribosomal S6 kinase 2
  • RPS6KA3
  • RSK
  • RSK2
  • RSK-2
  • RSK2p90-RSK 3
  • S6K-alpha3
  • S6K-alpha-3

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol RPS6KA3
Uniprot