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p70 S6 Kinase/S6K Antibody [Unconjugated]

 (6 Publications)
 
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Western blot shows lysates of MCF-7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse/Rat p70 S6 Kinase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8962) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for p70 S6 Kinase at approximately 70 and 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of MCF-7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Rabbit ...read more
HeLa human cervical epithelial carcinoma cell line was stained with Rabbit Anti-Human/Mouse/Rat p70 S6 Kinase Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF8962, filled histogram) or control antibody ...read more
p70 S6 Kinase was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat p70 S6 Kinase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8962) at ...read more
p70 S6 Kinase was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat p70 S6 Kinase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8962) at ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for p70 S6 Kinase at ...read more
Time-dependent changes in autophagic induction with mild MPP+ exposure.(a) SH-SY5Y cells were exposed to 10 and 200 μM MPP+ for up to 48 h. Time-dependent changes in the phosphorylation levels of various proteins ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, CyTOF-ready, Flow
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF8962-SP
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p70 S6 Kinase/S6K Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human p70 S6K
Accession # M60725
Specificity
Detects human, mouse, and rat p70 S6K and p85 S6K (also known as p70 S6K alpha I), an isoform with 23 extra residues at the N-terminus. Reactivity with  beta isoforms of p70 S6K is unknown.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPS6KB1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunohistochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF8962 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p70 S6 Kinase/S6K Antibody [Unconjugated]

  • EC 2.7.11
  • EC 2.7.11.1
  • p70 ribosomal S6 kinase alpha
  • p70 S6 kinase alpha
  • p70 S6 Kinase
  • p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1
  • p70 S6 kinase, alpha 2
  • p70 S6KA
  • p70 S6K-alpha
  • p70(S6K)-alpha
  • p70-alpha
  • p70-S6K
  • P70S6K1
  • PS6K
  • ribosomal protein S6 kinase beta-1
  • Ribosomal protein S6 kinase I
  • ribosomal protein S6 kinase, 70kD, polypeptide 1
  • ribosomal protein S6 kinase, 70kDa, polypeptide 1
  • RPS6KB1
  • S6K
  • S6K1
  • S6K1p70-S6K 1
  • S6K-beta-1
  • serine/threonine kinase 14 alpha
  • Serine/threonine-protein kinase 14A
  • STK14A

Background

p70 S6 kinase is a Ser/Thr kinase activated by such mitogens as EGF, IGF-I, and insulin. The S6 protein of the 40S ribosomal subunit is a major substrate of p70 S6 kinase, and its phosphorylation upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start site. The activity of p70 S6 kinase is controlled by multiple phosphorylation events, with phosphorylation at T229 by PDK1 and T389 by mTOR most critical for kinase function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p70 S6 Kinase/S6K Antibody (AF8962)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(3)
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(3)
Mouse
(1)
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Showing Publications 1 - 6 of 6.
Publications using AF8962 Applications Species
Qian F, Nettleford SK, Zhou J et al. Activation of GPR44 decreases severity of myeloid leukemia via specific targeting of leukemia initiating stem cells Cell reports 7/25/2023 [PMID: 37459233]
R Ji, L Ma, X Chen, R Sun, L Zhang, H Saiyin, W Wei Characterizing the distributions of IDO-1 expressing macrophages/microglia in human and murine brains and evaluating the immunological and physiological roles of IDO-1 in RAW264.7/BV-2 cells PLoS ONE, 2021-11-04;16(11):e0258204. 2021-11-04 [PMID: 34735466] (Western Blot, Human) Western Blot Human
V Sharma, M Makhdoomi, L Singh, P Kumar, N Khan, S Singh, HN Verma, K Luthra, S Sarkar, D Kumar Trehalose limits opportunistic mycobacterial survival during HIV co-infection by reversing HIV-mediated autophagy block Autophagy, 2020-02-20;0(0):1-20. 2020-02-20 [PMID: 32079455] (Human) Human
S Sakamoto, M Miyara, S Sanoh, S Ohta, Y Kotake Mild MPP(+) exposure-induced glucose starvation enhances autophagosome synthesis and impairs its degradation Sci Rep, 2017-04-26;7(0):46668. 2017-04-26 [PMID: 28443637] (Western Blot, Human) Western Blot Human
Fang Liu, Yuan Zhang, Tingting Men, Xingyue Jiang, Chunhua Yang, He Li et al. Quantitative proteomic analysis of gastric cancer tissue reveals novel proteins in platelet-derived growth factor B signaling pathway Oncotarget 3/28/2017 [PMID: 28423550]
Nakai A, Yamaguchi O, Takeda T The role of autophagy in cardiomyocytes in the basal state and in response to hemodynamic stress. Nat. Med., 2007-04-22;13(5):619-24. 2007-04-22 [PMID: 17450150] (Western Blot, Mouse) Western Blot Mouse

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S6K is a serine/threonine kinase that is a member of the ribosomal S6 kinase (RSK) family. S6K exists in two main isoforms, S6K1 and S6K2, which can also be alternatively spliced to produce different splice forms. S6K1 has two major splicing produ...  Read full blog post.
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Bioinformatics

Gene Symbol RPS6KB1
Uniprot